A monoclonal antibody specific for a unique biomarker, virenose, in a lipopolysaccharide of Coxiella burnetii

Palkovičová, Katarína; Ihnatko, Robert; Vadovič, Pavol; Betinová, Eva; Škultéty, Ľudovít; Frangoulidis, Dimitrios; Toman, Rudolf

doi:10.1111/j.1469-0691.2008.02218.x

Cited by 12 publications

(9 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several PI-LPS specific mAbs have been developed for analysis of antigenicity of immunogenic components of C. burnetii LPS and detection of the virulent form of C. burnetii (33–36). Hotta et al (34) reported generation of 19 PI-LPS specific mAbs and demonstrated that these mAbs were able to be separated into three groups based on their reactivity with O-polysaccharide chains (27 kDa of PI-LPS), O-polysaccharide chains (15 to 27 kDa of PI-LPS) and outer-core oligosaccharides (14 kDa of PI-LPS).…”

Section: Discussionmentioning

confidence: 99%

“…Hotta et al (34) reported generation of 19 PI-LPS specific mAbs and demonstrated that these mAbs were able to be separated into three groups based on their reactivity with O-polysaccharide chains (27 kDa of PI-LPS), O-polysaccharide chains (15 to 27 kDa of PI-LPS) and outer-core oligosaccharides (14 kDa of PI-LPS). Palkovicova et al (36) developed a virenose targeted mAb (IgG2b subclass) that recognized O-polysaccharide chains and suggested that this virenose unique mAb may be a useful biomarker for detection of virulent C. burnetii . However, it still remains unknown whether PI-LPS recognized mAbs are protective.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development of a Lipopolysaccharide-Targeted Peptide Mimic Vaccine against Q Fever

Peng

Zhang

Mitchell

et al. 2012

The Journal of Immunology

View full text Add to dashboard Cite

C burnetii is a gram-negative bacterium that causes acute and chronic Q fever in humans. Creation of a safe and effective new generation vaccine to prevent Q fever remains an important public health goal. Previous studies suggested that antibody (Ab)-mediated immunity to C. burnetii phase I lipopolysaccharide (PI-LPS) is protective. To identify the potential peptides that can mimic the protective epitopes on PI-LPS, a PI-LPS specific monoclonal antibody (mAb) 1E4 was generated, characterized and used to screen a phage display library. Interestingly, our results indicate that 1E4 was able to inhibit C. burnetii infection in vivo, suggesting that 1E4 is a protective mAb. After three rounds of biopanning by 1E4 from the phage display library, a mimetic peptide, m1E41920 was identified, chemically synthesized and conjugated to KLH for examining its immunogenicity. The results indicate that the synthetic peptide m1E41920 was able to inhibit the binding of 1E4 to PI antigen, suggesting m1E41920 shares the same binding site of 1E4 with the epitopes of PI antigen. In addition, m1E41920-KLH elicited a specific IgG response to PI antigen and immune sera from m1E41920-KLH-immunized mice was able to inhibit C. burnetii infection in vivo, suggesting that m1E41920 may specifically mimic the protective epitope of PI-LPS. Furthermore, m1E41920-KLH was able to confer significant protection against C. burnetii challenge. Thus, m1E41920-KLH is a protective antigen and may be useful for developing a safe and effective vaccine against Q fever. This study demonstrates the feasibility of developing a peptide mimic vaccine against Q fever.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of a Lipopolysaccharide-Targeted Peptide Mimic Vaccine against Q Fever

Peng

Zhang

Mitchell

et al. 2012

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…After decades of development, ELISA/EIA has become widely used for laboratory research as well as for clinical diagnosis. 102 It has also been reported that oligomannose-conjugated AuNPs detect antibody in serum by ELISA, demonstrating the diagnostic potential for detecting HIV infection. 99 Carbohydrate-based ELISAs have been exploited for many commercialized diagnostic assays.…”

Section: Enzyme-linked Assaysmentioning

confidence: 95%

Glycan-based diagnostic devices: current progress, challenges and perspectives

Wang

Cheng

2015

Chem. Commun.

View full text Add to dashboard Cite

Diagnosis is an essential launching point for accurate medical treatment, and rapid and accurate diagnostic approaches have become the increasingly vital goal inspiring developments in a variety of analytical methods. With increasing attention in the area of glycosciences, the roles that carbohydrates play in many diseases have been elucidated, and the use of carbohydrates as disease biomarkers has become an emerging diagnostics strategy. This review article does not intend to comprehensively include all carbohydrate-related diagnostics studies. We do, however, wish to describe carbohydrate recognition and the design of sensitive detecting devices, especially those developed recently, so that readers might better grasp the principles behind such devices and relevant detection strategies, particularly those favoring inexpensive, rapid, and point-of-care (POC) approaches.

show abstract

“…D'après l'analyse des liens glycosidiques, il est prédit que les Vir, Strep et D-mannose (Man) soient localisés en position terminale de la chaine O-spécifique du LPS I (Narasaki et Toman 2012). Ces 2 sucres n'ont pas été retrouvés dans le LPS d'autres bactéries et sont considérés comme des biomarqueurs uniques de C. burnetii (Palkovicova et al 2009). …”

Section: Variation De Phaseunclassified

Coxiella burnetii, agent de la fièvre Q

Boarbi¹,

Frétin²,

Mori³

2016

Can. J. Microbiol.

View full text Add to dashboard Cite

Résumé : La fièvre Q est une zoonose de répartition mondiale excepté la Nouvelle-Zélande. Elle est due à une bactérie intracellulaire : Coxiella burnetii. Cette infection passe souvent inaperçue car la manifestation principale de la forme aiguë de la maladie est un syndrome pseudogrippal spontanément résolutif. C'est l'épidémie qui a eu lieu aux Pays-Bas qui a attiré l'attention sur cette maladie qui était moins étudiée auparavant. Cette synthèse résume la description de la bactérie (taxonomie, cycle intracellulaire et génomique) mais également la maladie (description, diagnostic, pathogénie et épidémiologie). Enfin, la vaccination de l'humain et des animaux est également analysée. Mots-clés : Coxiella burnetii, fièvre Q, épidémiologie, description, génomique.Abstract: Q fever is a zoonosis of worldwide distribution with the exception of New Zealand. It is caused by an intracellular bacterium, Coxiella burnetii. The disease often goes underdiagnosed because the main manifestation of its acute form is a general self-limiting flu-like syndrome. The Dutch epidemics renewed attention to this disease, which was less considered before. This review summarizes the description of C. burnetii (taxonomy, intracellular cycle, and genome) and Q fever disease (description, diagnosis, epidemiology, and pathogenesis). Finally, vaccination in humans and animals is also considered.

show abstract

A monoclonal antibody specific for a unique biomarker, virenose, in a lipopolysaccharide of Coxiella burnetii

Cited by 12 publications

References 3 publications

Development of a Lipopolysaccharide-Targeted Peptide Mimic Vaccine against Q Fever

Development of a Lipopolysaccharide-Targeted Peptide Mimic Vaccine against Q Fever

Glycan-based diagnostic devices: current progress, challenges and perspectives

Coxiella burnetii, agent de la fièvre Q

Contact Info

Product

Resources

About