A multiscale model approach for cell growth for lipids and pigments production by Haematococcus pluvialis under different environmental conditions.

Structured population balances to support microalgae-based processes: Review of the state-of-art and perspectives analysis

“…Cell volume can be measured directly or indirectly by using different techniques. For microalgae, these include the application of a cell counter, which measures the volume directly, or image-based cell-meters, which can be used to measure the area, diameter, volume or other morphological parameters, and flow-cytometers, which can be used to measure indirectly cell size/volume [54] , [57] , [58] , [59] . Flow-cytometry is a high-throughput method which can easily allow collecting up to 10,000 events/s, while the reliability of the results should be carefully assessed since the calibration of the instrument is more difficult compared to coulter counter and image-analysis.…”

“…Particularly, as living cells were concerned, the cell volume (or, equivalently, the diameter or the surface area) was typically used as internal coordinate in PBE models [34] , [40] , [46] , [48] , [49] , [50] , [51] , [52] , [53] , [54] . This choice was mainly motivated by the wider availability of cell size/volume distribution data derived by the application of cell counter, image based cell-meters, and cytofluorimetry [54] , [57] , [58] , [59] . The use of the mass as an internal coordinate for PBE modelling of living cells is, in contrast, less common, even though some relevant papers demonstrated that it permits obtaining simpler expressions for the growth and source/sink terms [38] , [53] , [57] , [62] , [63] .…”

“…Motivated by this analysis, with only a few exceptions [59] , [64] , [65] , PBEs so far proposed in the microalgae literature were written using the cell mass as the internal coordinate [19] , [43] , [58] , [59] , [64] , [66] , [67] .…”