A new approach for treatment of deep skin infections by an ethosomal antibiotic preparation: an in vivo study

Godin, Biana; Touítou, Elka; Rubinstein, Ethan; Athamna, Abed; Athamna, Muhammad

doi:10.1093/jac/dki125

Cited by 80 publications

(36 citation statements)

References 14 publications

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“…In some cases they can be a cause of extensive morbidity and mortality [30] . S. aureus produces several biologically active products, including hemolysins, nuclease, coagulase, lipase, exotoxins, fibronectin-and collagenbinding proteins and enterotoxins.…”

Section: Discussionmentioning

confidence: 99%

“…After agar solidification, the well (7 mm in diameter) was cut from the agar to produce a total of three wells per each agar plate. For preliminary test, six concentrations of each extract (10,20,30,40, 50 and 60% v/v) were prepared in a total volume of 100 µL per well using 99.5% analytical Dimethyl Sulphoxide (DMSO) as an organic solvent. Chloramphenicol (10 µg/well) and amphotericin B (10 µg/well) were used as positive standard antibiotics.…”

Section: Agar Well Diffusion Assaymentioning

confidence: 99%

“…Skin infection of BALB/c mice with S. aureus (ATCC 25923) or a clinical isolate was conducted as described by Godin et al [30] . The assay was run on a total of forty-eight mice randomly divided into two groups.…”

Section: Infection Of Animalsmentioning

confidence: 99%

“…To quantify the bacterial numbers, samples were diluted to 1:10, plated on nutrient agar plates and incubated for 24 h at 37°C. Bacterial counts were expressed as numbers of S. aureus colony forming units per gram (cfu g −1 ) of tissue [29,30] .…”

Section: Infection Of Animalsmentioning

confidence: 99%

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In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Abu-Al-Basal¹

2009

American J. of Applied Sciences

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Problem statement:The developing microbial resistance to the existing anti-microbial agents has become a real challenge and a serious problem facing patients suffering from skin infections. Seeds of Nigella sativa have been used for a long time in folk medicine for the treatment of such infections. Production of new potent agents is urgently needed, especially for hospitals and health centers. Therefore, the anti-microbial effect of aqueous, diethyl ether, chloroform and petroleum ether extracts of the seeds against four standard microbial strains and seven clinical isolates from patients with skin wound infections were investigated. Approach: The in vitro anti-microbial effect of the extracts at a concentration of 20% on standard strains and clinical isolates was assessed and compared with standard drugs, chloramphenicol and amphotericin B using agar well diffusion assay. The in vivo anti-bacterial effect of petroleum ether extract was studied in male BALB/c mice infected subcutaneously with S. aureus (ATCC 25923) or a clinical isolate (0.1 mL from 10 9 colony forming units mL −1 suspension) and immediately treated at the infected site by subcutaneous injection of 0.1 mL of pure extract (fixed oil) or chloramphenicol or normal saline. Counts of viable bacteria present in the skin area corresponding to the infected site were determined, after 24 and 48 h of infection and treatment. Results: The aqueous extract did not show any inhibitory effect against all the tested microorganisms. The diethyl ether and chloroform extracts indicated significant inhibitory effect only against Gram-positive bacteria. However, petroleum ether extract was proved to be the most powerful one against these bacteria and also against other clinical isolates like one Gram-negative bacterium (Klebsiella pneumonia) and the yeast (Candida albicans). Moreover, the extract revealed a superior effect over the standard drug, chloramphenicol, on the clearance of subcutaneous staphylococcal infection in mice when injected at the site of infection. Counts of viable bacteria were decreased at highly significant level in mice infected with S. aureus (ATCC 25923) or a clinical isolate. Conclusion/Recommendations:The results of this study revealed clear potentiality of N. sativa fixed oil as a source for anti-microbial drugs and support its use in folk medicine for the treatment of microbial skin infections.

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Section: Discussionmentioning

confidence: 99%

Section: Agar Well Diffusion Assaymentioning

confidence: 99%

Section: Infection Of Animalsmentioning

confidence: 99%

Section: Infection Of Animalsmentioning

confidence: 99%

See 2 more Smart Citations

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Abu-Al-Basal¹

2009

American J. of Applied Sciences

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“…It is thought that cholesterol stabilizes into a bilayer when ethosomes are maintained in a gel state, and that the high concentration of ethanol in ethosomes can ensure mobility of the vesicles, and that a moderate amount of cholesterol could ensure stability. [10][11][12] The purpose of this study was to develop a sustained-release dermal patch with a stable formulation and good entrapment efficiency, release rate, and transdermal absorption.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a ligustrazine ethosome patch and its evaluation in vitro and in vivo

Liu

Liu²,

Liu³

et al. 2011

IJN

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Background The purpose of this study was to develop a transdermal ligustrazine patch containing a stable formulation and with good entrapment efficiency, release rate, and transdermal absorption. Methods Ligustrazine ethosomes were prepared by ethanol injection-sonication, with entrapment efficiency as an indicator. Using acrylic resin as the primary constituent, the ligustrazine ethosome patch was prepared by adding succinic acid as a crosslinking agent and triethyl citrate as a plasticizer. In vitro release and transdermal permeation studies were carried out. Finally, a pharmacokinetic study was carried out in rats to explore relative bioavailability. The formulations of ligustrazine ethosome were 1% (w/v) phospholipid, 0.4% (w/v) cholesterol, and 45% (v/v) ethanol. Results Ligustrazine ethosomes were obtained with an average particle size of 78.71 ± 1.23 nm and an average entrapment efficiency of 86.42% ± 1.50%. In vitro transdermal testing of the ligustrazine ethosome patches showed that the cumulative 24-hour amount of ligustrazine was up to 183 ± 18 μg/cm 2 . The pharmacokinetic results revealed that the relative bioavailability was 209.45%. Conclusion Compared with conventional ligustrazine administration, ligustrazine ethosome patches could promote better drug absorption and increase bioavailability. This study demonstrates that the transdermal action of the ligustrazine ethosome patch was comparatively good.

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Nanodelivery of Food Polyphenols for Nutraceutical Applications

Yusuf,

Ambawat,

Dokhale

et al. 2023

Polyphenols

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A new approach for treatment of deep skin infections by an ethosomal antibiotic preparation: an in vivo study

Abstract: Therapy with ethosomal erythromycin applied to the skin of S. aureus-infected mice was as effective as systemically administered erythromycin, suggesting a new possibility to treat deep dermal infections by local application of antibiotic in ethosomal carrier.

Cited by 80 publications

References 14 publications

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Preparation of a ligustrazine ethosome patch and its evaluation in vitro and in vivo

Nanodelivery of Food Polyphenols for Nutraceutical Applications

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