“…Its application to investigate biomedical questions took place more than 50 years ago, even if, owing to the lack of adequate technology, the potential of this technique was completely exploited only many years later [8,70]. Over the last 20 years, the coupling of IR spectrometers with visible microscopes has led to the successful use of this technique to perform imaging analysis, in which biochemical and spatial information of non-homogeneous biological samples, such as tissues and cells, are combined [23,36,41,46,49,55,72]. Fourier Transform Infrared Microspectroscopy (FTIRM) requires thin sections of sample (monolayer cell cultures or 5-10 μm thick tissues) on which IR maps can be acquired on previously selected areas.…”