A Morphologic and Fluorophotometric Analysis of the Corneal Endothelium in Type I Diabetes Mellitus and Cystic Fibrosis

Lass, Jonathan H.; Spurney, Robert V.; Dutt, R. M.; Andersson, Henry; Kochar, Hermahinder; Rodman, Harvey M.; Stern, Robert C.; Doershuk, Carl F.

doi:10.1016/s0002-9394(14)73367-7

Cited by 84 publications

(33 citation statements)

References 22 publications

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“…[13][14][15][16] However, some investigators have reported no significant differences between diabetic and normal corneal endothelial cells after cataract surgery. [4][5][6] The change in corneal endothelial cell morphology after cataract surgery in diabetes, especially in patients with diabetic retinopathy, is still controversial. [17][18][19][20] Thus, we investigated the change of corneal endothelial morphology after phacoemulsification with IOL implantation in diabetes according to the severity of diabetic retinopathy and we compared these changes with those in age-matched nondiabetic patients.…”

mentioning

confidence: 99%

Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Lee

Choi

et al. 2005

Journal of Cataract and Refractive Surgery

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mentioning

confidence: 99%

Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Lee

Choi

et al. 2005

Journal of Cataract and Refractive Surgery

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“…4 In controlled in vivo experiments, these methods have been used to evaluate the outcome of various treatments: corneal transplantation, 5 refractive 6 -9 or cataract surgery, 10,11 drugs, 12,13 contact lenses, 14 -29 or intraocular lenses. 30 It has also been used to monitor the progression of a disease with ocular effects, 31 the influence of age, 32-36 trauma, 37 or the severity of a developmental disease, which appears correlated with endothelial appearance. 38 For these studies to be able to discriminate between two treatments, the morphologic method used must have the smallest experimental error possible, be accurate, and be precise.…”

mentioning

confidence: 99%

Morphometry of Cells and Guttae in Subjects With Normal or Guttate Endothelium With a Contour Detection Algorithm

Giasson

Graham²,

Blouin³

et al. 2005

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

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“…Clinical studies have also shown that Cystic Fibrosis patients have normal corneal thickness and corneal endothelial morphology, consistent with little or no role in baseline corneal endothelial function. Interestingly, the endothelial cell density of CF patients may be slightly higher than in controls (Lass et al 1985). Possibly this is to compensate for the defective channel.…”

Section: Discussionmentioning

confidence: 92%

Dependence of cAMP meditated increases in Cl− and HCO3− permeability on CFTR in bovine corneal endothelial cells

Allen

Sun

et al. 2008

Experimental Eye Research

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The Cystic Fibrosis Transmembrane conductance Regulator (CFTR) is present on the apical membrane of corneal endothelial cells. Increasing intracellular [cAMP] with forskolin stimulates an NPPB and glibenclamide-inhibitable apical Cl -and HCO 3 -permeability. To definitively determine that the increased permeability is dependent on CFTR, we used an siRNA knockdown approach. Apical Cl -and HCO 3 -permeability and steady-state HCO 3 -flux were measured in the presence or absence of forskolin using cultured bovine corneal endothelial cells that were transfected with CFTR siRNA or a scrambled sequence control. CFTR protein expression was reduced by ∼80% in CFTR siRNA treated cultures. Forskolin (10 μM) increased apical chloride permeability by 7 fold, which was reduced to control level in siRNA treated cells. CFTR siRNA treatment had no effect on baseline apical chloride permeability. Apical HCO 3 -permeability was increased 2 fold by 10 μM forskolin, which was reduced to control level in siRNA treated cultures. Similarly, there was no effect on baseline apical HCO 3 -permeability by knocking down CFTR expression. The steady-state apicalbasolateral pH gradient (ΔpH) at four hours in control cultures was increased ∼2.5 fold by forskolin. In CFTR siRNA treated cells, the baseline ΔpH was similar to control, however forskolin did not have a significant effect. We conclude that forskolin induced increases in apical HCO 3 -permeability in bovine corneal endothelium requires CFTR. However, CFTR does not have a major role in determining baseline apical chloride or HCO 3 -permeability.The corneal endothelium is responsible for maintaining the hydration and transparency of the cornea by counteracting the fluid imbibition properties of the glycosaminoglycan rich stroma. Numerous studies have shown that this process is dependent on the presence of HCO 3 -and slowed by inhibition of carbonic anhydrase activity (Hodson 1971;Barfort and Maurice 1974;Fischbarg and Lim 1974;Hodson 1974;Kuang et al. 1990;Riley et al. 1995). The endothelium develops a small apical side negative transendothelial potential (0.5 mV) that is significantly reduced by HCO 3 -withdrawal or by carbonic anhydrase inhibitors (Fischbarg 1972;Barfort and Maurice 1974;Fischbarg and Lim 1974;Hodson et al. 1977). Corneal swelling is also induced by the Na + /K + ATPase inhibitor ouabain, indicating that the endothelial pump involves active transport.HCO 3 -uptake at the basolateral (stromal) membrane has been identified as being predominantly via the 1Na + :2HCO 3 -cotransporter (NBCe1) (Sun et al. 2000;Sun and Bonanno 2003). A recent study showed that siRNA knockdown of NBC significantly reduced basolateral HCO 3 -permeability and eliminated net basolateral to apical fluxes (Li et al. 2005). Interestingly, studies using cultured cells indicate that basolateral HCO 3 -permeability is 3-4 times greater than apical permeability (Bonanno et al. 1999). The low HCO 3 -permeability of the apical membrane suggests that it is rate limiting for HCO 3 -fluxes and tha...

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A Morphologic and Fluorophotometric Analysis of the Corneal Endothelium in Type I Diabetes Mellitus and Cystic Fibrosis

Cited by 84 publications

References 22 publications

Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Corneal endothelial cell change after phacoemulsification relative to the severity of diabetic retinopathy

Morphometry of Cells and Guttae in Subjects With Normal or Guttate Endothelium With a Contour Detection Algorithm

Dependence of cAMP meditated increases in Cl− and HCO3− permeability on CFTR in bovine corneal endothelial cells

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