A national study through a ‘Farm‐to‐fork’ Approach to determine <i>Salmonella</i> dissemination along with the Lebanese poultry production chain

Hage, Rawad El; Rayess, Youssef El; Bonifait, Laetitia; Hafi, Bassam El; Baugé, Louise; Viscogliosi, Éric; Hamzé, Mouïn; Mathieu, Florence; Matar, Ghassan M.; Chemaly, Marianne

doi:10.1111/zph.12939

Cited by 6 publications

(10 citation statements)

References 43 publications

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“…in live chickens at the time of slaughtering differs across countries. A recent study in Egypt ( Elsayed et al, 2024 ) revealed rates comparable to those in Jordan, with a range of 12 to 17%, while a study in Lebanon ( El Hage et al, 2022 ) revealed rates as high as 31.4%. In Europe, the prevalence of Salmonella spp.…”

Section: Discussionmentioning

confidence: 92%

“…In the European Union (Authority, 2017) , only 0.3% of Salmonella spp. were detected, whereas 31% were detected in Lebanon ( El Hage et al, 2022 ), 5% in Ecuador ( Vinueza-Burgos et al, 2019 ) and 11% in Brazil ( Herman et al, 2003 ). This low prevalence may be attributed to the sanitary and biosecurity conditions in the hatchery, which contribute to the low prevalence in parent stocks.…”

Section: Discussionmentioning

confidence: 99%

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Occurrence, virulence, and resistance genes in Salmonella enterica isolated from an integrated poultry company in Jordan

Gharaibeh,

Lafi,

Allah

et al. 2024

Poultry Science

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Occurrence, virulence, and resistance genes in Salmonella enterica isolated from an integrated poultry company in Jordan

Gharaibeh,

Lafi,

Allah

et al. 2024

Poultry Science

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“…The antagonistic activity and co-aggregation ability of the lactobacilli strains were tested on three native avian Salmonella strains isolated from our previous study [ 4 , 49 ]. S .…”

Section: Methodsmentioning

confidence: 99%

“…In developing countries, a high prevalence of Salmonella has been recorded, ranging from 13% to 39% in South America, estimated at 35% in Africa, and ranging from 35% to 50% in Asia [ 3 ]. In Lebanon, according to our recent study, the percentage of contamination of poultry meat at the retail level (supermarkets and restaurants) was estimated at 22.4% [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

The Detection of Potential Native Probiotics Lactobacillus spp. against Salmonella Enteritidis, Salmonella Infantis and Salmonella Kentucky ST198 of Lebanese Chicken Origin

et al. 2022

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Salmonella continues to be a major threat to public health, especially with respect to strains from a poultry origin. In recent years, an increasing trend of antimicrobial resistance (AMR) in Salmonella spp. was observed due to the misuse of antibiotics. Among the approaches advised for overcoming AMR, probiotics from the Lactobacillus genus have increasingly been considered for use as effective prophylactic and therapeutic agents belonging to the indigenous microbiota. In this study, we isolated lactobacilli from the ilea and ceca of hens and broilers in order to evaluate their potential probiotic properties. Four species were identified as Limosilactobacillus reuteri (n = 22, 45.8%), Ligilactobacillus salivarius (n = 20, 41.6%), Limosilactobacillus fermentum (n = 2, 4.2%) and Lactobacillus crispatus (n = 1, 2%), while three other isolates (n = 3, 6.25%) were non-typable. Eight isolates, including Ligilactobacillus salivarius (n = 4), Limosilactobacillus reuteri (n = 2), L. crispatus (n = 1) and Lactobacillus spp. (n = 1) were chosen on the basis of their cell surface hydrophobicity and auto/co-aggregation ability for further adhesion assays using the adenocarcinoma cell line Caco-2. The adhesion rate of these strains varied from 0.53 to 10.78%. Ligilactobacillus salivarius A30/i26 and 16/c6 and Limosilactobacillus reuteri 1/c24 showed the highest adhesion capacity, and were assessed for their ability to compete in and exclude the adhesion of Salmonella to the Caco-2 cells. Interestingly, Ligilactobacillus salivarius 16/c6 was shown to significantly exclude the adhesion of the three Salmonella serotypes, S. Enteritidis, S. Infantis and S. Kentucky ST 198, to Caco-2 cells. The results of the liquid co-culture assays revealed a complete inhibition of the growth of Salmonella after 24 h. Consequently, the indigenous Ligilactobacillus salivarius 16/c6 strain shows promising potential for use as a preventive probiotic added directly to the diet for the control of the colonization of Salmonella spp. in poultry.

