A multiplex-PCR assay for the authentication of mackerels of the genus Scomber in processed fish products

Catanese, Gaetano; Manchado, Manuel; Fernández-Trujillo, A.; Infante, Carlos

doi:10.1016/j.foodchem.2010.02.036

Cited by 33 publications

(21 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method is widely accepted for identification of fish species in both fresh samples and cooked products. Catanese et al (2010) had utilized a multiplex PCR assay to successfully authenticate mackerels in fish fillets and canned fish products. Armani et al (2012) had applied a conventional and a real-time multiplex PCR to identify fish species in fresh samples and a variety of processed products including marinades, pasta sauces and batter-fried fish cakes.…”

Section: Identification Of Puffer Fish Of the Genus Lagocephalus 217mentioning

confidence: 99%

“…This study aims to identify an effective primer set for a simple and reliable detection based on multiplex PCR method of the three most concerned puffer fish species of the Indo-West Pacific region namely L. lunaris, L. spadiceus and L. inermis. Two mitochondrial DNA (mtDNA) genes, the 12S ribosomal RNA (12S rRNA) and the Cyt b, which have been shown to be effective for species identification based on PCR product size differences in several Downloaded by [University of Windsor] at 18:07 19 November 2014 fish taxa, have been chosen for the setting up of the multiplex PCR method (Armani et al, 2012;Catanese et al, 2010;Lin and Hwang, 2008;Zhang et al, 2007).…”

Section: Identification Of Puffer Fish Of the Genus Lagocephalus 217mentioning

confidence: 99%

“…However, the multiplex PCR method has gained wide acceptance for identification of fish species in both fresh samples and cooked products due to its simplicity, speed, economy and sensitivity that far surpasses both DNAfree and DNA-based methods previously mentioned (Catanese et al, 2010;Gil, 2007). This technique has also been applied to authenticate several other species, including sea cucumbers (Zuo et al, 2012) and mackerel (Catanese et al, 2010), and to uncover meat scandals, such as venison (Zha et al, 2010) and abalone products (Chan et al, 2012).…”

Section: Pcr Amplificationmentioning

confidence: 99%

“…This technique has also been applied to authenticate several other species, including sea cucumbers (Zuo et al, 2012) and mackerel (Catanese et al, 2010), and to uncover meat scandals, such as venison (Zha et al, 2010) and abalone products (Chan et al, 2012). Therefore, multiplex PCR with a novel primer set (KUGEN-ILSspec) could be an effective tool for prevention Downloaded by [University of Windsor] at 18:07 19 November 2014…”

Section: Pcr Amplificationmentioning

confidence: 99%

See 3 more Smart Citations

Identification of Puffer Fish of the GenusLagocephalus: L. lunaris, L. spadiceusandL. inermis, Using Multiplex PCR

2014

View full text Add to dashboard Cite

A multiplex PCR assay was developed to identify widespread poison-containing puffer fishes of the genus Lagocephalus: L. lunaris, L. spadiceus and L. inermis, using a novel KUGEN_ILSspec primer set which is specific for 12S ribosomal RNA (12S rRNA) and Cytochrome b (Cyt b) genes. KUGEN_ILSspec set is composed of fish positive-amplified primers that produced a 289-bp fragment while L. lunaris-, L. spadiceus-and L. inermis-specific primers produced 123-, 196-and 493-bp fragments, respectively. The sensitivity of this primer set was found to be high as it was capable of amplifying targeted DNA at concentrations as low as 1 ng/µL. Moreover, the primer set was shown to amplify intact DNA and species-specific fragments from heat-processed food. Consequently, multiplex PCR technique in combination with KUGEN_ILSspec primer set could offer an effective measure for detecting poisonous puffer fish contamination in both fresh and processed fish products, which will greatly aid in public health control and law enforcing endeavors.

show abstract

Section: Identification Of Puffer Fish Of the Genus Lagocephalus 217mentioning

confidence: 99%

Section: Identification Of Puffer Fish Of the Genus Lagocephalus 217mentioning

confidence: 99%

Section: Pcr Amplificationmentioning

confidence: 99%

Section: Pcr Amplificationmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Puffer Fish of the GenusLagocephalus: L. lunaris, L. spadiceusandL. inermis, Using Multiplex PCR

2014

View full text Add to dashboard Cite

show abstract

“…Several studies have reported the use of species-specific PCR to identify a species in seafood. Catanese, Manchado, Fernández-Trujillo, and Infante (2010) developed a multiplex-PCR assay of the mitochondrial control region for authentication of mackerel of the genus Scomber in processed fish products. A PCR assay targeting the 12S ribosomal RNA gene or 5SrDNA gene was developed to confirm the source of fish fillets labeled as grouper, and grouper served at restaurants (Asensio, 2008;Asensio et al, 2009).…”

Section: Introductionmentioning

confidence: 99%