A network pharmacology-based approach to explore potential targets of Caesalpinia pulcherima: an updated prototype in drug discovery

Abstract

Caesalpinia pulcherima (CP) is a traditional herb used for the treatment of asthma, bronchitis, cancer, anti-bacterial, anti-fungal and as abortifacient. In the present study, bioactive components and potential targets in the treatment of breast cancer validated through in silico, in vitro and in vivo approach. The results for the analysis were as among 29 components, only four components were found active for further study which proved the use of CP as a multi-target herb for betterment of clinical uses. The results found by PPI states that our network has significant interactions which include the ESR-1, ESR-2, ESRRA, MET, VEGF, FGF, PI3K, PDK-1, MAPK, PLK-1, NEK-2, and GRK. Compound-target network involves 4 active compound and 150 target genes which elucidate the mechanisms of drug action in breast cancer treatment. Furthermore, on the basis of the above results the important proteins were fetched for the docking study which helps in predicting the possible interaction between components and targets. The results of the western blotting showed that CP regulates ER and EGFR expression in MCF-7 cell. In addition to this animal experimentation showed that CP significantly improved immunohistological status in MNU induced carcinoma rats. Network pharmacology approach not only helps us to confirm the study of the chosen target but also gave an idea of compound-target network as well as pathways associated to the CP for treating the complex metabolic condition as breast cancer and they importance for experimental verification.

Integrative Network Pharmacology of Moringa oleifera Combined with Gemcitabine against Pancreatic Cancer

“…Traditionally, the conventional approach in drug discovery is considered as "one drug, one target, one disease" theory. In contrast, network pharmacology study has been known as "multi-drug, multi-target, multi-diseases" to explore the correlation of drugs and diseases [33]. In this present study, a network pharmacology approach was proposed to identify the potential bioactive compounds and theoretically evaluate the effect of the combination therapy of MO and GEM against pancreatic cancer.…”

Immunoregulatory mechanism studies of ginseng leaves on lung cancer based on network pharmacology and molecular docking

“…Each Node represents the target protein molecules 15 . Then, according to the network topology's features, the median values for the DC, BC, CC, EC, LAC, NC were used to analyse potential drug targets 16 . Fifteen highly connected nodes (degree > 10, BC > 16.3058, CC > 0.5402, LAC > 5.4444, NC > 6.3355) were confirmed as significantly related targets with Network Analyzer in Table 2 and Fig.…”

Plant Derived Bioactive Compounds, Their Anti-Cancer Effects and In Silico Approaches as an Alternative Target Treatment Strategy for Breast Cancer: An Updated Overview

“…Among the major compounds of these cultivars, rutin has shown anticancer activity against MCF-7 cell line [ 117 ]. The systems pharmacology approach gives an idea of the target compound network as well as the signaling pathways associated with treating complex diseases like breast cancer apart from the confirmation study of the chosen target [ 118 ].…”