A molecular-thermodynamic model for the interactions between globular proteins in aqueous solutions: Applications to bovine serum albumin (BSA), lysozyme, α-chymotrypsin, and immuno-gamma-globulins (IgG) solutions

Identification and Partial Characterization of Two Populations of Prostasomes by a Combination of Dynamic Light Scattering and Proteomic Analysis

“…Figure 1c shows the typical result obtained when the correlation functions are analyzed in terms of size distributions: two distinct subpopulations clearly appeared, both in the absence and in the presence of albumin. In the latter case (as in this example), the free protein was also clearly distinguishable, with a radius of the order of &6.5 nm, which is consistent with the literature data (Jin et al 2006;Vilker et al 1979).…”

Carboxylate-Dependent Gelation of a Monoclonal Antibody

“…Bundling is also possible through a counterion effect in which filament surface charges are neutralized, followed by filament-alignment by Van der Waal interactions (41), a phenomenon also described by the interaction potential and the Yukawa model of interacting proteins (42,43). The two-Yukawa potential has been used to predict the osmotic pressure of IgG proteins; however, this model is best used in solutions with low ionic strength and small self-association (44). On the other hand, in strongly associating systems, these parameters may not be predictive.…”

Principles, approaches, and challenges for predicting protein aggregation rates and shelf life

“…113,114 Therefore, one often must assume a physically reasonable form for Dg ðaÞ 12 ðrÞ and approximate or fit model parameters to best describe the experimental system of interest. 20,111,[115][116][117][118] Fundamentally, K ðUÞ 2 is directly related to k obs (Tab. 1 and Fig.…”

“…(14)-(16), the folded-state quantities therefore serve as rigorously based, albeit surrogate, parameters to inform indirectly on k obs .Figure 2and the calculations described below and in Supporting Information show qualitative and quantitative relationship between osmotic virial coefficients and equilibrium constants for native and non-native dimer formation. For simplicity and illustration purposes, Dg ðaÞ 12 ðrÞ is based on a colloidal interaction model typical of those used to model liquid-phase protein-protein interactions and phase behavior 20,112,[115][116][117][118]. The mathematical details of Dg ðaÞ 12 ðrÞ as used here are available elsewhere[116][117][118] and in Supporting Information.Briefly, DgðaÞ 12 ðrÞ incorporates…”

“…For simplicity and illustration purposes, Dg ðaÞ 12 ðrÞ is based on a colloidal interaction model typical of those used to model liquid-phase protein-protein interactions and phase behavior 20,112,[115][116][117][118]. The mathematical details of Dg ðaÞ 12 ðrÞ as used here are available elsewhere[116][117][118] and in Supporting Information.Briefly, DgðaÞ 12 ðrÞ incorporates…”