A molecular phylogeny of Aeridinae (Orchidaceae: Epidendroideae) inferred from multiple nuclear and chloroplast regions

Zou, Long−Hai; Huang, Jiu-Xiang; Zhang, Guo‐Qiang; Liu, Zhong‐Jian; XueYing, Zhuang

doi:10.1016/j.ympev.2015.02.014

Cited by 40 publications

(61 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Within this subtribe, low support is observed, and the relationships are difficult to infer. Recent molecular phylogenetic analyses of Aeridinae (Zou et al, ) are based on five DNA sequences (ITS, atpI‐H , matK , psbA‐trnH , and trnL‐F ), and most Chinese taxa indicate that the subtribe is primarily grouped into 10 clades. However, the relationships between most of these clades remain unresolved.…”

Section: Discussionmentioning

confidence: 99%

A molecular phylogeny of Chinese orchids

Zhang

Lan

et al. 2016

J of Sytematics Evolution

Self Cite

View full text Add to dashboard Cite

We estimated the molecular phylogenetic relationships of the Chinese members of the family Orchidaceae. Within the Tree of Life for the Genera of Chinese Vascular Plants using atpB, rbcL, matK, ndhF, and matR, the currently delimited subfamilies, tribes, and subtribes are highly supported as monophyletic except for the perplexing Epidendroideae. Five genes (rbcL, matK, psaB, ycf1, and Xdh), which are more universally used in Orchidaceae, were further analyzed to reconstruct the phylogeny of Epidendroideae. The reconstructed trees were in strong agreement and showed significant support for the tribal and subtribal clades. Based on the highly supported circumscription and arrangement of the suprageneric levels in the Tree of Life and reconstructed trees, we have proposed a new phylogenetic classification of Chinese Orchidaceae that includes five subfamilies, 17 tribes, and 21 subtribes.

show abstract

Section: Discussionmentioning

confidence: 99%

A molecular phylogeny of Chinese orchids

Zhang

Lan

et al. 2016

J of Sytematics Evolution

Self Cite

View full text Add to dashboard Cite

show abstract

“…The number of accepted species varies around one hundred [ 1 , 2 ], with the most recent estimate of 88 [ 3 , 4 ]. Molecular analyses support the placement of the genus into the subtribe Aeridinae, tribe Vandeae, subfamily Epidendroideae of Orchidaceae [ 3 , 5 – 9 ]. Despite its well-supported phylogenetic position, the traditional morphologically delimited genus Cleisostoma seems to be polyphyletic [ 3 , 5 – 7 , 9 – 10 ].…”

Section: Introductionmentioning

confidence: 88%

“…Total genomic DNA was extracted using the DNeasy 96 Plant kit (Qiagen, Venlo, The Netherlands) following the manufacturer´s instructions. Polymerase chain reaction (PCR) amplifications were carried out as described in [ 7 ] and [ 9 ] using the set of oligonucleotide primers used by [ 9 ]. Similarity searches against the GenBank database were performed with the obtained sequence data using the Blast+ algorithm [ 36 ] in order to check for potential fungal contamination and find sequences of related species.…”

Section: Methodsmentioning

confidence: 99%

“…Aeridinae are one of the most complicated subtribes within Orchidaceae. The subtribe encompasses more than 1300 species in 83 genera [ 3 ], many of which are of notorious taxonomic difficulty [ 3 , 9 , 11 , 12 ]. Because robust phylogenetically-based taxonomic revision of the entire group is still lacking, we follow here conventional morphological circumscription of the genus, similarly to the most recent complete treatment of Orchidaceae [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A New Species of Cleisostoma (Orchidaceae) from the Hon Ba Nature Reserve in Vietnam: A Multidisciplinary Assessment

et al. 2016

View full text Add to dashboard Cite

A new species, Cleisostoma yersinii J. Ponert & Vuong, is described and illustrated based on the material collected in the Hon Ba Nature Reserve in southern Vietnam. In addition to conventional (macro)morphological examination we comparatively investigated root and leaf anatomy (using light and fluorescent microscopy), assessed nectar characteristics (using HPLC analysis), determined nuclear genome size (using DNA flow cytometry) and reconstructed phylogenetic relationships (using nrITS sequences). Cleisostoma yersinii differs from its putative closest relative C. birmanicum in wider and shorter leaves, larger flowers, distinct lip with S-shaped tip of the mid-lobe, and a shallow spur with two large nectar sacks separated by prominent calli and septum. Nectar is sucrose-dominant and very rich in sugars. Stomata are developed on both sides of the leaf and have prominent hyperstomatal chambers and substomatal cavities. Roots with well-developed exodermis and tracheoidal idioblasts are covered by a two-layer Vanda-type velamen. Chloroplasts occur not only in the cortex but are also abundant in the stele. Mean 1C-value was estimated to 2.57 pg DNA. An updated identification key is provided for SE Asian sections and all Vietnamese species of Cleisostoma.

show abstract

“…It is important to correctly estimate and understand intraspecific variations in cpDNA (Wang et al, 2004;Kozyrenko et al, 2009). cpDNA sequences have been used as important markers in systematic research and have shown superiority in medicinal plant research (Hosokawa et al, 2005;Zou et al, 2015). Therefore, 130 C. officinalis specimens from Henan, Shaanxi, and other main origins were collected in this experiment and subjected to cpDNA extraction.…”

Section: Introductionmentioning

confidence: 99%

A SCAR marker for the analysis of chloroplast DNA from different cultivars of Cornus officinalis

Zhang

Wang

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The aims of this study were to establish a random amplified polymorphic DNA (RAPD) fingerprint database of chloroplast DNA (cpDNA) from different cultivars of Cornus officinalis and to convert RAPD markers to sequence characterized amplified regions (SCAR) markers. A method of extraction was established that was suitable for obtaining cpDNA from samples rapidly dried in silicone; an RAPD fingerprint database was built; and the genetic distance between samples was used as statistical clustering variables for calculating DICE genetic similarity coefficients and for building a kinship tree chart. RAPD markers were converted to SCAR markers to design specific primers, and samples from C. officinalis cultivars, plants of the same family, and its adulterants, were used for amplification and identification. Fifteen amplified primers with stable polymorphisms were screened for amplification of 130 copies of materials. In total, 57 sites were achieved, 40 of which were polymorphic, and the polymorphic rate was up to 70.18%. A genetic tree was built based on (2015) seven cultivars. SCAR markers of C. officinalis cpDNA were successfully converted into RAPD markers. cpDNA samples from hawthorn, C. officinalis, Cornus wood, and grape were used for SCAR amplification, and their bands were distinctly different. In conclusion, SCAR markers and cpDNA may be used for research on C. officinalis and its adulterants, and the results may provide a basis for identifying germplasm and screening fine varieties at a molecular level.

show abstract

A molecular phylogeny of Aeridinae (Orchidaceae: Epidendroideae) inferred from multiple nuclear and chloroplast regions

Cited by 40 publications

References 16 publications

A molecular phylogeny of Chinese orchids

A molecular phylogeny of Chinese orchids

A New Species of Cleisostoma (Orchidaceae) from the Hon Ba Nature Reserve in Vietnam: A Multidisciplinary Assessment

A SCAR marker for the analysis of chloroplast DNA from different cultivars of Cornus officinalis

Contact Info

Product

Resources

About