A Molecular Probe for the Detection of Homopurine Sequences

Pyrene-modified PNAs: Stacking interactions and selective excimer emission in PNA2DNA triplexes

“…Furthermore, pyrene has been shown to favour self-assembly processes of supramolecular structures [21–28] and interact with carbon nanostructures such as nanotubes [29] or graphene [30], thus allowing to create composite material with special properties. PNA fluorescent probes bearing pyrene units as “universal base” were described [31–32], and recently, pyrrolidinyl-PNA bearing a uracil-linker pyrene unit showed good fluorescence response and mismatch recognition [33]; though terminal pyrene units were shown to stabilize triplexes formed by oligonucleotide probes [34], the effect of single- or multiple pyrene units on PNA in the formation of triplex structures has still to be addressed.…”

Triplex Molecular Beacons as Modular Probes for DNA Detection

“…In this setup, only one dual-labeled probe is needed and it is the sfO that allows tuning of the selectivity and sensitivity of the tMB towards target DNA sequences. Furthermore, the modular assembly [5] facilitates the additional incorporation of functionalities that can be easily introduced via the sfO. In an example of this concept, we prepared "superquenched" [6] tMBs by appending additional quencher moieties to the sfO.…”

Triplex‐basierte “molecular beacons” als modulare Sonden zur DNA‐Detektion

“…Dieser Aufbau benötigt nur eine doppelt markierte Sonde, da Selektivität und Empfindlichkeit der DNA‐Detektion über die Wahl des sfO justiert werden können. Darüber hinaus ermöglicht der modulare Aufbau5 den Einbau weiterer Funktionalitäten, die sehr einfach über das sfO eingeführt werden können. Ein Beispiel für dieses erweiterte Konzept sind “superquenched”6 tMBs, die durch Anbindung zusätzlicher Fluoreszenzlöscher an das sfO erhalten wurden.…”