A multidisciplinary approach to diagnose naturally occurring bovine tuberculosis in Brazil

Marassi, Carla Dray; Medeiros, Luciana dos Santos; Figueiredo, Eduardo Eustáquio de Souza; Fonseca, Leila S.; Duarte, Rafael da Silva; Paschoalin, Vânia Margaret Flosi; Oelemann, Walter; Lilenbaum, Walter

doi:10.1590/s0100-736x2013000100004

Cited by 9 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…30 Milk can be contributed in identifying the diseased cattle, although about 50 percentage of technique's sensitivity would decrease. 31 Also, it would be missed; apparently, their benefit through an examination of a few diseased cattle that persist at large groups. 20 The results of Tables 2 and 3 revealed that in Wasit province, the morbidity rate was more than this reported in Dhi-Qar province.…”

Section: Discussionmentioning

confidence: 99%

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

Al-Fattli

2016

J. Contemp. Med. Sci.

View full text Add to dashboard Cite

Objectives This study was conducted to disclose the specific antibodies against M. bovis of bovine tuberculosis (bTB) in blood and milk serum samples, with detection of the most prevalent clinical signs in positive cows. Methods In some rural areas of two Iraqi provinces (Wasit and Dhi-Qar), 119 lactating cows were submitted to the clinical examination with obtaining of blood and milk to tested by using the IDEXX ELISA test. Results The overall seroprevalence in blood and milk was (20.16%) and (15.12%), respectively. In Wasit, the prevalence was (22.85%) and (15.71%), while in Dhi-Qar, the prevalence was (16.32%) and (14.28%) in blood and milk, respectively. As well as, marked significant differences in seroprevalence were observed between and within the two study's provinces and samples. According to clinical examination, a significant rising (P > 0.05) was revealed in respiratory disorders, decreasing in milk production, emaciation, rough hair coat and repetitive reproductive problems, whilst a significant decreasing (P < 0.05) in persistent feces abnormalities, mastitis, lymph nodes enlargement and loss of appetite. Conclusion The study demonstrated, for the first time in Iraq, the efficient of IDEXX ELISA, as a screening test in the detection of bTB in lactating cows by using blood and milk serum samples, and the competence of milk, as sample, in exhibition of infection. Also, the study exposed the high infection rate of bTB in cows of rural areas of Wasit and Dhi-Qar provinces.

show abstract

Section: Discussionmentioning

confidence: 99%

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

Al-Fattli

2016

J. Contemp. Med. Sci.

View full text Add to dashboard Cite

show abstract

“…Em trabalho já publicado por nosso grupo (Marassi et al 2013) com a sua aplicabilidade dentro da estratégia ante-mortem: (1) TCS = triagem, (2) TCS + TCC = triagem + teste confirmatório (conforme recomendação do MAPA), (3) TCC = diagnóstico isolado, (4) TCC+IFN = diagnóstico + teste confirmatório, (5) IFN = diagnósti-co isolado, (6) ELISA = diagnóstico isolado, (7) IFN+ELISA = diagnóstico + ferramenta complementar, (8) TCC+ELISA = diagnóstico + ferramenta complementar; ou post-mortem: (9) TCS+TCC = triagem + teste confirmatório (conforme recomendação do MAPA) + Cultura = diagnóstico confirmatório post-mortem, (10) TCS + TCC = triagem + teste confirmatório (conforme recomendação do MAPA) + Histopatologia = diagnóstico confirmatório post-mortem (Quadros 1 e 2).…”

Section: Desenho Do Estudounclassified

“…Apesar dos diversos estudos sobre a eficácia, vantagens e desvantagens dos diferentes protocolos de diagnóstico da tuberculose bovina (Waters et al 2011, Marassi et al 2013, são escassos os dados referentes aos custos do seu diagnóstico, e até o presente momento não existem estudos sobre custo-efetividade para esta enfermidade no Brasil. O objetivo deste estudo foi realizar uma avaliação econômica do uso de testes de diagnóstico utilizados ante-mortem e post-mortem em animais provenientes de um rebanho naturalmente infectado com tuberculose bovina.…”

Section: Introductionunclassified

Análise de custo-efetividade de protocolos no diagnóstico da tuberculose bovina em um rebanho naturalmente infectado

et al. 2016

Self Cite

View full text Add to dashboard Cite

Resumo: Em diversos países, a tuberculose bovina, causada por Mycobacterium bovis, é tanto um problema econômico quanto de saúde pública. Mundialmente, são implementados programas de erradicação da enfermidade com políticas baseadas em testes tuberculínicos e abate dos animais reativos, porém pouco se sabe sobre os custos e a efetividade das políticas de erradicação. O presente estudo avaliou a relação de custo-efetividade dos protocolos de diagnóstico da tuberculose bovina, em uma abordagem multidisciplinar, empregados em um rebanho naturalmente infectado. Após realização da análise de custo-efetividade (C/Ef) dos protocolos diagnósticos ante-mortem observou-se que o Teste Cervical Comparativo (C/Ef=4,68), quando utilizado isoladamente, é a escolha diagnóstica mais custo-efetiva para um rebanho naturalmente infectado. Para os protocolos confirmatórios de diagnóstico post-mortem a histopatologia (C/Ef=17,47) associada ao Teste Cervical Simples e Teste Cervical Comparativo foi a escolha mais custo-efetiva para os animais do rebanho estudado. Entretanto, o único protocolo eficaz em diagnosticar 100% dos animais infectados foi o uso em conjunto do teste humoral ELISA associado ao teste celular IFN.

show abstract

“…The test measures cell-mediated immune response (CMI). Additionally, as serological tests could detect anergic animal, they can be employed as complementary tools (Waters et al 2011, Marassi et al 2013). Many proteins have been suggested as capture antigens for specific immunoglobulins, and MPB70 and MPB83 showed encouraging results, achieving 63% of sensitivity and 98% of specificity (Lyashschenko et al 2001, Waters et al 2011.…”

Section: Introductionmentioning

confidence: 99%

“…In those conditions, the impact of PPD inoculation on humoral response, detected by ELISA, remains unclear. Furthermore, animals can be in different stages of the infection, which could interfere on the efficacy of SICCT (Alvarez et al 2012, Marassi et al 2013. In that way, the purpose of this study was to detect specific antibodies at MPB70/83 ELISAs in cows after injection of PPD.…”

Section: Introductionmentioning

confidence: 99%

Detection of specific antibodies in cows after injection of PPD

et al. 2014

Self Cite

View full text Add to dashboard Cite

A multidisciplinary approach to diagnose naturally occurring bovine tuberculosis in Brazil

Cited by 9 publications

References 33 publications

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

Análise de custo-efetividade de protocolos no diagnóstico da tuberculose bovina em um rebanho naturalmente infectado

Detection of specific antibodies in cows after injection of PPD

Contact Info

Product

Resources

About