A Network Pharmacology Approach to Explore the Potential Mechanisms of Yifei Sanjie Formula in Treating Pulmonary Fibrosis

Qiao, Bo; Wu, Yueying; Li, Xiaoya; Xu, Zhenyuan; Duan, Weigang; Hu, Yanan; Jia, Wenqing; Fan, Qiuyang; Huang, Xing

doi:10.1155/2020/8887017

Cited by 10 publications

(5 citation statements)

References 36 publications

(19 reference statements)

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“…Previous research has found that YFSJ reduces skeletal muscle injury and inhibits tumor growth in mice with lung cancer 11 . Moreover, it interferes with cell autophagy, immune, and inflammatory responses through multiple targets and pathways 9,44 . In line with the findings of these studies, we found that the use of YFSJ in lung cancer suppressed colony growth, migration, and invasion, while promoting apoptosis.…”

Section: Discussionsupporting

confidence: 87%

“…11 Moreover, it interferes with cell autophagy, immune, and inflammatory responses through multiple targets and pathways. 9,44 In line with the findings of these studies, we found that the use of YFSJ in lung cancer suppressed colony growth, migration, and invasion, while promoting apoptosis. Nonetheless, the pharmacological mechanisms of YFSJ in lung cancer remain poorly understood.…”

Section: Discussionsupporting

confidence: 87%

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Yifei Sanjie formula alleviates lung cancer progression via regulating PRMT6‐YBX1‐CDC25A axis

Tang,

Yin,

Chen

et al. 2024

Environmental Toxicology

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Lung cancer (LC) is the most prevalent cancer type, with a high mortality rate worldwide. The current treatment options for LC have not been particularly successful in improving patient outcomes. Yifei Sanjie (YFSJ), a well‐applicated traditional Chinese medicine formula, is widely used to treat pulmonary diseases, especially LC, yet little is known about its molecular mechanisms. This study was conducted to explore the molecular mechanism by which YFSJ ameliorated LC progression. The A549, NCI‐H1975, and Calu‐3 cells were treated with the YFSJ formula and observed for colony number, apoptosis, migration, and invasion properties recorded via corresponding assays. The PRMT6‐YBX1‐CDC25A axis was tested and verified through luciferase reporter, RNA immunoprecipitation, and chromatin immunoprecipitation assays and rescue experiments. Our results demonstrated that YFSJ ameliorated LC cell malignant behaviors by increasing apoptosis and suppressing proliferation, migration, and invasion processes. We also noticed that the xenograft mouse model treated with YFSJ significantly reduced tumor growth compared with the control untreated group in vivo. Mechanistically, it was found that YFSJ suppressed the expression of PRMT6, YBX1, and CDC25A, while the knockdown of these proteins significantly inhibited colony growth, migration, and invasion, and boosted apoptosis in LC cells. In summary, our results suggest that YFSJ alleviates LC progression via the PRMT6‐YBX1‐CDC25A axis, confirming its efficacy in clinical use. The findings of our study provide a new regulatory network for LC growth and metastasis, which could shed new insights into pulmonary medical research.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionsupporting

confidence: 87%

Yifei Sanjie formula alleviates lung cancer progression via regulating PRMT6‐YBX1‐CDC25A axis

Tang,

Yin,

Chen

et al. 2024

Environmental Toxicology

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“…During inflammation, I κ B phosphorylation leads to NF- κ B dissociation and activation followed by its translocation to the nucleus, where it promotes ECM generation by modulating gene expression. Previous network pharmacology and experimental pharmacology [ 52 , 53 ] studies have illustrated that the TLR4/NF- κ B or PI3K/AKT signaling pathway can be inhibited by gut microbial-derived metabolites and can exert anti-RIF and anti-inflammatory effects [ 54 , 55 ]. Here, the mRNAs and proteins of AKT, PI3K, TLR4, and NF- κ B in UUO rat kidneys showed reduced expression in response to DHHQD.…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis

