A new antimicrobial peptide isolated from <i>Oudneya africana</i> seeds

Hammami, Riadh; Hamida, Jeannette Ben; Vergoten, G.; Lacroix, Jean-Marie; Slomianny, Marie-Christine; Neffati, Mohamed; Fliss, Ismaı̈l

doi:10.1111/j.1348-0421.2009.00183.x

Cited by 19 publications

(11 citation statements)

References 20 publications

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“…This action can be explained by the phytochemical composition of this brassicaceae because it is very rich in saponins, flavonoids, and tannins [24; 25; 27]. Although, [26] proved that seeds of O. africana treat microbial infections by using it as proteinaceous extract against of L. monocytogenes, E. coli, B. subtilis, E. hirae, P. aeruginosa, S. aureus and C. albicans.…”

Section: Discussionmentioning

confidence: 99%

Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of Wadi Righ region

2017

SDRP-JPS

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Background: This wor k deals with the in vitro study of the antifungal effect of aqueous extr acts of six medicinal plants (Zygophyllum album, Euphorbia guyoniana, A triplex halimus, Oudneya africana, and Matricaria pubescens) in Wadi Righ region, at their inhibition of the mycelial growth of a tomato telluric fungus (Fusarium oxysporum f. sp. radicis lycopersici). Methods: The study of the antifungal activity of natur al extr acts was car r ied out accor ding to the poisoned Food technique defined by Grover and Moore (1962). Results: The in vitro study of the antifungal pr oper ties shows that all the aqueous extr acts of plant species tested inhibit the mycelial growth of this phytopathogenic agent except that of Moltkiopsis ciliata. In fact, the aqueous extract of Z. album was found to be a very inhibitory solution because it induced an inhibition rate of 75.29% against the Fusarium wilt of tomato after 8 days. Conclusion: the r esults indicate that the aqueous extr acts studied can be consider ed as a ver y pr omising agent for the plant protection industry.

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Section: Discussionmentioning

confidence: 99%

Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of Wadi Righ region

2017

SDRP-JPS

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“…It is known that PAMP have generally a relatively small size: ranging from 0.876 kDa to 8.864 kDa for Cn-AMP1 and Vicilin-like 2c-3 peptides from Cocos nucifera and Macadamia integrifolia, respectively (Mandal et al, 2009;Marcus et al, 1999). Peptides weighing less than 2.5 kDa were purified from the seeds of a south Tunisia originated plant, Oudneya africana, and found to be active against L. monocytogenes, E. coli, Bacillus subtilis, Enterococcus hirae, P. aeruginosa, S. aureus and Candida albicans (Hammami et al, 2009b). In the final chromatography step, the A. herba alba AS-P was fractioned by reverse phase HPLC and all fractions were tested for antibacterial activity against L. monocytogenes.…”

Section: Discussionmentioning

confidence: 99%

Peptides extracted from <i>Artemisia herba alba</i> have antimicrobial activity against foodborne pathogenic gram-positive bacteria

Sinda¹,

Lazhar²,

Jeridi³

et al. 2015

Afr. J. Trad. Compl. Alt. Med.

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Background: Artemisia herba alba, classified into the family of Asteraceae, is an aromatic herb that is traditionally used as a purgative and antipyretic folk medicine by rural people of south Tunisia. This study reports the first identification of antimicrobial peptides from this medicinal plant that inhibited the growth of several food-borne pathogenic bacteria. Materials and methods:The extraction and purification of peptidic agents from Artemisia herba alba, have been performed using precipitation by ammonium sulfate of a phosphate buffer crude extract obtained from the plant leaves, followed by reverse-phase HPLC on a C18 column. The mass of the peptides was estimated by SDS-PAGE electrophoresis, followed by a gel overlay assay and ultra-filtration through a 5 kDa cut-off membrane. Fractions from every purification steps were sampled and assayed for activity towards different food-borne bacterial strains pathogenic and non pathogenic to humans. Results: The phosphate buffer crude extract, as well as its ammonium sulfate precipitate, designated AS-P, inhibited the growth of Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus sensu stricto and the new approved species Bacillus cytotoxicus. AS-P MICs (minimum inhibitory concentrations) ranged from 0.241 to 3.8 mg/ml proteins for L. monocytogenes and B. cereus sensu stricto (strains ATCC10987 and IP5832), respectively. The bioactive AS-P molecules were stable up to 10 minutes heating at 120°C and they resisted organic solvent effects. Antimicrobial activity of A. herba alba AS-P decreased to 40 and 60% after proteolytic treatment with trypsin and proteinase K, respectively, suggesting peptides being responsible for the A. herba alba AS-P activity. The mass of antibacterial A. herba alba peptides was estimated below 5 kDa. Two AS-P fractions, eluted at 40 and 37% acetonitrile, showed antibacterial activity when assayed against L. monocytogenes. Conclusion: A. herba alba could make a new source of novel natural anti-infective agents that could be used in food bio-preservation as natural additives or in human infectious disease treatments against multi-drug resistant pathogens.

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“…The Minimum inhibitory concentration (MIC) assays were performed in sterile 96-well microplates (Costar 3799, Corning Incorporated, United Kingdom) [25][26][27]. In each well containing 190 μl of Műller-Hinton medium were added of 50 μl with various concentrations of AS-P (resulting from twofold serial dilutions of an initial 28.92 mg/ml protein set) and 10 μl of the strain tested prediluted in Muller-Hinton medium for a final bacterial load at 2-8 × 10 4 CFU/ml.…”

Section: Antibacterial Activity Assaysmentioning

confidence: 99%

“…In addition to the previously described components, A. herba-alba contains antimicrobial peptides. The first identification of which was made by [26]. These PAMs inhibited the growth of Listeria monocytogenes, Staphylococcus aureus, Bacillus cereus and the new approved species Bacillus cytotoxicus.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Antimicrobial Effect of the Artemisia herba-alba Aqueous Extract as a Preservative in Algerian Traditional Fresh Cheese

Adoui¹,

Aziza²,

Boughellout³

et al. 2018

J Food Microbiol Saf Hyg

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The present work was carried out in order to study the antimicrobial activity of A. herba-alba extracts and their application as food preservative. A crude extract was prepared by steeping of the dry leaves of A. herba-alba in phosphate buffer. In order to obtain an enriched fraction in active molecules, a series of ammonium sulfate precipitation (ASP) was carried out. Extracts showed antibacterial activity against E. feacalis, M. leteus and L. monocytogenes strains. The best activity is noted for the precipitate obtained at ammonium sulfate precipitation of 60% (ASP60), with inhibition zones of 23.67 ± 0.44 mm for the E. feacalis and M. leteus strains and 18.00 ± 0.67 mm for L. monocytogenes. This extract shows a MIC (minimum inhibitory concentration) of 0.23 and 0.9 mg/ml for E. feacalis and L. monocytogenes, respectively. The application of ASP60 on "Takammérite", traditional fresh cheese as preservative, cause a significant slowdown in microbial growth under refrigeration during 15 days of storage.

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A new antimicrobial peptide isolated from Oudneya africana seeds

Cited by 19 publications

References 20 publications

Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of Wadi Righ region

Biological control of Fusarium oxysporum f. sp. radicis lycopersici by using aqueous extracts of medicinal plants of Wadi Righ region

Peptides extracted from <i>Artemisia herba alba</i> have antimicrobial activity against foodborne pathogenic gram-positive bacteria

Assessment of Antimicrobial Effect of the Artemisia herba-alba Aqueous Extract as a Preservative in Algerian Traditional Fresh Cheese

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