A Multifunctional Hydrogel for Simultaneous Visible H₂O₂ Monitoring And Accelerating Diabetic Wound Healing

Abstract

Diabetic wound is one of the chronic wounds that is difficult to heal, and effective treatment of it still confronts a great challenge. Monitoring the variation of diabetic wound microenvironment (such as hydrogen peroxide (H2O2)) can understand the wound state and guide the wound management. Herein, we developed a multifunctional hydrogel with the abilities of monitoring the H2O2 concentration, alleviating oxidative stress and promoting wound healing, which was prepared by encapsulating manganese‐containing bioactive glass (MnBG) and CePO4:Tb in biocompatible Gelatin methacryloyl (GelMA) hydrogel (CPT‐MnBG‐Gel). On the one hand, the H2O2‐dependent fluorescence quenching effect of the CePO4:Tb contributed to visible monitoring of the H2O2 concentration of wounds via smartphone imaging, and the CPT‐MnBG‐Gel hydrogel could effectively monitor the H2O2 level of 10.35‐200 μmol L−1. On the other hand, MnBG could alleviate oxidative stress and promote the proliferation, migration and differentiation of fibroblasts and endothelial cells in vitro owing to the bioactive Mn and Si ions, and in vivo evaluation also demonstrated that the CPT‐MnBG‐Gel hydrogels could effectively accelerate wound healing. Hence, such multifunctional hydrogel is promising for diabetic wound management and accelerating wound healing.This article is protected by copyright. All rights reserved