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“…Based on the White-Kauffmann-Le Minor method, over 2,650 distinct serotypes of Salmonella have been identified by their distinctive combinations of somatic (O) and flagellar (H) antigens (1,2). The infection of Salmonella enterica, like S. Enteritidis, S. Typhimurium, S. Infantis and S. Kentucky, in poultry and poultry products is on the rise (3,4). Fowls are the specific host of Salmonella enterica serovar Gallinarum biovars Pullorum and Gallinarum.…”

Section: Introductionmentioning

confidence: 99%

Accurate identification and discrimination of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum by a multiplex PCR based on the new genes of torT and I137_14430

Tan

Xiong

et al. 2023

Front. Vet. Sci.

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Most cases of chicken salmonellosis are caused by Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum, which lead to a significant morbidity and fatality rate. Although the conventional Kaufmann-White scheme is the reliable method for the serotyping of Salmonella, it does not distinguish between closely related biotypes like S. Pullorum and S. Gallinarum. Herein, we conducted a single one-step multiplex PCR assay that can identify and distinguish between S. Pullorum and S. Gallinarum in an accurate manner. This PCR method was based on three genes, including torT for S. Pullorum identification, I137_14430 for S. Gallinarum identification, and stn as the genus-level reference gene for Salmonella. By comparing S. Pullorum to S. Gallinarum and other serovars of Salmonella, in silico study revealed that only the former has a deletion of 126 bp-region in the carboxyl terminus of torT. The I137_14430 gene does not exist in S. Gallinarum. However, it is present in all other Salmonella serotypes. The multiplex PCR approach utilizes unique sets of primers that are intended to specifically target these three different genes. The established PCR method was capable of distinguishing between the biovars Pullorum and Gallinarum from the 29 distinct Salmonella serotypes as well as the 50 distinct pathogens that are not Salmonella, showing excellent specificity and exclusivity. The minimal amount of bacterial cells required for PCR detection was 100 CFU, while the lowest level of genomic DNA required was 27.5 pg/μL for both S. Pullorum and S. Gallinarum. After being implemented on the clinical Salmonella isolates collected from a poultry farm, the PCR test was capable of distinguishing the two biovars Pullorum and Gallinarum from the other Salmonella strains. The findings of the PCR assay were in line with those of the traditional serotyping and biochemical identification methods. This new multiplex PCR could be used as a novel tool to reinforce the clinical diagnosis and differentiation of S. Pullorum and S. Gallinarum, particularly in high-throughput screening situations, providing the opportunity for early screening of infections and, as a result, more effective management of the illness among flocks.

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A national study through a ‘Farm‐to‐fork’ Approach to determine Salmonella dissemination along with the Lebanese poultry production chain

Cited by 6 publications

References 43 publications

Occurrence, virulence, and resistance genes in Salmonella enterica isolated from an integrated poultry company in Jordan

Occurrence, virulence, and resistance genes in Salmonella enterica isolated from an integrated poultry company in Jordan

The Detection of Potential Native Probiotics Lactobacillus spp. against Salmonella Enteritidis, Salmonella Infantis and Salmonella Kentucky ST198 of Lebanese Chicken Origin

Accurate identification and discrimination of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum by a multiplex PCR based on the new genes of torT and I137_14430

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