A mutagenicity and cytotoxicity study on Limonium effusum aqueous extracts by Allium, Ames and MTT tests

Eren, Yasin; Özata, Ahmet; Konuk, Muhsin; Akyıl, Dilek; Liman, Recep

doi:10.3103/s0095452715020024

Cited by 5 publications

(5 citation statements)

References 34 publications

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“…To measure cell viability, MTT assay has been widely used as reliable and convenient quantitative method though it has been reported as a less dependable assay compared to other tests like Crystal Violet Staining (CVS) (Śliwka, et al, 2016) and ATP assays in certain cases in few studies (Yang, et al, 2015). To detect the probable cytotoxic and mutagenic effects of some plant extracts, simultaneous MTT and Ames tests on bacterial, plant, and mammalian cells have been conducted in some researches (Eren, Özata, Konuk, Akyil, & Liman, 2015). Anyhow, some problems of industrial factories can be fixed with the help of MTT assay.…”

Section: Discussionmentioning

confidence: 99%

Determining Optimal Cell Density and Culture Medium Volume simultaneously in MTT Cell Proliferation Assay for Adherent Cancer Cell Lines

Moradi¹

2018

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Introduction:The MTT assay without optimization of the factors affecting cell metabolism can lead to false reports of the tested drugs. In this study, cell density and cell culture medium volume were optimized in a 96-well plate for Hepa1-6 cells as an adherent cell line. Methods: Hepa1-6 cells were seeded in the two different volumes, five cell densities, and three incubation times. After the incubation times, the optical densities were read at 570nm. The absorbances of wells in 100 and 150µl of cell culture medium volumes were compared and the optimum cell density was funded through the mean absorbance analysis of wells in the optimum volume. Results: The Independent sample t-test showed a significant difference (p-value=0.01) between the absorbances of wells with 100 and 150 µl of volumes. Also, Pearson's correlation coefficient indicated that the optimum cell culture medium was 150 µl. The optimum cell density was 5000 cells/well obtained from the liner portion of the standard curve related to the optimum volume. Discussion: The optimal cell density and volume of cell culture medium for hepa1-6 cell line were found. In addition, a simple practical protocol was suggested to find the optimal factors mentioned for the adherent cell line in MTT assay.

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Section: Discussionmentioning

confidence: 99%

Determining Optimal Cell Density and Culture Medium Volume simultaneously in MTT Cell Proliferation Assay for Adherent Cancer Cell Lines

Moradi¹

2018

Helix

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“…MTT cytotoxicity assay was performed on eumelanin. Slight modifications were made to the experimental method of Eren et al ., 25 as described in Song et al 19 . Eumelanin concentrations were set at 50, 100, 200, 400 and 800 mg L −1 .…”

Section: Methodsmentioning

confidence: 99%

Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression

Song,

Xing,

Yang

et al. 2023

J Sci Food Agric

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BackgroundType 2 diabetes mellitus (T2DM) is a prevalent metabolic disease that poses significant health risks due to its numerous complications. However, the effects of eumelanin on oxidative stress, hyperglycemia, and depression in diabetic mice haven't been extensively studied.ResultsOur study employed an enzymatic approach to extract eumelanin from squid ink and characterized it using spectroscopic techniques. Remarkably, eumelanin extracted with alkaline‐neutral‐flavor protease (ANF) displayed superior inhibitory activity against α‐glucosidase and α‐amylase, while enhancing glucose utilization and hepatic glycogen synthesis in human hepatocellular carcinoma cell line (HepG2) insulin resistance model. Further evaluation of ANF in a T2DM ICR mouse model demonstrated its significant potential in alleviating hyperglycemia, reducing glycosylated serum protein levels, improving glucose tolerance, and modulating total cholesterol (TC), low‐density lipoprotein (LDL) levels, as well as antioxidant indices (SOD, GSH‐Px, and MDA) at a dosage of 0.04 g·kg‐1. Additionally, ANF exhibited positive effects on energy levels and reduced immobility time in antidepressant behavioral experiments. Moreover, ANF positively influenced the density and infiltration state of renal cells, while mitigating inflammatory enlargement and deformation of liver cells, without inducing any adverse effects in mice.ConclusionOverall, these findings underscore the significant therapeutic potential of ANF in the treatment of T2DM and its associated complications. By augmenting lipid and glucose metabolism, mitigating oxidative stress, and alleviating depression, ANF emerges as a promising candidate for multifaceted intervention.This article is protected by copyright. All rights reserved.

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“…After adding 100 μl of MTT to each well, the culture was continued in a constant temperature incubator at 37℃. Four hours after the MTT solution was sucked out and the purple crystal was fully dissolved, 100 μl of MTT termination solution was added to each well (avoid light operation), 96-well plate was wrapped with tin foil and optical density (OD) value at 492 nm was measured (Eren et al, 2015). Three parallel experiments were carried out.…”

Section: Cell Culture and Mtt Cytotoxicity Assaymentioning

confidence: 99%

Optimization of extractions of eumelanin from cuttlefish ink and the hypoglycemic effects: In vitro enzyme inhibitory activity and glucose consumption in HepG2 cells

Song

Xing

Yang

et al. 2021

J. Food Process. Preserv.

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In 1,840, Swedish researcher Berzelius first used the name melanin (Roy et al., 2019). Melanin is a polymer biopigment formed by oxidative polymerization of phenols and terpenoids with sugars and proteins. Melanin, a substance ranging from dark brown to black that can protect the skin from ultraviolet rays, is a well-known component of human hair (Riley, 1997;Xiao et al., 2018). Melanin is insoluble in water, acidic solutions, and common organic solvents (d'Ischia et al., 2013), which is characteristic of natural melanin. However, eumelanin can be extracted by dissolving in 10% sodium hydroxide aqueous solution, filtering, and precipitating with dilute hydrochloric

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A mutagenicity and cytotoxicity study on Limonium effusum aqueous extracts by Allium, Ames and MTT tests

Cited by 5 publications

References 34 publications

Determining Optimal Cell Density and Culture Medium Volume simultaneously in MTT Cell Proliferation Assay for Adherent Cancer Cell Lines

Determining Optimal Cell Density and Culture Medium Volume simultaneously in MTT Cell Proliferation Assay for Adherent Cancer Cell Lines

Therapeutic potential of enzymatically extracted eumelanin from squid ink in type 2 diabetes mellitus ICR mice: Multifaceted intervention against hyperglycemia, oxidative stress and depression

Optimization of extractions of eumelanin from cuttlefish ink and the hypoglycemic effects: In vitro enzyme inhibitory activity and glucose consumption in HepG2 cells

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