A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies

Wang, Junrong; Bai, Juan; Zhang, Lujie; Xia, Tingting; Yang, Xing; Zhang, Keshan; Gao, Yanni; Jiang, Ping

doi:10.1016/j.vetmic.2023.109744

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…A definite diagnosis of ASF by clinical signs is not possible; therefore, laboratory testing techniques are often used for diagnostic evaluation. The most common laboratory testing techniques are pathogen assays and serologic assays [ 13 ]. Pathogen assays detect viruses directly at the antigenic or genetic level and include polymerase chain reaction (PCR), erythrocyte adsorption assay, macrophage virus isolation, direct fluorescent antibody assay, and antigen-based enzyme-linked immunosorbent assay (ELISA) [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Stability of African swine fever virus genome under different environmental conditions

Zheng,

Xi,

et al. 2023

Vet World

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Background and Aim: African swine fever (ASF), a globally transmitted viral disease caused by ASF virus (ASFV), can severely damage the global trade economy. Laboratory diagnostic methods, including pathogen and serological detection techniques, are currently used to monitor and control ASF. Because the large double-stranded DNA genome of the mature virus particle is wrapped in a membrane, the stability of ASFV and its genome is maintained in most natural environments. This study aimed to investigate the stability of ASFV under different environmental conditions from both genomic and antibody perspectives, and to provide a theoretical basis for the prevention and elimination of ASFV. Materials and Methods: In this study, we used quantitative real-time polymerase chain reaction for pathogen assays and enzyme-linked immunosorbent assay for serological assays to examine the stability of the ASFV genome and antibody, respectively, under different environmental conditions. Results: The stability of the ASFV genome and antibody under high-temperature conditions depended on the treatment time. In the pH test, the ASFV genome and antibody remained stable in both acidic and alkaline environments. Disinfection tests revealed that the ASFV genome and antibody were susceptible to standard disinfection methods. Conclusion: Collectively, the results demonstrated that the ASFV genome is highly stable in favorable environments but are also susceptible to standard disinfection methods. This study focuses on the stability of the ASFV genome under different conditions and provides various standard disinfection methods for the prevention and control of ASF. Keywords: African swine fever, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, standard disinfection methods, virus stability.

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Section: Introductionmentioning

confidence: 99%

Stability of African swine fever virus genome under different environmental conditions

Zheng,

Xi,

et al. 2023

Vet World

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“…ASFV is a large, enveloped virus with icosahedral morphology and belongs to the Asfarviridae family, isolates of which have linear dsDNA genomes of 170–194 kbp, encoding more than 150 polypeptides [ 5 ]. Monoclonal antibodies against various ASFV proteins, including the major capsid protein p72, the viral internal envelope protein p30 and p17, the outer envelope protein CD2v, the type II transmembrane protein p54, the nonstructural protein pK205R, and the uncharacterized protein pCP312R and pC129R, have been generated for ASFV infection research and development of ASFV diagnosis [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ]. pI215L, a nonstructural protein of ASFV, is found within the virion and expressed at a high level in infected porcine alveolar macrophages (PAMs) from 4 h post-infection (hpi) [ 14 , 15 ], indicating a possible role of pI215L protein in the early steps of infection.…”

Section: Introductionmentioning

confidence: 99%

Novel Epitope Mapping of African Swine Fever Virus pI215L Protein Using Monoclonal Antibodies

Gao,

Jiang,

Yang

et al. 2023

Viruses

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The African swine fever virus (ASFV) is one of the most important pathogens that causes huge damage to worldwide swine production. The pI215L protein is found within the virion and expressed at a high level in infected porcine alveolar macrophages (PAMs), indicating a possible role of pI215L protein in ASFV detection and surveillance. In the present study, female BALB/c mice (5–6-week-old) were immunized with rpI215L protein, and six hybridomas, 1C1, 2F6, 2F10, 3C8, 5E1 and 5B3, steadily secreted anti-pI215L monoclonal antibodies (mAbs). Among them, 1C4, 5E1, and 5B3 had the IgG1 isotype with a Lambda light chain, 2F10 and 3C8 had the IgG1 isotype with a Kappa light chain, and 2F6 had the IgG2a isotype with a Kappa light chain. Western blot showed a good reactivity of the six mAbs against ASFV. Eight truncated polypeptides were produced for epitope mapping. Two novel B cell epitopes, 67LTFTSEMWHPNIYS80 and 167IEYFKNAASN176, were identified by the mAbs. Further analysis revealed that 2F6 mAb could be widely used in ASFV surveillance and 5B3 mAb might serve as a tool in the distinguishment of different ASFV genotypes. This study provides tools of monoclonal antibodies for further study of I215L function and contributes to the development of serological diagnosis and vaccine research.

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Development and characterization of a novel specific monoclonal antibody against mink enteritis virus and its antigen epitope analysis

Sun,

Li,

Wang

et al. 2024

Microbial Pathogenesis

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A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies

Cited by 6 publications

References 21 publications

Stability of African swine fever virus genome under different environmental conditions

Stability of African swine fever virus genome under different environmental conditions

Novel Epitope Mapping of African Swine Fever Virus pI215L Protein Using Monoclonal Antibodies

Development and characterization of a novel specific monoclonal antibody against mink enteritis virus and its antigen epitope analysis

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