A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata

Zhu, Feng; Luo, Tao; Liu, Chaoyang; Wang, Yang; Li, Zheng; Xiao, Xue; Zhang, Mingfei; Yang, Hong-Chang; Yang, Wei; Xu, Rangwei; Zeng, Yunliu; Ye, Junli; Xu, Juan; Xu, Jianguo; Larkin, Robert M.; Wang, Pengwei; Wen, Weiwei; Deng, Xiuxin; Fernie, Alisdair R.; Cheng, Yunjiang

doi:10.1093/jxb/eraa118

Cited by 50 publications

(30 citation statements)

References 60 publications

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“…There are many reports of the effects of ABA on ripening, as discussed by Zhu et al [ 157 ] and reviewed recently by Kou et al [ 158 , 159 , 160 ]. ABA is synthesised from carotenoids via the action of 9-cis-epoxycarotenoid dehydrogenase (NCED) [ 161 , 162 ].…”

Section: Ripening Is Influenced By Multiple Hormones and Lightmentioning

confidence: 99%

“…Overexpression of Sl-SAUR69 in tomato causes premature initiation of ripening, whereas its downregulation delays ripening initiation [139,142]. There are many reports of the effects of ABA on ripening, as discussed by Zhu et al [157] and reviewed recently by Kou et al [158][159][160]. ABA is synthesised from carotenoids via the action of 9-cis-epoxycarotenoid dehydrogenase (NCED) [161,162].…”

Section: Auxin Delays Ripening and Antagonises The Effects Of Ethylenementioning

confidence: 99%

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Molecular and Hormonal Mechanisms Regulating Fleshy Fruit Ripening

Chen

Grierson

2021

Cells

127

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This article focuses on the molecular and hormonal mechanisms underlying the control of fleshy fruit ripening and quality. Recent research on tomato shows that ethylene, acting through transcription factors, is responsible for the initiation of tomato ripening. Several other hormones, including abscisic acid (ABA), jasmonic acid (JA) and brassinosteroids (BR), promote ripening by upregulating ethylene biosynthesis genes in different fruits. Changes to histone marks and DNA methylation are associated with the activation of ripening genes and are necessary for ripening initiation. Light, detected by different photoreceptors and operating through ELONGATED HYPOCOTYL 5(HY5), also modulates ripening. Re-evaluation of the roles of ‘master regulators’ indicates that MADS-RIN, NAC-NOR, Nor-like1 and other MADS and NAC genes, together with ethylene, promote the full expression of genes required for further ethylene synthesis and change in colour, flavour, texture and progression of ripening. Several different types of non-coding RNAs are involved in regulating expression of ripening genes, but further clarification of their diverse mechanisms of action is required. We discuss a model that integrates the main hormonal and genetic regulatory interactions governing the ripening of tomato fruit and consider variations in ripening regulatory circuits that operate in other fruits.

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Section: Ripening Is Influenced By Multiple Hormones and Lightmentioning

confidence: 99%

Section: Auxin Delays Ripening and Antagonises The Effects Of Ethylenementioning

confidence: 99%

Molecular and Hormonal Mechanisms Regulating Fleshy Fruit Ripening

Chen

Grierson

2021

Cells

127

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“…Interestingly, recent reports suggest that the grape U-box E3 ubiquitin ligase VlPUB38 negatively regulates berry ripening in an ABA-dependent manner by degradation of abscisic-aldehyde oxidase (VlAAO), the enzyme catalyzing the last step of ABA biosynthesis ( Yu et al, 2020 ). A synergistic interaction between CrNAC036 and CrMYB68 appears to inhibit CrNCED5-mediated ABA biosynthesis at the transcription level in citrus fruit ( Zhu et al, 2020 ).…”

Section: Central Roles Of Aba In Regulation Of Non-climacteric Fruit mentioning

confidence: 99%

The Physiological and Molecular Mechanism of Abscisic Acid in Regulation of Fleshy Fruit Ripening

