A Multi‐Signaling Performance for Simultaneous Surveillance and Accretion of Cysteine and Serine in Human Cancer Cell

Abstract

A unique fluorescence chemosensing method has been introduced to explain a new strategy for simultaneous selective determination of cysteine and serine in two different emission channels through an "on-off-on" mechanism. Dansyl chloride-picolylamine conjugate (DNPC) ensembles with Cu 2 + (DNPC-Cu 2 + ) and shows a turn-on fluorescence while binding with serine or cysteine. Fluorescence, NMR titrations and DFT calculations has been performed in order to understand the sensing mechanism and the electronic properties of the receptor-donor complex. The chemosensing method has been successfully applied in human cancer cells to detect and quantify cysteine and serine differentially.

Differential detection of aspartic acid in MCF-7 breast cancer cells

“…Due to their simplicity, high selectivity, sensitivity, low cost, easy implementation, and real-time detection, it is always preferable to use chemosensors as an expedient tool in biological and clinical examination. [22][23][24][25][26][27][28][29] Herein, we report a pyridine-carbazole framework (PCF) to detect aspartic acid in live cell systems. The PCF probe has been characterized using UV-visible, fluorescence, 1 H NMR, 13 C NMR, and high-resolution mass spectrometry spectra, for C 20 H 18 N 3 with calc.…”

“…Due to their simplicity, high selectivity, sensitivity, low cost, easy implementation, and real-time detection, it is always preferable to use chemosensors as an expedient tool in biological and clinical examination. 22–29…”