Potassium and sodium ions (K + and Na + ) play crucial roles in influencing plant growth and health status. Unfortunately, current strategies to determine the concentrations of such ions are destructive for the plants because it is necessary to collect/extract the sap for further analysis and produce either scattered or delayed results. Here, we introduce a new potentiometric dual microneedle sensor for nondestructive, realtime, and continuous monitoring of K + and Na + concentrations in living plants. The developed sensors show a response time <5 s, close-to-Nernstian slope (∼55 mV dec −1 ), resiliency to five insertions on the stem, good repeatability (max. %RSD = 0.3%) and reversibility (max. %RSD = 3%), appropriate continuous operation for 24 h, and linear range of responses that cover expected plant physiological levels (5−50 mM for Na + and 50−120 mM for K + ). Moreover, the accuracy was successfully investigated by comparing the results provided by the microneedle sensors to those obtained by a standard reference method (e.g., ion chromatography). Finally, we demonstrate that the developed analytical device is capable of tracking K + and Na + transportation from the hydroponic solution to the stem within 5−10 min. This research will contribute to establishing a new generation of analytical platforms for smart agriculture offering real-time information.