2017
DOI: 10.1016/j.exer.2017.04.003
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A mouse ocular explant model that enables the study of living optic nerve head events after acute and chronic intraocular pressure elevation: Focusing on retinal ganglion cell axons and mitochondria

Abstract: We developed an explant model of the mouse eye and optic nerve that facilitates the study of retinal ganglion cell axons and mitochondria in the living optic nerve head (ONH) in an ex vivo environment. Two transgenic mouse strains were used, one expressing yellow fluorescent protein in selected axons and a second strain expressing cyan fluorescent protein in all mitochondria. We viewed an explanted mouse eye and optic nerve by laser scanning microscopy at and behind the ONH, the site of glaucoma injury. Explan… Show more

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Cited by 21 publications
(20 citation statements)
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“… 7 , 39 41 Similarly, in larger mammalian eyes and humans, transport block in axons occurs in the connective tissue zone of the LC and myelination begins just posterior to this. 3 , 42 – 44 The accumulation of amyloid precursor protein 33 during axonal obstruction can lead to axonal swelling, 39 , 45 resulting in the increase in axonal area measured in this work.…”
Section: Discussionmentioning
confidence: 85%
“… 7 , 39 41 Similarly, in larger mammalian eyes and humans, transport block in axons occurs in the connective tissue zone of the LC and myelination begins just posterior to this. 3 , 42 – 44 The accumulation of amyloid precursor protein 33 during axonal obstruction can lead to axonal swelling, 39 , 45 resulting in the increase in axonal area measured in this work.…”
Section: Discussionmentioning
confidence: 85%
“…In an initial, pilot study describing this method (Kimball et. al., 2017), explants from young RGC-YFP mice were used to measure axonal integrity after acute and chronic pressure elevation.…”
Section: 0 Resultsmentioning
confidence: 99%
“…Twenty-nine explants were from 4 month old mice of the Mito-CFP strain, 18 explants were from 14 month old Mito-CFP mice, and 31 explants were from 14–17 month old RGC-YFP mice. For comparison to the older RGC-YFP mice, we utilized comparable data on younger (4 months old) mice, published previously (Kimball et al, 2017).…”
Section: 0 Methodsmentioning
confidence: 99%
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