A new approach to the preeclampsia puzzle; MicroRNA-326 in CD4+ lymphocytes might be as a potential suspect

Zolfaghari, Mohammad Ali; Motavalli, Roza; Soltani‐Zangbar, Mohammad Sadegh; Parhizkar, Forough; Danaii, Shahla; Aghebati‐Maleki, Leili; Noori, Mohammad; Ahmadi, Majid; Kafil, Hossein Samadi; Jadidi‐Niaragh, Farhad; Heris, Javad Ahmadian; Mahmoodpoor, Ata; Hejazi, Mohammad Saeid; Yousefi, Mehdi

doi:10.1016/j.jri.2021.103317

Cited by 17 publications

(12 citation statements)

References 44 publications

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“…Many investigations have found that whereas Th1 cells are prominent in abortion, the presence of Th2 cells has also been observed in recurrent pregnancy loss [ 50 , 51 ]. However, the Th17/Treg balance is disturbed in particular pregnancy disorders such as preterm birth, preeclampsia, and URPL [ 38 , 52 ]. In concordance with previous studies, our results showed that the Th1/Th2 and Th17/Treg ratios considerably declined in ongoing pregnancy group.…”

Section: Discussionmentioning

confidence: 99%

“…PBMCs were incubated for 45 min at 4 °C with FITC-conjugated anti-CD4, and anti-CD127-APC, and anti-CD25-PE-conjugated antibodies (BD Biosciences, CA, USA) for Treg detection, and anti-CD56 fluorochrome-conjugated antibody was used for NK cells frequency assessment. PBMCs were stained and incubated with antibodies based on previous work [ 38 , 39 ]. The flow cytometry-based method was assessed NK cells cytotoxicity [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

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Isolation of cfDNA from spent culture media and its association with implantation rate and maternal immunomodulation

et al. 2022

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Objectives This investigation aims to evaluate the association between the concentration of cell-free DNA (cfDNA) in the spent culture medium (SCM) with implantation rate and the maternal immune system in the invitro fertilization (IVF). In this study, 30 embryos were cultured and scored according to Gardner's criteria. SCM was gathered on day five from every embryo to analyze the quantity of cfDNA. The real-time PCR technique evaluated the expression level of transcription factors, including Foxp3, RORγt, GATA3, and T-bet. The percentage of Th1, Th2, Th17, Treg, NK cells, and NK cells cytotoxicity was evaluated by flow cytometry. Results The concentration of cfDNA in the β-HCG (-), β-HCG ( +), and ongoing pregnancy groups were 20.70 ± 9.224 ng/µL, 27.97 ± 7.990 ng/µL, and 28.91 ± 8.566 ng/µL, respectively. The ratio of Th1/Th2 and Th17/Treg reduced significantly in pregnant women, as well as the level of NK cells and NK cytotoxicity cells fell dramatically in the ongoing pregnancy group. The expression level of RORγt and T-bet declined while the expression level of Foxp3 and GATA3 increased considerably in pregnant mothers. Our investigation revealed that the concentration level of cfDNA in SCM could not be associated with implantation rate, prediction of ongoing pregnancy, and maternal immune system.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Isolation of cfDNA from spent culture media and its association with implantation rate and maternal immunomodulation

et al. 2022

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“…The soluble form of Flt1 is created by alternatively spliced mRNA that binds and inhibits VEGF and placental growth factor (PlGF) signaling [ 69 ]. The excess level of sFlt-1 has been demonstrated to induce endothelial dysfunction, particularly in pre-eclampsia [ 70 , 71 ]. In previous studies, sFlt-1 was identified as a biological marker of endothelial dysfunction associated with bacterial sepsis and severity [ 72 ], and it was increased in COVID-19 patients [ 69 , 73 ].…”

Section: Other Soluble Mediatorsmentioning

confidence: 99%

Immune system-related soluble mediators and COVID-19: basic mechanisms and clinical perspectives

et al. 2022

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During SARS-CoV-2 infection, an effective immune response provides the first line of defense; however, excessive inflammatory innate immunity and impaired adaptive immunity may harm tissues. Soluble immune mediators are involved in the dynamic interaction of ligands with membrane-bound receptors to maintain and restore health after pathological events. In some cases, the dysregulation of their expression can lead to disease pathology. In this literature review, we described current knowledge of the basic features of soluble immune mediators and their dysregulation during SARS-CoV-2 infections and highlighted their contribution to disease severity and mortality.

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“…TGF‐β is necessary for the maturation and differentiation of Treg cells, and any dysregulation in this miRNA may alter the activity of Treg cells 96 . Moreover, miR‐326 affects the differentiation of Th17 lymphocytes by preventing Ets‐1 activity 97 . It has been proven that Ets‐1 has an important role in the modulation of Treg and Th17 cell responses 98 .…”

Section: Micrornas and Their Function In Pementioning

confidence: 99%

“…96 Moreover, miR-326 affects the differentiation of Th17 lymphocytes by preventing Ets-1 activity. 97 It has been proven that Ets-1 has an important role in the modulation of Treg and Th17 cell responses. 98 Eghbal-fard et al 46 reported that the expression of miR-106b and miR-326 is upregulated in PE women, and the dysregulation of these two miRNAs can affect the Th17/Treg balance and make a shift toward Th17 response in PE patients.…”

Section: Dysregulation Of Mirnas Related To T Lymphocytes In Pementioning

confidence: 99%

T lymphocytes and preeclampsia: The potential role of T‐cell subsets and related MicroRNAs in the pathogenesis of preeclampsia

Zolfaghari

ArefNezhad

Parhizkar

et al. 2021

American J Rep Immunol

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Innate and adaptive immune systems have a crucial role in initiating and progressing some pregnancy disorders such as preeclampsia (PE), which is one of the pregnancy‐specific disorders that could result in neonatal and maternal morbidity and mortality. The dysregulation of the spiral artery and inadequate trophoblast invasion lead to PE symptoms through producing various inflammatory cytokines and anti‐angiogenic factors from the placenta. T lymphocytes play a special role in the epithelium and stroma of the human endometrium. CD4+ T helper (Th) cells, Th1/Th2, and Th17/T regulatory (Treg) balance mainly contribute to the establishment of a pregnancy‐favorable environment. This review examined the dysregulation of some cytokines produced from T cells, the dysregulation of the transcription factors of Th cells, the expression of chemokine receptors on T cells, as well as the effects of some factors including vitamin D on the activity of T cells, and finally, the dysregulation of various miRNAs related to T cells, which could cause PE.

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A new approach to the preeclampsia puzzle; MicroRNA-326 in CD4+ lymphocytes might be as a potential suspect

Cited by 17 publications

References 44 publications

Isolation of cfDNA from spent culture media and its association with implantation rate and maternal immunomodulation

Isolation of cfDNA from spent culture media and its association with implantation rate and maternal immunomodulation

Immune system-related soluble mediators and COVID-19: basic mechanisms and clinical perspectives

T lymphocytes and preeclampsia: The potential role of T‐cell subsets and related MicroRNAs in the pathogenesis of preeclampsia

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