A New Aspect for In vitro Propagation of Jerusalem Artichoke and Molecular Assessment Using RAPD, ISSR and SCoT Marker Techniques

Abdalla, Neama; Ragab, M. T.; El-Miniawy, Salah; Arafa, Nermeen; Taha, Hussein

doi:10.21608/ejbo.2020.40297.1541

Cited by 5 publications

(12 citation statements)

References 40 publications

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“…Endophytic microbes that are present within the explants are considered a major constrain to the establishment and growth of tissue-cultured plants, as they are more difficult to remove by normal surface sterilization. However, the internal bacterial contamination could be eliminated by supplying the culture media with different substances (Table 2), for example, antibiotics [44,45], copper sulfate [44,46], or fungicides [47]. After identification of the contaminants, a low-phytotoxicity antibiotic should be selected [47].…”

Section: Sargassum Fusiformementioning

confidence: 99%

“…Antibiotic, cefotaxime at 62.5 mg L −1 was supplemented to 1 2 Murashige and Skoog (MS) medium for establishment Jerusalem artichoke (Helianthus tuberosus L.) It recorded 0% contamination 100% survival of stem nodes cultures. [45] Copper sulfate (CuSO 4 5H 2 O) at 60 mg L −1 was added to MS medium +3 mg L −1 BA + 1 mg L −1 KIN for shoot multiplication Banana (Musa sp. )…”

Section: Guadua Angustifolia Kunthmentioning

confidence: 99%

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An Academic and Technical Overview on Plant Micropropagation Challenges

et al. 2022

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The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

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Section: Sargassum Fusiformementioning

confidence: 99%

Section: Guadua Angustifolia Kunthmentioning

confidence: 99%

An Academic and Technical Overview on Plant Micropropagation Challenges

et al. 2022

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“…Selenium NPs have a wide range of applications in medical and agricultural elds (Shoeibi et al 2017). It was reported that selenium nanoparticles stimulate callus induction and improve morphogenesis of tobacco and increased shoot length and shoot number of in vitro growing artichoke (Abdalla et al 2021;Domokos-Szabolcsy et al 2012). However more studies are required to clarify the possible effect of selenium and chitosan NPs on in vitro plant tissues culture.…”

Section: )mentioning

confidence: 99%

Enhancing In Vitro Multiplication of some Olive Cultivars Using Silver, Selenium And Chitosan Nanoparticles

Darwesh

Hassan

Abdallatif

2021

Preprint

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Olive trees are commercially propagated by cuttings or grafting on clonal or seedling rootstocks; recently, olive micropropagation has emerged as a powerful technique for mass production of true to type and pathogen free plants. The current study was carried out to evaluate the effect of silver, chitosan and selenium nanoparticles as microbial decontamination agents, as well as evaluate its possible effects on in vitro shoot growth and multiplication of three olive cultivars namely Koroneiki, Picual and Manzanillo. Validation and characterization of the biosynthesized nanoparticles was carried out by Transmission Electron Microscopy. The produced nanoparticles were added to the olive culture medium, and the growth parameters were determined. The tested nanoparticles showed varied degree of antimicrobial activity, silver nanoparticles were highly effective to inhibit in vitro microbial contamination. The effect of genotypes on shoot growth and multiplication was significant; Koroneiki and Picual cultivars showed better growth measurements compared with Manzanillo. The addition of nanoparticles to the culture medium had a significant outcome on growth and multiplication rate of in vitro growing olive shoots. Silver nanoparticles produced higher values of number of shoots, shoots length, leaf number and multiplication rate compared with the other treatments. In conclusion; the current results showed that nanoparticles were efficient as in vitro disinfectant agent and the nanoparticles addition to culture medium especially silver NPs had a positive effect on growth and multiplication rate of in vitro growing olive shoots.

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“…During the past few decades, in vitro culture techniques have been explored for Jerusalem artichokes for rapid clonal propagation [13,14], microtuber induction [15], germplasm cryopreservation [16], inulin production [15], seed dormancy breaking [6], and genetic transformation [17]. Generally, callus induction is an important step in the in vitro regeneration protocols of many plants [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Plant Regeneration via Organogenesis in Jerusalem Artichokes and Comparative Analysis of Endogenous Hormones and Antioxidant Enzymes in Typical and Atypical Shoots

Zhang,

Yin

et al. 2023

Plants

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The Jerusalem artichoke (Helianthus tuberosus) is a tuberous plant with considerable nutrient and bioactive compounds. The optimization of the in vitro clonal propagation protocol is critical for large-scale reproduction and biotechnological applications of Jerusalem artichoke production. In this work, in vitro plant regeneration from the stem nodes of the Jerusalem artichoke via direct organogenesis is presented. In the shoot induction stage, the stem segments produced more shoots with vigorous growth on MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA). The concentrations of 6-BA and gibberellic acid (GA3) were both optimized at 0.5 mg/L for shoot multiplication, and the combination of 0.05 mg/L indole-3-butyric acid (IBA) and 0.05 mg/L 1-naphthylacetic acid (NAA) was the most responsive for root induction, yielding the largest number of roots. The regenerated plantlets were successfully hardened at a 96% survival rate and vigorously grew in the field. The genetic stability of the regenerated plants was confirmed by flow cytometry and simple sequence repeat (SSR) analysis. However, 17.3% of shoots on the optimum shoot induction medium had withered leaves and excessive callus (atypical shoots), which greatly reduced the induction efficiency. Enzyme activity in the typical and atypical shoots was compared. The atypical shoots had significantly higher levels of endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA), as well as increased activity of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), whereas the content of 6-BA, zeatin (ZT), and GA3 was significantly reduced. The activity of the three enzymes was positively correlated with the content of IAA and ABA, while being negatively correlated with that of 6-BA, ZT, and GA3. The results suggest that the poor growth of the atypical shoots might be closely related to the significant accumulation of endogenous IAA and ABA, thus significantly increasing antioxidant enzyme activity.

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A New Aspect for In vitro Propagation of Jerusalem Artichoke and Molecular Assessment Using RAPD, ISSR and SCoT Marker Techniques

Cited by 5 publications

References 40 publications

An Academic and Technical Overview on Plant Micropropagation Challenges

An Academic and Technical Overview on Plant Micropropagation Challenges

Enhancing In Vitro Multiplication of some Olive Cultivars Using Silver, Selenium And Chitosan Nanoparticles

Plant Regeneration via Organogenesis in Jerusalem Artichokes and Comparative Analysis of Endogenous Hormones and Antioxidant Enzymes in Typical and Atypical Shoots

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