A New Arenavirus in a Cluster of Fatal Transplant-Associated Diseases

Palacios, Gustavo; Druce, Julian; Du, Lei; Tran, Thomas; Birch, Chris; Briese, Thomas; Conlan, Sean; Quan, Phenix‐Lan; Hui, Jeffrey; Marshall, John; Simons, Jan Fredrik; Egholm, Michael; Paddock, Christopher D.; Shieh, Wun‐Ju; Goldsmith, Cynthia S.; Zaki, Sherif R.; Catton, Mike; Lipkin, W. Ian

doi:10.1056/nejmoa073785

Cited by 644 publications

(503 citation statements)

References 12 publications

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“…There was, however, a serious systemic disease resembling that caused by another arenavirus, LF virus. Four transplant donors who were apparently infected with LCMV at the time of organ harvest have transmitted an often fatal LCMV infection to the organ recipients (Fischer et al, 2006;Anonymous, 2008;Palacios et al, 2008;Macneil et al, 2012). The 14 solid-organ recipients (but not cornea recipients) were all infected with LCMV and suffered a difficult Lassa-like clinical course, resulting in 11 deaths.…”

Section: Lcmv Infection In Pregnancymentioning

confidence: 99%

“…The highly sensitive assay targets the GPC and N genes. Lastly, as the most recent transplant- associated cases illustrate, newer unbiased diagnostic methods are becoming available that will not only facilitate improved detection of known viruses but also allow for the identification of wholly new viral pathogens (Palacios et al, 2008;Briese et al, 2009). …”

Section: Diagnosismentioning

confidence: 99%

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Diseases of the central nervous system caused by lymphocytic choriomeningitis virus and other arenaviruses

Wilson¹,

Peters²

2014

Neurovirology

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Section: Lcmv Infection In Pregnancymentioning

confidence: 99%

Section: Diagnosismentioning

confidence: 99%

Diseases of the central nervous system caused by lymphocytic choriomeningitis virus and other arenaviruses

Wilson¹,

Peters²

2014

Neurovirology

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“…Likewise, several New World arenaviruses, chiefly Junin virus, cause viral HF disease (3). Moreover, evidence indicates that the worldwide distributed LCMV is a neglected human pathogen of clinical significance (4) and poses a special threat to immunocompromised individuals (5,6).…”

mentioning

confidence: 99%

Generation of recombinant lymphocytic choriomeningitis viruses with trisegmented genomes stably expressing two additional genes of interest

Emonet

Garidou

McGavern

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

124

216

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Several arenaviruses cause hemorrhagic fever disease in humans for which no licensed vaccines are available and current therapeutic intervention is limited to the off-label use of the wide-spectrum antiviral ribavirin. However, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) has proven to be a Rosetta stone for the investigation of virus-host interactions. Arenaviruses have a bisegmented negative-strand RNA genome. The S segment encodes for the virus nucleoprotein and glycoprotein, whereas the L segment encodes for the virus polymerase (L) and Z protein. The ability to generate recombinant LCMV (rLCMV) expressing additional foreign genes of interest would open novel avenues for the study of virushost interactions and the development of novel vaccine strategies and high-throughput screens to identify antiarenaviral molecules. To this end, we have developed a trisegmented (1L ؉ 2S) rLCMV-based approach (r3LCMV). Each of the two S segments in r3LCMV was altered to replace one of the viral genes by a gene of interest. All r3LCMVs examined expressing different reported genes were stable both genetically and phenotypically and exhibited wild-type growth properties in cultured cells. Reporter gene expression in r3LCMV-infected cells provided an accurate surrogate of levels of virus multiplication. Notably, some r3LCMVs displayed highly attenuated virulence in mice but induced protective immunity against a subsequent lethal challenge with wild-type LCMV, supporting the potential development of r3LCMV-based vaccines.antiviral screen ͉ reverse genetic ͉ viral attenuation A renaviruses merit significant interest both as tractable experimental model systems to study acute and persistent viral infections and as clinically important human pathogens (1-3). Thus, the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV) has proven to be a superb workhorse in the field of virology and immunology (1). However, several arenaviruses cause hemorrhagic fever (HF) disease in humans, associated with high morbidity and mortality (2, 3). The Old World arenavirus Lassa virus (LASV) poses the highest public health concern among HF arenaviruses. LASV is estimated to infect several hundred thousand individuals yearly in its endemic region of West Africa, resulting in a high number of Lassa fever disease cases (2). Likewise, several New World arenaviruses, chiefly Junin virus, cause viral HF disease (3). Moreover, evidence indicates that the worldwide distributed LCMV is a neglected human pathogen of clinical significance (4) and poses a special threat to immunocompromised individuals (5, 6).Public health concerns posed by human pathogenic arenaviruses are aggravated by the lack of licensed vaccines and current therapy being limited to the use of the nucleoside analog ribavirin (Rib) that can cause significant side effects and requires an early and i.v. administration for optimal efficacy (3). Therefore, it is important to develop novel effective antiarenaviral drugs and vaccines, tasks that would be facilitated ...

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“…To identify primers and probes, conserved regions specific to LCMV were screened based on an extensive alignment of all LCMV and Old World arenavirus's sequences available in Genbank in August 2010, including all recently discovered arenaviruses (Briese et al, 2009;Gunther et al, 2009;Palacios et al, 2008). This alignment allowed to pinpoint a conserved region within the viral nucleoprotein (NP) gene.…”

Section: Introductionmentioning

confidence: 99%

Analytical validation of a lymphocytic choriomeningitis virus real-time RT-PCR assay

Cordey

Sahli

Moraz

et al. 2011

Journal of Virological Methods

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A New Arenavirus in a Cluster of Fatal Transplant-Associated Diseases

Cited by 644 publications

References 12 publications

Diseases of the central nervous system caused by lymphocytic choriomeningitis virus and other arenaviruses

Diseases of the central nervous system caused by lymphocytic choriomeningitis virus and other arenaviruses

Generation of recombinant lymphocytic choriomeningitis viruses with trisegmented genomes stably expressing two additional genes of interest

Analytical validation of a lymphocytic choriomeningitis virus real-time RT-PCR assay

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