A Near‐Infrared‐Fluorescence‐Quenched Gold‐Nanoparticle Imaging Probe for In Vivo Drug Screening and Protease Activity Determination

Lee, Seulki; Joon, Eui; Park, Kyeongsoon; Lee, Seung Young; Hong, Jin Ki; Sun, In Cheol; Kim, Sang Yoon; Choi, Kuiwon; Kwon, Ick Chan; Kim, Kwangmeyung; Ahn, Cheol Hee

doi:10.1002/anie.200705240

Cited by 311 publications

(132 citation statements)

References 26 publications

(3 reference statements)

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“…A nearinfrared-fluorescence (NIRF)-quenched gold-nanoparticle imaging probe for in vivo drug screening and protease activity determination has been reported by Lee et al [44]. The probe is used to sense matrix metalloprotease (MMP) activity or their inhibitors which are mainly involved in cancer, inflammation and vascular disease.…”

Section: In Vivo Tumor Imagingmentioning

confidence: 99%

Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications

Huang

El‐Sayed

2010

Metal‐Enhanced Fluorescence

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Gold nanoparticles have generated widespread interests for centuries. They are optically active in multiple modalities useful for biomédical imaging including fluorescence, absorption, scattering and Raman spectroscopy. In fluorescence, gold can serve as a fluorescent probe under certain conditions, or modify the signal of an adjacent fluorophore. The effect of gold nanoparticles on fluorescence spectroscopy depends on the stimulating light source, the relation of gold nanoparticle to an adjacent fluorohpore and the surrounding medium. For instance, gold particles can enhance or quench fluorescence of adjacent fluorophores or can quench fluorophores to which they are covalently bound.To better comprehend how gold nanoparticles can demonstrate multiple effects with a range of potential biomédical applications, an understanding of the particle-light interaction is necessary. When stimulated by the electromagnetic field of the light, the free electrons of the metal nanoparticle undergo a collective coherent oscillation on the surface of the ionic metallic lattice (Figure 20.1). Figure 20.1: Surface plasmon resonance of gold nanoparticles.This collective oscillations of the free electrons confined on the particle surface is called surface plasmon. The surface plasmon is resonant at a specific frequency of the incident light called surface plasmon resonance (SPR). This optical behavior of plasmonic nanoparticles can be explained by Mie theory [1] which involves the salvation of the maxwell's equation for an electromagnetic light wave interacting with a small sphere. For nanoparticles much smaller than the wavelength of light (< 20nm), only the dipole oscillation contributes significantly to the extinction cross section and thus Mie's theory is reduced to the following equation:

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Section: In Vivo Tumor Imagingmentioning

confidence: 99%

Fluorescent Quenching Gold Nanoparticles: Potential Biomedical Applications

Huang

El‐Sayed

2010

Metal‐Enhanced Fluorescence

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“…The limitations of FRET can be overcome with a dynamic molecular method based on the distance-dependent plasmon coupling of metal nanoparticles. Recently several groups including ours [14,[18][19][20][21][22][23][24][25][26][27][28][35][36][37][38][39][40][41] have reported that nanoparticle surface-energy transfer (NSET) is a technique capable of measuring distances nearly twice as far as FRET in which energy transfer from a donor molecule to a nanoparticle surface follows a predictable distance dependence. Like FRET, [44,45] the interaction for NSET is dipole-dipole in nature, but is geometrically different beAbstract: We report size-and distancedependent surface-energy transfer (SET) properties of gold nanoparticles for recognizing hepatitis C virus (HCV) RNA sequence sensitively and selectively (single-base mutations) in a homogeneous format.…”

Section: Introductionmentioning

confidence: 99%

Size‐ and Distance‐Dependent Nanoparticle Surface‐Energy Transfer (NSET) Method for Selective Sensing of Hepatitis C Virus RNA

Griffin

Singh

Senapati

et al. 2008

Chemistry A European J

196

255

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We report size- and distance-dependent surface-energy transfer (SET) properties of gold nanoparticles for recognizing hepatitis C virus (HCV) RNA sequence sensitively and selectively (single-base mutations) in a homogeneous format. We have demonstrated that quenching efficiency increases by three orders of magnitude, as the particle size increases from 5 to 70 nm. Due to this extraordinarily high K(SV), nanoparticle SET (NSET) detection limit can be as low as 300 fM concentration of RNA, depending on the size of gold nanoparticle. We have shown that the distance-dependent quenching efficiency is highly dependent on the particle size and the distance at which the energy-transfer efficiency is 50 %, ranges all the way from 8 nm, which is very close to the accessible distance of conventional Förster resonance energy transfer (FRET), to about 40 nm by choosing gold nanoparticles of different diameters. Our result points out that dipole-to-metal-particle energy transfer and NSET models provide a better description of the distance dependence of the quenching efficiencies for 8 nm gold nanoparticle, but agreement is poor for 40 and 70 nm gold nanoparticles, for which the measured values were always larger than the predicted ones.

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“…For the control group, MMP inhibitor III (EMD Biosciences), a broad-spectrum inhibitor of various MMPs, was injected into the tumor 30 min prior to the probe injection. [23] As shown in Figure 3, without MMP inhibitor the probes demonstrated an instant response upon injection, and the activity developed steadily in the first 2 hours. On the contrary, the control group showed dramatically decreased intensities at the tumor area at all the time points, thus confirming that the activation was specifically governed by MMPs.…”

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confidence: 88%

“…[12][13][14][15] Such an implication has made MMP a good tumor marker and a common target in the design of activatable probes. [16][17][18][19] We and others have previously developed activatable probes, based on either peptide, [16,19,20] polymer, [17,21,22] or inorganic nanoparticles, [23,24] of MMP targeting specificity. On the other hand, the use of protein as a building block to construct MMP-activatable probes has, to the best of our knowledge, never been reported.…”

mentioning

confidence: 99%

“…The core peptide sequence, Pro-Leu-Gly-Val-Arg (PLGVR), has proven selectivity for multiple types of MMPs (such as MMP-2, -9, -13), which we validated in our previous investigations. [23] We assumed that the conglomeration of both dyes and quenchers on the protein surface will induce a quenched state to the overall nanostructure. Subsequently, when the probes are exposed to an MMP-rich environment, the PLGVR substrate will be cut off, thus leading to the release of Cy5.5 and the restoration of fluorescence activity (Scheme 1).…”

mentioning

confidence: 99%

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