A Molecular Toolkit for Highly Insulating Tethered Bilayer Lipid Membranes on Various Substrates

Atanasov, Vladimir; Atanasova, Petia; Vockenroth, Inga K.; Knorr, Nikolaus; Köper, Ingo

doi:10.1021/bc050328n

Cited by 65 publications

(82 citation statements)

References 42 publications

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“…Lipid monolayers were formed via self-assembly by immera͒ sion of an ultra flat gold substrate into a diluted solution of the anchorlipid DPhyTL ͑0.2 mg/mL͒, which was synthesized as described previously. 21,27,28 DPhyTL consists of two phytanyl chains that are coupled via a glycerol linker to a tetraethylene oxide spacer. The molecules are grafted to the gold surface by sulfur-gold bonds via a lipoic acid moiety.…”

Section: Methodsmentioning

confidence: 99%

“…C SC represents the space charge capacitance due to the spacer region combined with the capacitive effects of the electrochemical double layer at the gold interface. 21,24 All electrochemical experiments were performed in 0.1 M KCl unless otherwise noted. For the long time measurements, the solution has been replaced before each measurement, ensuring that the membrane did not dry out.…”

Section: B Electrochemical Impedance Spectroscopy "Eis…mentioning

confidence: 99%

“…18 Tethered bilayer lipid membranes ͑tBLMs͒ are versatile model systems that provide a defined platform to study membrane related processes and membrane proteins. [19][20][21][22][23] They provide excellent stability by covalently linking the bilayer membrane to a solid support. First demonstrated by Cornell et al, the inner leaflet of the membrane is bound to the solid support via a short spacer group.…”

Section: Introductionmentioning

confidence: 99%

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Stable insulating tethered bilayer lipid membranes

et al. 2008

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Tethered bilayer lipid membranes have been shown to be an excellent model system for biological membranes. Coupling of a membrane to a solid supports creates a stable system that is accessible for various surface analytical tools. Good electrical sealing properties also enable the use of the membranes in practical sensing applications. The authors have shown that tethered membranes have extended lifetimes up to several months. Air-stability of the bilayer can be achieved by coating the membrane with a hydrogel. The structure of a monolayer and its stability under applied dc potentials have been investigated by neutron scattering. © 2008 American Vacuum Society.

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Section: Methodsmentioning

confidence: 99%

Section: B Electrochemical Impedance Spectroscopy "Eis…mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Stable insulating tethered bilayer lipid membranes

et al. 2008

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“…C SC represents the space charge capacitance due to the spacer region combined with the capacitive effects of the electrochemical double layer at the gold interface. 28,35 In the case of low monolayer resistances, an additional resistance attributed to the spacer region can be seen; therefore, an R͑RC͒͑RC͒ circuit has been used. 36 EIS data can be visualized in Bode plots ͑see Fig.…”

Section: Electrochemical Impedance Spectroscopymentioning

confidence: 99%

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

et al. 2009

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Tethered bilayer lipid membranes are promising biomimetic architectures. Their formation has been investigated using four different surface sensitive techniques, including optical, acoustic, and electrical methods. The lipid bilayers are built in a two-step procedure; the proximal layer is formed by self-assembly and is then completed to a bilayer by fusion with small vesicles. The different technical approaches revealed specific aspects of the layer formation processes, namely, first a fast adsorption process followed by a longer rearrangement period. Similar phenomena have been observed for the vesicle fusion process. The results allow for a more controlled assembly protocol for the preparation of highly insulating lipid membranes.

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“…[289] Using thiole-terminated anchor lipids with an oligoethylene glycol spacer, such membranes can be assembled onto gold surfaces. [270,290] The proximity of the substrate provides mechanical stability necessary for long term applications, e.g. in sensing devices.…”

Section: Introductionmentioning

confidence: 99%

Surface Patterning with Colloidal Monolayers

Vogel

2012

Springer Theses

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This thesis focuses on the controlled assembly of monodisperse polymer colloids into ordered two-dimensional arrangements. These assemblies, commonly referred to as colloidal monolayers, are subsequently used as masks for the generation of arrays of complex metal nanostructures on solid substrates.The motivation of the research presented here is twofold. First, monolayer crystallization methods were developed to simplify the assembly of colloids and to produce more complex arrangements of colloids in a precise way. Second, various approaches to colloidal lithography are designed with the aim to include novel features or functions to arrays of metal nanostructures.The air/water interface was exploited for the crystallization of colloidal monolayer architectures as it combines a two-dimensional confinement with a high lateral mobility of the colloids that is beneficial for the creation of high long range order. A direct assembly of colloids is presented that provides a cheap, fast and conceptually simple methodology for the preparation of ordered colloidal monolayers. The produced two-dimensional crystals can be transformed into nonclose-packed architectures by a plasma-induced size reduction step, thus providing valuable masks for more sophisticated lithographic processes. Finally, the controlled co-assembly of binary colloidal crystals with defined stoichiometries on a Langmuir trough is introduced and characterized with respect to accessible configurations and size ratios.Several approaches to lithography are presented that aim at introducing different features to colloidal lithography. First, using metal-complex containing latex particles, the synthesis of which is described as well, symmetric arrays of metal nanoparticles can be created by controlled combustion of the organic material of the colloids. The process does not feature an inherent limit in nanoparticle size and is able to produce complex materials as will be demonstrated for FePt alloy particles. Precise control over both size and spacing of the particle array is presented.Second, two lithographic processes are introduced to create sophisticated nanoparticle dimer units consisting of two crescent shaped nanostructures in close proximity; essentially by using a single colloid as mask to generate two structures simultaneously. Strong coupling processes of the parental plasmon resonances of the two objects are observed that are accompanied by high near-field enhancements. A plasmon hybridization model is elaborated to explain all polarization dependent shifts of the resonance positions. Last, a technique to produce laterally patterned, ultra-flat substrates without surface topographies by embedding gold nanoparticles in a silicon dioxide matrix is applied to construct robust and re-usable sensing architectures and to introduce an approach for the nanoscale patterning of solid supported lipid bilayer membranes.

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A Molecular Toolkit for Highly Insulating Tethered Bilayer Lipid Membranes on Various Substrates

Cited by 65 publications

References 42 publications

Stable insulating tethered bilayer lipid membranes

Stable insulating tethered bilayer lipid membranes

Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques

Surface Patterning with Colloidal Monolayers

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