A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

Koch, Suzanne E; Haaften, Eline E. van; Wissing, Tamar B.; Cuypers, Lizzy A B; Bulsink, Jurgen A.; Bouten, Cvc Carlijn; Kurniawan, Nicholas A.; Smits, Anthal I.P.M.

doi:10.3791/61824

Cited by 9 publications

(6 citation statements)

References 19 publications

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“…The contractile balloon models underwent inflation–deflation cycles (of air) at a 60 beat per minute (BPM) rate (recapitulating that of the adult human heart [ 33 ] ) using a programmed bioreactor (Ibidi, USA). [ 34 ] For this purpose, the ATES was adhered to the collagen sheet (no glue), and then the assembly was glued to the surface of the silicon balloon. The whole system was immersed into deionized (DI) water.…”

Section: Methodsmentioning

confidence: 99%

Extrusion‐Based 3D Bioprinting of Adhesive Tissue Engineering Scaffolds Using Hybrid Functionalized Hydrogel Bioinks

et al. 2023

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Adhesive tissue engineering scaffolds (ATESs) have emerged as an innovative alternative means, replacing sutures and bioglues, to secure the implants onto target tissues. Relying on their intrinsic tissue adhesion characteristics, ATES systems enable minimally invasive delivery of various scaffolds. This study investigates development of the first class of 3D bioprinted ATES constructs using functionalized hydrogel bioinks. Two ATES delivery strategies, in situ printing onto the adherend versus printing and then transferring to the target surface, are tested using two bioprinting methods, embedded versus air printing. Dopamine-modified methacrylated hyaluronic acid (HAMA-Dopa) and gelatin methacrylate (GelMA) are used as the main bioink components, enabling fabrication of scaffolds with enhanced adhesion and crosslinking properties. Results demonstrate that dopamine modification improved adhesive properties of the HAMA-Dopa/GelMA constructs under various loading conditions, while maintaining their structural fidelity, stability, mechanical properties, and biocompatibility. While directly printing onto the adherend yields superior adhesive strength, embedded printing followed by transfer to the target tissue demonstrates greater potential for translational applications. Together, these results demonstrate the potential of bioprinted ATESs as off-the-shelf medical devices for diverse biomedical applications.

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Section: Methodsmentioning

confidence: 99%

Extrusion‐Based 3D Bioprinting of Adhesive Tissue Engineering Scaffolds Using Hybrid Functionalized Hydrogel Bioinks

et al. 2023

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“…Prior to cell seeding, culture medium was removed and 3.8*10 6 ODMCs/graft were seeded using fibrin as a cell carrier as described before. [ 31 ] In short, cells were suspended in a mixture of fibrinogen (bovine, 10 mg mL −1 , Sigma) and thrombin (bovine, 10 IU mL −1 , Sigma) and homogeneously dripped over both the lumen and adventitial side of the graft. To promote a uniform distribution of cells, the cell‐loaded constructs were rotated by 180° every 15 min in a general incubator (37 °C, 5% CO 2 ) for 1 h. After 1 h polymerization at 37 °C, SMGM2 medium with 0.25 mg mL −1 ascorbic acid was added to induce ECM production and ODMCs were further cultured in static conditions for 48 h.…”

Section: Methodsmentioning

confidence: 99%

3D Human iPSC Blood Vessel Organoids as a Source of Flow‐Adaptive Vascular Cells for Creating a Human‐Relevant 3D‐Scaffold Based Macrovessel Model

et al. 2022

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Abstract3D‐scaffold based in vitro human tissue models accelerate disease studies and screening of pharmaceutics while improving the clinical translation of findings. Here is reported the use of human induced pluripotent stem cell (hiPSC)‐derived vascular organoid cells as a new cell source for the creation of an electrospun polycaprolactone‐bisurea (PCL‐BU) 3D‐scaffold‐based, perfused human macrovessel model. A separation protocol is developed to obtain monocultures of organoid‐derived endothelial cells (ODECs) and mural cells (ODMCs) from hiPSC vascular organoids. Shear stress responses of ODECs versus HUVECs and barrier function (by trans endothelial electrical resistance) are measured. PCL‐BU scaffolds are seeded with ODECs and ODMCs, and tissue organization and flow adaptation are evaluated in a perfused bioreactor system. ODECs and ODMCs harvested from vascular organoids can be cryopreserved and expanded without loss of cell purity and proliferative capacity. ODECs are shear stress responsive and establish a functional barrier that self‐restores after the thrombin challenge. Static bioreactor culture of ODECs/ODMCs seeded scaffolds results in a biomimetic vascular bi‐layer hierarchy, which is preserved under laminar flow similar to scaffolds seeded with primary vascular cells. HiPSC‐derived vascular organoids can be used as a source of functional, flow‐adaptive vascular cells for the creation of 3D‐scaffold based human macrovascular models.

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“…For the 3D experiments, cells were seeded in electrospun PCL-BU scaffold, using fibrin as a cell carrier, as previously developed, 47 and as extensively described elsewhere. 48 Cells (monocultures: 30 • 10 6 macrophages/cm 3 or 15 • 10 6…”

Section: Cell Seeding In Scaffoldmentioning

confidence: 99%

Donor Heterogeneity in the Human Macrophage Response to a Biomaterial Under Hyperglycemia In Vitro

Koch

Verhaegh

Smink

et al. 2022

Tissue Engineering Part C: Methods

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A Multi-Cue Bioreactor to Evaluate the Inflammatory and Regenerative Capacity of Biomaterials under Flow and Stretch

Cited by 9 publications

References 19 publications

Extrusion‐Based 3D Bioprinting of Adhesive Tissue Engineering Scaffolds Using Hybrid Functionalized Hydrogel Bioinks

Extrusion‐Based 3D Bioprinting of Adhesive Tissue Engineering Scaffolds Using Hybrid Functionalized Hydrogel Bioinks

3D Human iPSC Blood Vessel Organoids as a Source of Flow‐Adaptive Vascular Cells for Creating a Human‐Relevant 3D‐Scaffold Based Macrovessel Model

Donor Heterogeneity in the Human Macrophage Response to a Biomaterial Under Hyperglycemia In Vitro

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