A multifunctional non-viral vector for the delivery of MTH1-targeted CRISPR/Cas9 system for non-small cell lung cancer therapy

Wang, Yu; Tang, Yuzhe; Zhao, Xiaomei; Huang, Gui; Gong, Jinhong; Yang, Shu-di; Li, Hui; Wan, Wenjun; Jia, Chang-Hao; Chen, Gang; Xue-nong, Zhang

doi:10.1016/j.actbio.2022.09.046

Cited by 23 publications

(22 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The efficacy of NSCLC therapy was enhanced by efficient delivery of siRNA to tumor cells through redox‐mediated intracellular disassembly 167 . Furthermore, Wang et al 248 . prepared the multifunctional nonviral vector, PS@HA‐Lip, which could actively target tumor cells and deliver CRISPR/Cas9 plasmids to the nuclei of the cells.…”

Section: Applications Of Biomaterial‐based Gene Delivery Systemsmentioning

confidence: 99%

“…The efficacy of NSCLC therapy was enhanced by efficient delivery of siRNA to tumor cells through redox-mediated intracellular disassembly. 167 Furthermore, Wang et al 248 prepared the multifunctional nonviral vector, PS@HA-Lip, which could actively target tumor cells and deliver CRISPR/Cas9 plasmids to the nuclei of the cells. In addition, it inhibited the growth and induced apoptosis of NSCLC cells by disrupting MTH1 gene expression and reducing liver metastasis associated with NSCLC.…”

Section: Neoplasmsmentioning

confidence: 99%

“…This multifunctional vector contained a NLS of protamine sulfate and was modified with distearoyl-phosphoethanolamine-PEG-HA to endow the vector with the ability to actively target tumor cells. 248 In addition, the highly aggressive and frequent recurrence of lung adenocarcinoma (LAC) is one of the main causes of failed treatments and poor prognoses. Chiou et al 249 used PU short-branched PEI (PU-PEI) as a vector to deliver miR145 to LAC cancer stem cells (LAC-CSCs).…”

Section: Neoplasmsmentioning

confidence: 99%

“…In addition, it inhibited the growth and induced apoptosis of NSCLC cells by disrupting MTH1 gene expression and reducing liver metastasis associated with NSCLC. This multifunctional vector contained a NLS of protamine sulfate and was modified with distearoyl‐phosphoethanolamine‐PEG‐HA to endow the vector with the ability to actively target tumor cells 248 . In addition, the highly aggressive and frequent recurrence of lung adenocarcinoma (LAC) is one of the main causes of failed treatments and poor prognoses.…”

Section: Applications Of Biomaterial‐based Gene Delivery Systemsmentioning

confidence: 99%

See 3 more Smart Citations

Biomaterial‐based gene therapy

Gao

et al. 2023

MedComm

View full text Add to dashboard Cite

Gene therapy, a medical approach that involves the correction or replacement of defective and abnormal genes, plays an essential role in the treatment of complex and refractory diseases, such as hereditary diseases, cancer, and rheumatic immune diseases. Nucleic acids alone do not easily enter the target cells due to their easy degradation in vivo and the structure of the target cell membranes. The introduction of genes into biological cells is often dependent on gene delivery vectors, such as adenoviral vectors, which are commonly used in gene therapy. However, traditional viral vectors have strong immunogenicity while also presenting a potential infection risk. Recently, biomaterials have attracted attention for use as efficient gene delivery vehicles, because they can avoid the drawbacks associated with viral vectors. Biomaterials can improve the biological stability of nucleic acids and the efficiency of intracellular gene delivery. This review is focused on biomaterial‐based delivery systems in gene therapy and disease treatment. Herein, we review the recent developments and modalities of gene therapy. Additionally, we discuss nucleic acid delivery strategies, with a focus on biomaterial‐based gene delivery systems. Furthermore, the current applications of biomaterial‐based gene therapy are summarized.

