A nanocluster design for the construction of artificial cellulosomes

Kim, Do Myoung; Nakazawa, Hikaru; Umetsu, Mitsuo; Matsuyama, Takashi; Ishida, Nobuhiro; Ikeuchi, Akinori; Takahashi, Hitomi; Asano, Ryutaro; Kumagai, Izumi

doi:10.1039/c2cy00371f

Cited by 24 publications

(35 citation statements)

References 22 publications

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“…For this purpose, the catalytic domain of endoglucanase CelD from Clostridium thermocellum and different CBMs were biotinylated and bound to streptavidin–cadmium selenide nanoparticles as a scaffold. They showed that the artificial cellulosome built on nanoparticle scaffolds was capable of degrading cellulose [98].…”

Section: Protein Engineeringmentioning

confidence: 99%

Genetically Engineered Proteins to Improve Biomass Conversion: New Advances and Challenges for Tailoring Biocatalysts

et al. 2019

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Protein engineering emerged as a powerful approach to generate more robust and efficient biocatalysts for bio-based economy applications, an alternative to ecologically toxic chemistries that rely on petroleum. On the quest for environmentally friendly technologies, sustainable and low-cost resources such as lignocellulosic plant-derived biomass are being used for the production of biofuels and fine chemicals. Since most of the enzymes used in the biorefinery industry act in suboptimal conditions, modification of their catalytic properties through protein rational design and in vitro evolution techniques allows the improvement of enzymatic parameters such as specificity, activity, efficiency, secretability, and stability, leading to better yields in the production lines. This review focuses on the current application of protein engineering techniques for improving the catalytic performance of enzymes used to break down lignocellulosic polymers. We discuss the use of both classical and modern methods reported in the literature in the last five years that allowed the boosting of biocatalysts for biomass degradation.

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Section: Protein Engineeringmentioning

confidence: 99%

Genetically Engineered Proteins to Improve Biomass Conversion: New Advances and Challenges for Tailoring Biocatalysts

et al. 2019

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“…The enzymatic activities towards CMC-Na, phosphoric acidswollen cellulose (PASC) (Kim et al, 2012), esculin (Sigma) and Avicel (Sigma) were assayed with suitable concentrations of different substrates. The enzymatic activities against pNPG (Sigma) and oNPG (Sigma) were evaluated, as described by Yang et al (2014).…”

Section: Enzyme Assaymentioning

confidence: 99%

Alkali-tolerant β-glucosidase produced by newly isolated Aspergillus fumigatus WL002 from rotten wood

Cao

Wang

et al. 2015

International Biodeterioration & Biodegradation

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“…Chimeric cellulases can be designed to heighten the activities of cellulases by tethering two different activities together [ 55 , 56 ], however they are more commonly created as components for cellulosomes or mini-cellulosomes [ 57 - 61 ]. Bacterial strains used for the expression of these components have included E. coli [ 62 - 64 ], but gram-positive Bacillus subtilis or Clostridium species are preferentially used due to their stronger secretion abilities, surface display properties and already being cellulosome-capable [ 62 , 65 ].…”

Section: Bacterial Expression Systemsmentioning

confidence: 99%

“…Mini-cellulosomes with C. thermocellum components have been produced in the industrially-used Lactococcus lactis [ 72 ] and in Corynebacterium glutamicum , which can also be used for the production of amino acid products [ 73 ]. Cellulosomal components primarily created from cellulase and hemicellulase genes originally found in C. cellulovorans and Clostridium cellulolyticum , but also from such varied sources as Aspergillus niger and Neocallimastix patriciarum , have been successfully expressed in E. coli for in vitro assembly and used either as a complete system [ 42 , 43 , 74 ], in combination with B. subtilis produced components [ 62 ] or in systems using nanoparticles as the cellulosome scaffold [ 63 , 64 ].…”

Section: Bacterial Expression Systemsmentioning

confidence: 99%

“…This is not always a simple requirement, as described above. Those enzymes to be complexed in solution require expression of the enzymes of interest, which need to contain a dockerin or other binding module, as well as the expression or presence of a chosen scaffold, such as a protein cohesin or other binding modules [ 64 , 75 , 76 ]. For the production of these heterologous proteins, native secretion mechanisms are most frequently used [ 73 ].…”

Section: Bacterial Expression Systemsmentioning

confidence: 99%

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Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

Lambertz

Garvey

Klinger

et al. 2014

Biotechnol Biofuels

167

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Second generation biofuel development is increasingly reliant on the recombinant expression of cellulases. Designing or identifying successful expression systems is thus of preeminent importance to industrial progress in the field. Recombinant production of cellulases has been performed using a wide range of expression systems in bacteria, yeasts and plants. In a number of these systems, particularly when using bacteria and plants, significant challenges have been experienced in expressing full-length proteins or proteins at high yield. Further difficulties have been encountered in designing recombinant systems for surface-display of cellulases and for use in consolidated bioprocessing in bacteria and yeast. For establishing cellulase expression in plants, various strategies are utilized to overcome problems, such as the auto-hydrolysis of developing plant cell walls. In this review, we investigate the major challenges, as well as the major advances made to date in the recombinant expression of cellulases across the commonly used bacterial, plant and yeast systems. We review some of the critical aspects to be considered for industrial-scale cellulase production.

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A nanocluster design for the construction of artificial cellulosomes

Cited by 24 publications

References 22 publications

Genetically Engineered Proteins to Improve Biomass Conversion: New Advances and Challenges for Tailoring Biocatalysts

Genetically Engineered Proteins to Improve Biomass Conversion: New Advances and Challenges for Tailoring Biocatalysts

Alkali-tolerant β-glucosidase produced by newly isolated Aspergillus fumigatus WL002 from rotten wood

Challenges and advances in the heterologous expression of cellulolytic enzymes: a review

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