A Molecular Study with A Comparison of the Odds of Diagnostic Methods For Burkholderia Cepacia Bacteria Isolated from Patients with Diabetic Foot Ulcer

Abstract

The study aim to isolation and identification of Gram negative aerobic bacteria from clinical samples from various pathological conditions, investigation the virulence factors genes of bacteria. The present study included 250 specimens collected from patients suffering from diabetic foot ulcer , cystic fibrosis , people exposed to burns to different degrees , People with eye infections, in addition to those with fistulas , who attends to Alsader medical city, Al-Hakim General Hospital during the period extended from September 2020 to February 2021 for both sexes with an age ranged between )1 -75) years. The results revealed that 20 specimens of the total number of samples are Burkholderia cepacia . The results revealed that the frequency among males 198 (79.2%) more than female 52 (20.8%). The sample distribution according age, it appears high 7.6% with group 1-15 years in female , and high 22% with group 31-45 years in male, while high 26.8% with group 31-45 years male and female .

Molecular detection of some virulence factors of hypervirulent Klebsiella pneumonia that associated with pathogenicity

“…Bacterial surfaces are engulfed using capsular layers, which inhibit phagocytosis.The most extensive capsular antigens are K1 and K2 [16]. The carbohydrate-rich pill of K. Pneumonia will increase pathogenicity and shields the micro organism inside from leukocyte absorption and the serum's bactericidal results [16] These findings corroborated those of Aljanaby and Alhasany (17,18,19). The outcomes of this study accept as true with AL-Salem and Bunyan's [19] finding that K. Pneumoniae can make siderophores in 92.86% of all isolates.…”

Detection of the Serum Level of C3 and C4 Among Patients with Urinary Tract Infection

“…UTI is greatly caused via Gram-negative bacterial agents like Klebsiella species, Escherichia coli, Pseudomonas aeruginosa, Proteus species and Acinetobacter species [8]. Among the Gram-positive bacteria, Enterococcus species and Staphylococcus saprophyticus are the most common predicted bacteria responsible for UTI [9].…”