A Monoclonal Antibody Produced Against Naked2

Cao, Chang; Wang, Shengyu; Lv, Sha; Li, Zhe; Wang, Xianjiang; Zeng, Fanwei; Zhang, Haipeng; Dai, Yujuan; Dou, Xiaofeng; Chen, Xiaoli; Li, Xiudong; Luo, lingli; Hu, Tianhui; Yan, Jianghua

doi:10.1089/mab.2012.0111

Cited by 2 publications

(2 citation statements)

References 12 publications

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“…Anti‐human NKD2 antibody (R44) and TGFα polyclonal antibody were generated by Covance . Mouse NKD2 antibody was a kind gift from Tianhui Hu and Jianhua Yan's Lab . AKAP12 antibody (cat#: PA5‐21759, RRID:AB_11154150), Protein G beads and Mammalian Protein Extraction Reagent (M‐PER) were purchased from ThermoFisher Scientific and mouse AKAP12 antibody from Abcam (ab49849, RRID:AB_2225608).…”

Section: Methodsmentioning

confidence: 99%

Protein kinase A‐mediated phosphorylation of naked cuticle homolog 2 stimulates cell‐surface delivery of transforming growth factor‐α for epidermal growth factor receptor transactivation

Cao

Singh

Li³

et al. 2019

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The classic mode of G protein‐coupled receptor (GPCR)‐mediated transactivation of the receptor tyrosine kinase epidermal growth factor receptor (EGFR) transactivation occurs via matrix metalloprotease (MMP)‐mediated cleavage of plasma membrane‐anchored EGFR ligands. Herein, we show that the Gαs‐activating GPCR ligands vasoactive intestinal peptide (VIP) and prostaglandin E2 (PGE2) transactivate EGFR through increased cell‐surface delivery of the EGFR ligand transforming growth factor‐α (TGFα) in polarizing madin‐darby canine kidney (MDCK) and Caco‐2 cells. This is achieved by PKA‐mediated phosphorylation of naked cuticle homolog 2 (NKD2), previously shown to bind TGFα and direct delivery of TGFα‐containing vesicles to the basolateral surface of polarized epithelial cells. VIP and PGE2 rapidly activate protein kinase A (PKA) that then phosphorylates NKD2 at Ser‐223, a process that is facilitated by the molecular scaffold A‐kinase anchoring protein 12 (AKAP12). This phosphorylation stabilized NKD2, ensuring efficient cell‐surface delivery of TGFα and increased EGFR activation. Thus, GPCR‐triggered, PKA/AKAP12/NKD2‐regulated targeting of TGFα to the cell surface represents a new mode of EGFR transactivation that occurs proximal to ligand cleavage by MMPs.

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Section: Methodsmentioning

confidence: 99%

Protein kinase A‐mediated phosphorylation of naked cuticle homolog 2 stimulates cell‐surface delivery of transforming growth factor‐α for epidermal growth factor receptor transactivation

Cao

Singh

Li³

et al. 2019

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“…Two of three DE genes in almost all studied comparisons were clustered here. The Naked 2 gene (NKD2) encodes a protein, which is a member of Naked family protein and a negative regulator of canonical Wnt signaling(CAO et al, 2013), a proliferative pathway. The NKD2 expression gradually increased during diestrus, being highest on day 60 p.o.…”

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<b>Mecanismo de ação do 17β-estradiol no corpo lúteo de cadelas não prenhes</b>

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A Monoclonal Antibody Produced Against Naked2

Cited by 2 publications

References 12 publications

Protein kinase A‐mediated phosphorylation of naked cuticle homolog 2 stimulates cell‐surface delivery of transforming growth factor‐α for epidermal growth factor receptor transactivation

Protein kinase A‐mediated phosphorylation of naked cuticle homolog 2 stimulates cell‐surface delivery of transforming growth factor‐α for epidermal growth factor receptor transactivation

<b>Mecanismo de ação do 17β-estradiol no corpo lúteo de cadelas não prenhes</b>

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A Monoclonal Antibody Produced Against Naked2

Cited by 2 publications

References 12 publications

Protein kinase A‐mediated phosphorylation of naked cuticle homolog 2 stimulates cell‐surface delivery of transforming growth factor‐α for epidermal growth factor receptor transactivation

Protein kinase A‐mediated phosphorylation of naked cuticle homolog 2 stimulates cell‐surface delivery of transforming growth factor‐α for epidermal growth factor receptor transactivation

<b>Mecanismo de ação do 17&#946;-estradiol no corpo lúteo de cadelas não prenhes</b>

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<b>Mecanismo de ação do 17β-estradiol no corpo lúteo de cadelas não prenhes</b>