A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) in southern Africa

Timm, Alicia E.; Stiller, Michael; Frey, Jürg E.

doi:10.4001/1021-3589-16.1.68

Cited by 29 publications

(15 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Identification of most thrips to a species level is difficult due to their small size, subtle morphological differences 47 , 48 , high intraspecific polymorphism 49 , sexual dimorphism 50 , and the presence of cryptic species and species complexes 48 , 51 , 52 . Molecular identification of Thysanoptera species is desirable as it overcomes these complexities.…”

Section: Discussionmentioning

confidence: 99%

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Kaczmarczyk

Kucharczyk

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Insects’ exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial communities of four developmental stages of the fungivorous species Hoplothrips carpathicus (Thysanoptera: Phlaeothripidae), verification of the presence of Wolbachia, in silico prediction of metabolic potentials of the microorganisms, and sequencing its mitochondrial COI barcode. Taxonomy-based analysis indicated that the bacterial community of H. carpathicus contained 21 bacterial phyla. The most abundant phyla were Proteobacteria, Actinobacteria, Bacterioidetes and Firmicutes, and the most abundant classes were Alphaproteobacteria, Actinobacteria, Gammaproteobacteria and Betaproteobacteria, with different proportions in the total share. For pupa and imago (adult) the most abundant genus was Wolbachia, which comprised 69.95% and 56.11% of total bacterial population respectively. Moreover, similarity analysis of bacterial communities showed that changes in microbiome composition are congruent with the successive stages of H. carpathicus development. PICRUSt analysis predicted that each bacterial community should be rich in genes involved in membrane transport, amino acid metabolism, carbohydrate metabolism, replication and repair processes.

show abstract

Section: Discussionmentioning

confidence: 99%

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Kaczmarczyk

Kucharczyk

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Thus, it is necessary to build robust reference profiles and an agreed framework for the sharing and quality control of the sequence data in order to adopt DNA barcoding on a global scale [ 31 ], as well as to allow the classification of crop pest species collected from different vegetables and locations [ 44 ]. According to the available literature, the use of genetic markers, such as mtDNA, represents at present, a valuable addition or alternative to the classical methods of species identification [ 24 , 33 , 43 , 47 , 49 , 50 , 51 ], especially when the morphological approach is difficult or even impossible.…”

Section: Discussionmentioning

confidence: 99%

DNA Barcoding: A Reliable Method for the Identification of Thrips Species (Thysanoptera, Thripidae) Collected on Sticky Traps in Onion Fields

Marullo

Mercati

Vono

2020

Insects

View full text Add to dashboard Cite

Several thrips species (Insecta, Thysanoptera) are globally known as important crop pests and vectors of viral diseases, but their identification is difficult because of their small body size and inconspicuous morphological differences. Sequencing variation in the mitochondrial cytochrome c oxidase I (COI) region has been proven to be useful for the identification of species of many groups of insect pests. Here, DNA barcoding has been used to identify thrips species collected with the use of sticky traps placed in an open onion field. A total of 238 thrips specimens were analyzed, 151 of which could be identified to species and 27 to genera belonging to the family Thripidae. Fifty-one specimens could not be assigned to any genus, with the closest BLAST match in the GenBank queries being below 98%, whilst six specimens were not recognized as Thysanoptera. The results indicate that, although there are a few pest thrips species not yet barcoded, most of the species that may cause damage to crops in Europe are represented in GenBank and other databases, enabling correct identification. Additionally, DNA barcoding can be considered a valuable alternative to the classic morphology method for identification of major thrips species.

show abstract

“…Based on nucleotide sequence analysis of the mitochondrial COI gene, a system of identifying economically important thrips species present in Europe (Brunner et al 2002) and in southern Africa (Timm et al 2008) was developed. Brunner et al (2002) used sequencing instead of RFLP to analyse the PCR products.…”

Section: Methods Based On Dna Analysismentioning

confidence: 99%

Traditional and modern methods for the identification of thrips (Thysanoptera) species

Mehle

Trdan

2012

J Pest Sci

View full text Add to dashboard Cite

Many thrips are pests of commercial crops due to the damage they cause by feeding on developing flowers or vegetables. Thrips may also serve as vectors for plant diseases, such as tospoviruses. Their small size and predisposition towards enclosed places makes them difficult to detect by phytosanitary inspection. In this review, several methods available for identifying thrips, including their advantages and disadvantages, are discussed. A combination of different methods gives the most reliable identification. Relatively new morphometric, molecular and biochemical methods for identifying thrips species represent valuable alternatives for situations in which correct identification with classical morphological methods is very difficult, time consuming or virtually impossible. However, traditional morphological methods should not be neglected, especially because adequate identification using morphological keys is usually an indispensable first step in the development and validation of these new modern methods. In addition, modern systems may still require specimen identification to the genus level via morphological keys, or such keys may be recommended to confirm the results of modern identification methods.

show abstract

A molecular identification key for economically important thrips species (Thysanoptera: Thripidae) in southern Africa

Cited by 29 publications

References 19 publications

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

DNA Barcoding: A Reliable Method for the Identification of Thrips Species (Thysanoptera, Thripidae) Collected on Sticky Traps in Onion Fields

Traditional and modern methods for the identification of thrips (Thysanoptera) species

Contact Info

Product

Resources

About