A new apparatus for continuous cultivation of bacterial plaque on solid surfaces and human dental enamel

Noorda, Willem D; Purdell-Lewis, D.J.; Koning, W. de; Montfort, A.M.A.P. van; Weerkamp, Ah

doi:10.1111/j.1365-2672.1985.tb01712.x

Cited by 13 publications

(15 citation statements)

References 19 publications

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“…However, the possibility for manipulation of the test specimens during operation or their number per turn has been considered to be very limited for most devices described in the literature or they do not allow for the use of whole tooth specimens [Fontana et al, 2000;Noorda et al, 1985;Tang et al, 2003;Deng and ten Cate, 2004]. According to our knowledge, no reports regarding caries development at marginal gaps of fissure sealants in artificial mouth models are available in the literature written in English.…”

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confidence: 79%

“…Therefore, ventilation of the reaction chamber with a defined gas seems to be a logical consequence. Because the oral cavity is not an anaerobic environment and S. mutans biofilms have been successfully cultivated in artificial mouth models either under aerobic [Dibdin et al, 1976;Noorda et al, 1985] or anaerobic [Schiffner, 1999] conditions, we used commercially available synthetic air with 5% CO 2 in our model.…”

Section: Discussionmentioning

confidence: 99%

“…Only few reports describe the development of secondary carious lesions [Derand et al, 1991;Fontana et al, 1996;Gilmour et al, 1990;Schiffner, 1999]. Since it is widely accepted that Streptococcus mutans plays an important role in both the development of primary and secondary caries, it has been utilised in in vitro caries systems for the production of caries-like lesions [Fontana et al, 1996;Noorda et al, 1985;Schiffner, 1999;Deng and ten Cate, 2004].…”

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confidence: 99%

“…More relevant in vitro experiments on primary and secondary caries also regarding microbial and antimicrobial properties or problems resulting from insufficient marginal adaptation of restorations can be revealed by using artificial mouth models (for a review, see Tang et al [2003]). Besides complex systems developed to study the interaction between different bacterial species during bacterial biofilm formation [Shu et al, 2000;Sissons, 1997], several artificial mouth models have been established to study factors influencing the development of primary carious lesions [Dummer et al, 1982;Levine and Coulter, 1976;Noorda et al, 1985;Schiffner, 1993;Shu et al, 2000]. Only few reports describe the development of secondary carious lesions [Derand et al, 1991;Fontana et al, 1996;Gilmour et al, 1990;Schiffner, 1999].…”

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A Novel in vitro Microbial-Based Model for Studying Caries Formation – Development and Initial Testing

et al. 2005

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We report on the development and the initial testing of a new microbial-based caries model. Specimens were fixed on a rotating mount within a reaction chamber hermetically surrounded by a sterilised glove box. A cariogenic environment was obtained by inoculation with Streptococcus mutans (ATCC 25175) combined with a continuously repeating supply of sucrose solution, trypticase soy broth and artificial saliva applied by dripping. Twenty-five caries-free upper premolars were used. The mesial parts of the occlusal fissures had been sealed with a resin-based fissure sealant (test group 1). To produce marginal gaps, the distal parts had been moistened with saliva before resin application (test group 2). Five teeth served as control and were exposed to all fluids under sterile conditions before being removed from the system after 7 days. Test specimens were infected with S. mutans and were incubated for another 14 days. No unintentional contamination occurred during the 3-week period of operation. Demineralizations were evaluated by using confocal laser scanning microscopy. Only the test specimens showed clearly visible signs of biofilm formation and caries-like lesions. The mean primary lesion depth did not differ significantly between test groups. Wall lesion depths and surface areas of demineralizations underneath the fissure sealants were significantly higher in test group 2. Thus, our model allows the simultaneous production of primary and secondary caries-like enamel lesions in a considerable number of specimens and facilitates the possibility to manipulate and transfer them without necessarily terminating the experiment, opening new possibilities for in vitro caries research.

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confidence: 79%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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A Novel in vitro Microbial-Based Model for Studying Caries Formation – Development and Initial Testing

et al. 2005

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“…They concluded that 'a mixed-culture chemostat is a suitable system for studying the effects of nutrients on microbial populations; however, unlike the 'artificial mouth' system, the chemostat model does not account for the spatial arrangement o f dental plaque' since bacteria are studied in suspension and not as a biofilm on the tooth sur face. Zampatti et al [1994] used S. mutans in a mouth model based on Noorda et al [1985] to test the efficacy of anti plaque agents. Their model involved the use of bovine teeth to study plaque formation, but no attempt was made to study dental caries.…”

Section: Discussionmentioning

confidence: 99%

An in vitro Microbial Model for Studying Secondary Caries Formation

Fontana¹,

Dunipace²,

Gregory

et al. 1996

Caries Res

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Secondary caries is a major reason for the replacement of restorations. Because it is hypothesized that the development of secondary caries is closely associated with pathogenic oral bacteria, an in vitro microbial model has been developed to produce secondary carious lesions. A mixture of overnight cultures of Streptococcus mutans and Lactobacillus casei in dextrose-free trypticase soy broth, supplemented with 5% sucrose (TSBS), at 37°C was used in this model as the inoculum for the experimental groups. Uninoculated control groups were incubated with medium only. Groups of human tooth specimes restored using composite, together with their respective controls, were exposed for 7 or 12 days to circulating cycles of TSBS (30 min each, 3 times per day) and a mineral wash solution (for a total of 22.5 h per day), at 37°C. The pH of the experimental groups dropped to 4.1–4.5 during the test periods. The pH of the control groups remained at 6.8–7.0. The inoculated bacteria remained viable throughout the study. No contamination of experimental or control samples occurred. Laser scanning confocal microscopy demonstrated the development of incipient surface and wall lesions in all the specimens of experimental groups in as few as 7 days. Reproducibility of the model was confirmed in a second investigation. Therefore, it was concluded that this model can be used for studying the microbial etiology and prevention of secondary caries.

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