2021

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The ripening of fleshy fruits is coupled with the degradation of both chlorophyll and cell walls, as well as changes in the metabolism of phenylpropanoids, flavonoids, starch/sucrose, and carotenoids. These processes are controlled by phytohormones and other factors, including abscisic acid (ABA), ethylene, auxin, polyamines, sugar, and reactive oxygen species. The ripening of climacteric fruits is controlled by ethylene and non-climacteric fruit ripening is regulated mainly by ABA. Also, ABA and ethylene may interact in both types of fruit ripening. ABA concentrations in fleshy fruits are regulated in response to developmental and environmental cues and are controlled by the relative rates of ABA biosynthesis and catabolism, the former mainly via 9-cis-epoxycarotenoid dioxygenases (NCEDs) and β-glucosidases and the latter via ABA 8'-hydroxylases (CYP707As) and β-glycosyltransferases. In strawberry fruit ripening, ABA is perceived via at least two receptors, Pyrabactin resistance (PYR)/PYR-like (PYL) and putative abscisic acid receptor (ABAR), which are linked separately to the conserved signaling pathway ABA-FaPYR1-FaABIl-FaSnRK2 and the novel signaling pathway ABA-FaABAR-FaRIPK1-FaABI4. Downstream signaling components include important transcription factors, such as AREB (ABA responsive element binding protein)/ABF (ABRE binding factors ABA responsive factor), ethylene response factor (ERF), and V-myb Myeloblastosis viral oncogene homolog (MYB), as well as ripening-related genes. Finally, a comprehensive model of ABA linked to ethylene, sugar, polyamines, auxin and reactive oxygen species in the regulation of strawberry fruit ripening is proposed. Next, new integrated mechanisms, including two ABA signaling pathways, ABA and ethylene signaling pathways, and ABA/ethylene to other phytohormones are interesting and important research topics in ripening, especially in non-climacteric fruits.

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“…NAC is one of the largest gene families in plants and is involved in stress tolerance regulation 33 , plant development, and fruit ripening 16 , 34 . In banana, MaNAC1 and MaNAC2 are transcriptional repressors and negatively regulate MaERF11 to induce ethylene accumulation and fruit ripening 35 .…”

Section: Discussionmentioning

confidence: 99%

The NAC transcription factor ClNAC68 positively regulates sugar content and seed development in watermelon by repressing ClINV and ClGH3.6

Wang

Zhang

et al. 2021

Horticulture Research

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NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in fruit ripening and quality. The watermelon genome encodes 80 NAC genes, and 21 of these NAC genes are highly expressed in both the flesh and vascular tissues. Among these genes, ClNAC68 expression was significantly higher in flesh than in rind. However, the intrinsic regulatory mechanism of ClNAC68 in fruit ripening and quality is still unknown. In this study, we found that ClNAC68 is a transcriptional repressor and that the repression domain is located in the C-terminus. Knockout of ClNAC68 by the CRISPR-Cas9 system decreased the soluble solid content and sucrose accumulation in mutant flesh. Development was delayed, germination was inhibited, and the IAA content was significantly decreased in mutant seeds. Transcriptome analysis showed that the invertase gene ClINV was the only gene involved in sucrose metabolism that was upregulated in mutant flesh, and expression of the indole-3-acetic acid-amido synthetase gene ClGH3.6 in the IAA signaling pathway was also induced in mutant seeds. EMSA and dual-luciferase assays showed that ClNAC68 directly bound to the promoters of ClINV and ClGH3.6 to repress their expression. These results indicated that ClNAC68 positively regulated sugar and IAA accumulation by repressing ClINV and ClGH3.6. Our findings provide new insights into the regulatory mechanisms by which NAC transcription factors affect fruit quality and seed development.

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A NAC transcription factor and its interaction protein hinder abscisic acid biosynthesis by synergistically repressing NCED5 in Citrus reticulata

Cited by 50 publications

References 60 publications

Molecular and Hormonal Mechanisms Regulating Fleshy Fruit Ripening

Molecular and Hormonal Mechanisms Regulating Fleshy Fruit Ripening

The Physiological and Molecular Mechanism of Abscisic Acid in Regulation of Fleshy Fruit Ripening

The NAC transcription factor ClNAC68 positively regulates sugar content and seed development in watermelon by repressing ClINV and ClGH3.6

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