A multilayer strategy is needed to uncover the clubroot pathogen mysteries

A Basic Guide to the Propagation and Manipulation of the Clubroot Pathogen, Plasmodiophora brassicae

“…One of the most significant limitations associated with clubroot disease management is the emergence of novel P. brassicae pathotypes with distinct virulence profiles. These pathotypes pose a challenge to the utilization of genetic resistance as the preferred strategy for managing clubroot, highlighting the need for a deeper understanding of genetic diversity in the field and the mechanisms employed by these new isolates to overcome resistance (Wu & Pérez-López, 2023). To achieve this, generating single spore isolates (SSIs) is crucial.…”

“…These isolates can be subsequently propagated and phenotyped. For instance, they can be used to rapidly test new brassica varieties for breeding purposes or employed in genome-wide association studies to identify new clubroot-resistance genes or the interactive partners within the clubroot pathogen that triggers resistance (Javed et al, 2023;Wu & Pérez-López, 2023). Several methods have been put forward and are in use to generate SSIs (Jones et al, 1982;Tinggal & Webster, 1981;Voorrips, 1996;Xue et al, 2008).…”

“…Clubroot disease primarily affects cruciferous plants, leading to the formation of galls or clubs in the roots of susceptible hosts (Javed et al., 2023). This disease poses a significant global threat to agricultural production and has serious implications for global food security (Wu & Pérez‐López, 2023). To tackle this issue, numerous countries worldwide are actively devising strategies to manage and control clubroot disease.…”