A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1

Li, Du-Juan; Wang, Jianping; Wang, Ronghui; Li, Yanbin; Abi-Ghanem, Daad; Berghman, Luc; Hargis, Billy M.; Lu, Huaguang

doi:10.1016/j.bios.2011.04.010

Cited by 127 publications

(84 citation statements)

References 51 publications

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“…Table 2 shows the selected examples of NAb-QCM biosensors for viruses. For instance, Li et al [93] fabricated a QCM immunosensor using magnetic nanobeads and polyclonal anti-H5 antibodies for the detection of avian influenza virus (AIV) H5N1 in agricultural, food, environmental, and clinical samples. The surface antigen hemagglutinin (HA) was deposited on QCM through self-assembled monolayer of 16-mercaptohexadecanoic acid.…”

Section: Natural Antibodies For Qcm Virus Sensorsmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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Viruses are pathogenic microorganisms that can inhabit and replicate in human bodies causing a number of widespread infectious diseases such as influenza, gastroenteritis, hepatitis, meningitis, pneumonia, acquired immune deficiency syndrome (AIDS) etc. A majority of these viral diseases are contagious and can spread from infected to healthy human beings. The most important step in the treatment of these contagious diseases and to prevent their unwanted spread is to timely detect the disease-causing viruses. Gravimetric viral diagnostics based on quartz crystal microbalance (QCM) transducers and natural or synthetic receptors are miniaturized sensing platforms that can selectively recognize and quantify harmful virus species. Herein, a review of the label-free QCM virus sensors for clinical diagnostics and point of care (POC) applications is presented with major emphasis on the nature and performance of different receptors ranging from the natural or synthetic antibodies to selective macromolecular materials such as DNA and aptamers. A performance comparison of different receptors is provided and their limitations are discussed.

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Section: Natural Antibodies For Qcm Virus Sensorsmentioning

confidence: 99%

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

et al. 2017

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“…Самыми распространенными из них яв ляются микровесы на основе к варцевого криста лла [7,8,9], а так же дат чик на основе эффекта поверхностных акустических волн в пьезокри-сталле [10]. В новых разработках так же пред-лагается использовать емкостные микроме-ханические ультразвуковые преобразователи [11] и мембранные пьезоакустические резона-торы [12].…”

Section: Mems In the Control Of Bacteria And Virusesunclassified

“…N u m e r o u s p u b l i c a t i o n s describe the use of various resonant sensors for virus detection. The most common sensors are quartz crystal based microbalances [7,8,9] and sensors based on the effect of surface acoustic waves in a piezoelectric crystal [10]. In new developments, it is also proposed to use capacit ive m ic romecha n ica l u lt rasonic transducers [11] and membrane piezo-acoustic resonators [12].…”

Section: анализ вирусных частиц с применением пьезокерамического кантmentioning

confidence: 99%

Detection of viruses using piezoelectric cantilevers

Kiselev¹,

Gorelkin²,

Erofeev³

et al. 2015

NanoRus

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Быстрый прямой анализ вируса гриппа А в жидкой среде может выполняться с помощью пьезоэлектрического кантилевера с рецептором, модифицированным гликополимером, который содержит сиаловые группы, специфичные к белку оболочки вируса. При помещении кантилевера в раствор вируса в аллантоисной жидкости наблюдалcя положительный сдвиг резонансной частоты продольной моды колебаний кантилевера. Положительный результат прямого измерения без использования меток был достигнут в течение 10 мин без длительной пробоподготовки, что говорит о простоте метода. Rapid direct analysis of influenza A virus in a liquid medium may be performed using a piezoelectric cantilever with a glycopolymer-modified receptor containing sialic groups that are specific to the protein of the viral envelope. When placing a cantilever in the solution of a virus in allantoic fluid, a positive shift is observed in the resonance frequency of the longitudinal vibrations of the cantilever. A directly measured positive result without any lables was achieved within 10 min without a lengthy sample preparation, which indicates the simplicity of the method. к современным сенсорным системам предъ-являются экстремально высокие требова-ния. К их к ритическ им хара к терис ти-кам мож но отнести параметр предела чув-ствительности, линейные размеры датчика и скорость ана лиза. Во всем многообразии датчиков можно выделить активно развива-ющийс я подк ласс мик роэлек т ромеха ниче-ских систем (МЭМС) -различного рода резо-наторов, наноамплитудные колебания в кото-рых возбуждаются под действием переменного электрома гнитного поля. Резонанс в так их системах напрямую зависит от их геометри-ческой топологии. По своей сути МЭМС -это маятники, пружины, струны или мембраны, имеющие собственные частоты на различных гармониках.Примером оригинального применения для получения научного результата хорошо извест-ного из часовых механизмов резонатора квар-цевой вилки может быть работа под руковод-ством Франца Гизибла [1]. Наноколебания, воз-никающие в кварцевой вилке, которая служит для за дания опорной частоты, очень приго-дились сотрудникам университета Аугсбурга. Они предложили использовать вилку в каче-стве туннельного микроскопа, прикрепив на ее конец срез платиновой проволоки. Игла из проволоки служила щупом поверхности моно-кристаллического кремния (рис.1). Благодаря высокой добротности кварцевого резонатора атомную структуру кремния уда лось разре-шить с точностью до нескольк их долей анг-стрем.

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“…One good candidate to detect milk adulteration is by using quartz crystal microbalance (QCM). As shown in many works, the QCM sensor has been proved to be a good immunosensor [15][16][17][18][19][20]. Commercial quartz crystal resonator with polystyrene coating has been used successfully and developed as a basis for QCM Immunosensor [21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

et al. 2016

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Adulteration of goat milk is usually done using cow's milk product. Cow milk is used as it is widely available and its price is cheaper compared to goat milk. This paper shows a development of candidate tools for milk adulteration using cow milk. A quartz crystal microbalance immunosensor was developed using commercial crystal resonator and polyclonal antibody specific to cow milk protein. A specific protein at 208 KDa is found only in cow milk and does not exist in goat milk. The existence of this protein can be used as an indicator of cow milk content in a target solution. To detect the PSS 208 kDa protein, antibody specific to the PSS 208 was developed. The purified antibody was immobilized on top of the sensor surface on a polystyrene layer. The fraction of the immobilized antibody on the sensor was found at 1.5% of the given antibody. Using a static reaction cell, the developed immunosensor could detect the specific cow milk protein in buffer solution. The detection limit is 1 ppm. A linear relationship between frequency change and specific protein of cow milk concentration is found from a concentration of 1 ppm to 120 ppm.

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A nanobeads amplified QCM immunosensor for the detection of avian influenza virus H5N1

Cited by 127 publications

References 51 publications

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Gravimetric Viral Diagnostics: QCM Based Biosensors for Early Detection of Viruses

Detection of viruses using piezoelectric cantilevers

Development of QCM Biosensor with Specific Cow Milk Protein Antibody for Candidate Milk Adulteration Detection

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