Luo

Chen

Cheng

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Dahuang and Huangqi are the most frequently prescribed treatment methods for chronic kidney disease in China. Our study aimed to clarify the pharmacological mechanism of action of Dahuang-Huangqi decoction (DHHQD) in renal interstitial fibrosis (RIF). The intersection of genes targeted by DHHQD active ingredients and RIF target genes was searched using network pharmacology to build a chemical ingredient and disease target network. For in vivo analysis, Sprague–Dawley rats with unilateral urethral obstruction (UUO) were administered DHHQD, and their kidney function-related indicators and pathological indices were determined. The expression of core targets was quantified using real-time polymerase chain reaction and western blotting. A total of 139 common targets for DHHQD and RIF in chronic kidney disease were detected. Compared with the untreated UUO rats, the DHHQD-treated rats showed reductions in the following: blood urea nitrogen and serum creatinine levels, kidney tubular atrophy and necrosis, interstitial fibrosis, hyperplasia and abnormal deposition of extracellular matrix, and microstructural changes in the mesangial matrix and glomerular basement membrane. DHHQD treatment significantly regulated the levels of renal core proteins, such as eNOS, IL-6, EGFR, and VEGF and reduced the mRNA and protein expression of the core targets involved in inflammation pathways, such as PI3K/AKT and TLR4/NF-κB. DHHQD treatment ameliorated the severity of RIF by potentially regulating the AKT/PI3K and TLR4/NF-κB signaling pathways. Our study findings provide insights into the mechanisms associated with DHHQD action and essential data for future research.

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“…ch/) is a chemical target prediction platform (38). Metascape (http://metascape.org/) is used for gene function analysis (39). Cytoscape 3.2.1 is a platform-independent open-source software for visualizing networks and combining them with various types of attribute data (40).…”

Section: Methodsmentioning

confidence: 99%

Evidence‑based complementary and alternative medicine bioinformatics approach through network pharmacology and molecular docking to determine the molecular mechanisms of Erjing pill in Alzheimer's disease

et al. 2021

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Erjing pill, a Traditional Chinese Medicine (TCM) formulation composed of Polygonatum sibiricum and Lycium chinense, has an important role in the treatment of Alzheimer's disease (AD). However, the underlying mechanisms of the action of Erjing pill in AD have remained elusive. In the present study, the key ingredients of Erjing pill were investigated and the active components and their mechanisms of action on AD were analyzed based on networks pharmacology. By using the TCM and TCM Systems Pharmacology and databases, the components of Erjing pill were screened and the data were captured using Discovery Studio. The SwissTarget webserver database was used to predict the potential protein targets of Erjing pill components for pathologies related to AD. The data were further analyzed with the disease targets of AD based on analysis of the Online Mendelian Inheritance in Man, DiGSeE and Therapeutic Target Database. Subsequent analysis of mechanistic pathways of the screened components and protein targets allowed us to construct a network by using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes, which revealed potential molecular mechanisms of Erjing pill against AD. Finally, the protective effect of three active components on neurons was verified using an in vitro injury model of PC12 cells induced by Aβ 25-35 . The results indicated that 65 bioactive components of Erjing pill, including lauric acid and zederone, and 6 targets, including acetylcholinesterase, butylcholinesterase and amyloid protein precursor, were closely associated with the prevention and treatment of AD. The molecular components of Erjing pill were indicated to be involved in various biological signaling processes, mainly in synaptic signal transmission, transsynaptic signal transmission and chemical synaptic transmission. Furthermore, related pathways targeted by Erjing pill in AD included the regulation of neuroactive ligand-receptor interactions, the PI3K-Akt signaling pathway, serotoninergic synapses, calcium signaling pathways and dopaminergic synapses. A cell viability assay indicated that the compounds (polygonatine A, polygonatine C and 4' ,5-dihydroxyflavone) assessed were able to significantly improve the survival rate and increase the Ca 2+ level in a PC12 cell model of AD induced by amyloid-β 25-35 . The present study revealed that the mechanisms of action of Erjing pill to prevent and treat AD included a multicompound, multitarget and multipathway regulatory network.

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A Network Pharmacology Approach to Explore the Potential Mechanisms of Yifei Sanjie Formula in Treating Pulmonary Fibrosis

Cited by 10 publications

References 36 publications

Yifei Sanjie formula alleviates lung cancer progression via regulating PRMT6‐YBX1‐CDC25A axis

Yifei Sanjie formula alleviates lung cancer progression via regulating PRMT6‐YBX1‐CDC25A axis

Network Pharmacology and In Vivo Analysis of Dahuang-Huangqi Decoction Effectiveness in Alleviating Renal Interstitial Fibrosis

Evidence‑based complementary and alternative medicine bioinformatics approach through network pharmacology and molecular docking to determine the molecular mechanisms of Erjing pill in Alzheimer's disease

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