show abstract

Section: Applications Of Biomaterial‐based Gene Delivery Systemsmentioning

confidence: 99%

Section: Neoplasmsmentioning

confidence: 99%

Section: Neoplasmsmentioning

confidence: 99%

Section: Applications Of Biomaterial‐based Gene Delivery Systemsmentioning

confidence: 99%

See 2 more Smart Citations

Biomaterial‐based gene therapy

Gao

et al. 2023

MedComm

View full text Add to dashboard Cite

show abstract

“…Most approaches are utilized via the electrostatic adsorption of plasmids. 6,24,25 However, coloading carriers with hydrophobic drugs of controllable size and stability remain difficult. Cationic copolymer nanomicelle consisting of hydrophilic poly(ethylenimine) (PEI) with positive charge and hydrophobic lipoic acid (LA) with reductive cleavage of disulfide bonds was developed to load hydrophobic drugs at the core.…”

mentioning

confidence: 99%

Steerable Microneedles Enabling Deep Delivery of Photosensitizers and CRISPR/Cas9 Systems for Effective Combination Cancer Therapy

Wang,

Chen,

Zhang

et al. 2023

Nano Lett.

Self Cite

View full text Add to dashboard Cite

Although gene therapy has shown prospects in treating triple-negative breast cancer, it is insufficient to treat such a malignant tumor. Herein, nanoparticles (NPs)-embedded dissolving microneedles (IR780-PL/pFBXO44@MNs) with steerable and flectional property were developed to achieve the codelivery of FBXO44-targeted CRISPR/Cas9 plasmids (pFBXO44) and hydrophobic photosensitizers. For improved NP penetration in tumor tissue, collagenase@MNs were preapplied to degrade the tumor matrix. Under light irradiation, IR780 exhibited remarkable phototherapy, while the escape efficiency of NPs from lysosomes was improved. pFBXO44 was subsequently released in tumor cell cytoplasm via reducing the disulfide bonds of NPs, which could specifically knock out the FBXO44 gene to inhibit the migration and invasion of tumor cells. As a result, tumor cells were eradicated, and lung metastasis was effectively suppressed. This micelle-incorporated microneedle platform broadens the potential of combining gene editing and photo synergistic cancer therapy.

show abstract

Functional Materials for Subcellular Targeting Strategies in Cancer Therapy: Progress and Prospects

Cheng,

Qu,

Jiang

et al. 2023

Advanced Materials

View full text Add to dashboard Cite

Neoadjuvant and adjuvant therapies have made significant progress in cancer treatment. However, tumor adjuvant therapy still faces challenges due to the intrinsic heterogeneity of cancer, genomic instability, and the formation of an immunosuppressive tumor microenvironment. Functional materials possess unique biological properties such as long circulation times, tumor‐specific targeting, and immunomodulation. The combination of functional materials with natural substances and nanotechnology has led to the development of smart biomaterials with multiple functions, high biocompatibilities, and negligible immunogenicities, which can be used for precise cancer treatment. Recently, subcellular structure‐targeting functional materials have received particular attention in various biomedical applications including the diagnosis, sensing, and imaging of tumors and drug delivery. Subcellular organelle‐targeting materials can precisely accumulate therapeutic agents in organelles, considerably reduce the threshold dosages of therapeutic agents, and minimize drug‐related side effects. This review provides a systematic and comprehensive overview of the research progress in subcellular organelle‐targeted cancer therapy based on functional nanomaterials. Moreover, it explains the challenges and prospects of subcellular organelle‐targeting functional materials in precision oncology. We believe that our review will serve as an excellent cutting‐edge guide for researchers in the field of subcellular organelle‐targeted cancer therapy.This article is protected by copyright. All rights reserved

show abstract

A multifunctional non-viral vector for the delivery of MTH1-targeted CRISPR/Cas9 system for non-small cell lung cancer therapy

Cited by 23 publications

References 46 publications

Biomaterial‐based gene therapy

Biomaterial‐based gene therapy

Steerable Microneedles Enabling Deep Delivery of Photosensitizers and CRISPR/Cas9 Systems for Effective Combination Cancer Therapy

Functional Materials for Subcellular Targeting Strategies in Cancer Therapy: Progress and Prospects

Contact Info

Product

Resources

About