2013
DOI: 10.1016/j.radphyschem.2013.03.037
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A new approach to studying the effects of ionising radiation on single cells using FTIR synchrotron microspectroscopy

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Cited by 27 publications
(34 citation statements)
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“…The spectrum of each cell was acquired with an aperture of 12 μm ×12 μm. The analysis of the 3000-2800 cm −1 spectral range, that provided information about the state-of-order of the hydrocarbon tails in lipids and acyl chains in general, pinpointed a greater bands width at 2852 and 2925 cm −1 (relative to CH 2 symmetric and asymmetric stretching modes, respectively) in samples submitted to ionizing radiation compared to those not subjected to radiation [36].…”
Section: Ftir Microspectroscopymentioning
confidence: 98%
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“…The spectrum of each cell was acquired with an aperture of 12 μm ×12 μm. The analysis of the 3000-2800 cm −1 spectral range, that provided information about the state-of-order of the hydrocarbon tails in lipids and acyl chains in general, pinpointed a greater bands width at 2852 and 2925 cm −1 (relative to CH 2 symmetric and asymmetric stretching modes, respectively) in samples submitted to ionizing radiation compared to those not subjected to radiation [36].…”
Section: Ftir Microspectroscopymentioning
confidence: 98%
“…Its application to investigate biomedical questions took place more than 50 years ago, even if, owing to the lack of adequate technology, the potential of this technique was completely exploited only many years later [8,70]. Over the last 20 years, the coupling of IR spectrometers with visible microscopes has led to the successful use of this technique to perform imaging analysis, in which biochemical and spatial information of non-homogeneous biological samples, such as tissues and cells, are combined [23,36,41,46,49,55,72]. Fourier Transform Infrared Microspectroscopy (FTIRM) requires thin sections of sample (monolayer cell cultures or 5-10 μm thick tissues) on which IR maps can be acquired on previously selected areas.…”
Section: Introductionmentioning
confidence: 99%
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“…More importantly, the non-destructive features of biospectroscopy allow its application in vivo and in situ. Some successful microbial applications of biospectroscopy (Figure 1) include characterization of hospital isolates and rapid quantitative detection of the microbial spoilage of food products 71,77,79,91,92 . There is no doubt that biospectroscopy is a robust tool for distinguishing bacterial responses to environmental exposures due to its particular attributes of being non-destructive, non-intrusive, high throughput and label-free 69,72 .…”
Section: Applications Of Biospectroscopy In Microbial Researchmentioning
confidence: 99%
“…FTIR is a valuable metabolic fingerprinting tool owing to its abilities to characterize cellular composition [72][73][74][75][76][77][78] . In the mid-IR spectroscopy, the biochemical fingerprint region is from 1800 -900 cm 69,72,77,79,80 . These peaks can be derived as biomarkers for characterization of microbial cell types (even at subspecies level) and diagnosis of microbe-induced diseases [72][73][74][75][76][77][78] .…”
mentioning
confidence: 99%