A new coordination polymer for selectively detect TNP and its inhibition activity on P.gingivalis growth by reducing ragA and ragB gene expression

Wang, Xu; Li, Daiqing; Zhao, Jun; Wei, Chao

doi:10.1007/s10965-019-1958-3

Cited by 5 publications

(9 citation statements)

References 22 publications

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“…Afterwards, the suspension was inoculated to 96-well plates (Nest) and mixed with a series of concentrations of D-AAs, consequently incubated for 72 hours at 37 °C under anaerobic conditions. Simultaneously, the OD 600 value was measured every 4 hours with a spectrophotometer (Bole Life) 34 . The minimum concentration of sample showing no turbidity at 72 hours was recorded as the minimal inhibitory concentrations (MICs).…”

Section: Methodsmentioning

confidence: 99%

Synergistic effects of D-arginine, D-methionine and D-histidine on the inhibition and eradication of Porphyromonas gingivalis biofilm

Zhang

Cai

et al. 2020

Preprint

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Porphyromonas gingivalis (P. gingivalis) biofilm is involved in peri-implantitis and periodontitis. Whether a mixture of D-AAs has synergistic effects on P. gingivalis biofilm remains unknown. The aim of this study was to investigate the effects of multiple D-AAs on P. gingivalis biofilm. D-arginine (R), D-methionine (M) and D-histidine (H) were used. The bacterial growth activity and minimum inhibitory concentrations (MICs) were determined. The effects of D-AAs mixture on biofilm biomass, extracellular polymeric substances (EPS), biofilm morphology, cytotoxicity, biofilm structure and bacterial membrane integrity were determined. D-AAs mixture delayed the proliferation of P. gingivalis below MICs. Mixtures of D-AAs displayed definite effects on the inhibition and disassembly of biofilm than single D-AAs. The EPS content increased with D-AAs concentrations. D-AAs mixture damaged the integrity of biofilm, while RMH showed the best effects. 8 mM RH and 4 mM RMH had no cytotoxicity. 4 mM RMH decreased biofilm thickness and altered bacteria membranes structure. Hence, the combined mixture of 4 mM RMH presents the best synergistic effects on the inhibition and eradication of P. gingivalis biofilm. Our study provides new insights of application of D-AAs for the treatment of peri-implantitis and periodontitis.

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Section: Methodsmentioning

confidence: 99%

Synergistic effects of D-arginine, D-methionine and D-histidine on the inhibition and eradication of Porphyromonas gingivalis biofilm

Zhang

Cai

et al. 2020

Preprint

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“…Finally, 15 articles were chosen based on inclusion and exclusion criteria. All the scientific publications examined the in vitro antibacterial activity of zinc-ion-containing substances on at least one bacterium associated with periodontal diseases [4,[8][9][10][11][12][13][14][15][16][17][18][19][20][21], while four publications investigated the toxicity of Zn compounds on gingival fibroblasts [4,8,10,12]. The activity of zinc against bacteria was studied during 24 and 48 h periods, with the longest test duration being three days [20].…”

Section: General Characteristicmentioning

confidence: 99%

“…Antibacterial activity was tested using different substances in various concentrations. ZnO nanoparticles were the primary test substance in four publications [8,10,16,18], while 11 publications also examined an additional zinc-containing compound [4,9,[11][12][13][14][15]17,[19][20][21]. In one publication, free ZnO was studied in combination with an additional substance, polycaprolactone [4].…”

Section: A Actinomycete Mcomitans (150 µL)mentioning

confidence: 99%

“…They also synthesized zinc peroxide together with bioactive nanomolecules of sugar and assessed the release of oxygen and antibacterial activity [14]. Additionally, researchers have analyzed a material supporting bivalent zinc (Zn(NO 3 ) 2 •6H 2 O) [11,21], synthesized a polymer supporting bivalent zinc [Zn(TBTA) (L)1.5]n [17], modified the surfaces of titanium implants with zinc [20], and synthesized dextran-coated zinc hydroxyapatite. Dextran has natural resistance to salivary microorganisms, and dextranase acts on plaque bacteria.…”

Section: A Actinomycete Mcomitans (150 µL)mentioning

confidence: 99%

“…Scientists are still studying the effects of zinc on oral bacteria and inflammatory periodontal tissues. The results should help answer whether the topical application of zinc (as part of a medication system) can accelerate or enhance the healing of periodontal tissues as this substance can be released directly to the target location at constant concentrations [4,[8][9][10][11][12][13][14][15][16][17][18][19][20][21]. Topical administration of zinc medications into periodontal tissues could provide prolonged action and effectiveness [9].…”

Section: Introductionmentioning

confidence: 99%

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Antimicrobial Activity of Zinc against Periodontal Pathogens: A Systematic Review of In Vitro Studies

Griauzdyte,

Jagelaviciene

2023

Medicina

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Background and Objectives: More than a billion people worldwide suffer from chronic periodontitis. The primary etiological factor of periodontal diseases is dental plaque and the bacteria it contains, particularly Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Prevotella intermedia, and Aggregatibacter actinomycetemcomitans. Zinc, owing to its antibacterial properties, can be employed in periodontology. The objective of this review was to analyze scientific literature that examines the effects of zinc on periopathogens. Materials and methods: A systematic review protocol of scientific literature was designed following PRISMA recommendations. Data search was conducted in PubMed, Web of Science, and ScienceDirect databases. Full-text articles in English that examine the effects of zinc on periopathogens and were published between 2011 and 2021 were included. Results: Fifteen articles were included in the analysis based on inclusion criteria. ZnO exhibited antibacterial activity against P. gingivalis and P. intermedia (p < 0.001). The minimum inhibitory concentration against P. gingivalis was 10 μg/mL. ZnO demonstrated a significant antibacterial effect, as evidenced by inhibition zones of 15.10 mm for S. oralis, 13.36 mm for P. gingivalis, 12.98 mm for S. sanguis, and 14.01 mm for P. intermedia. Zn (II)-based polymers inhibited the ragA and ragB genes of P. gingivalis. Titanium dental implants coated with ZnO effectively disrupted the cell walls of P. gingivalis and A. actinomycetemcomitans. ZnO inhibited the growth of P. gingivalis within 2 h and the growth of F. nucleatum and P. intermedia within 3 h. ZnO exhibited nontoxic effects, and concentrations up to 0.8 mg/L increased cell survival rates by up to 90%. Conclusions: The analysis of the literature confirms the antibacterial action of zinc against periodontal pathogenic bacteria. At low concentrations, these substances do not exhibit cytotoxic effects on fibroblasts.

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RETRACTED ARTICLE: A fluorescent Zn(II)-containing coordination polymer for antibiotic sensing and treatment activity on periodontal tissue inflammation after orthodontics by inhibiting the growth of Porphyromonas gingivalis

2020

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A new coordination polymer for selectively detect TNP and its inhibition activity on P.gingivalis growth by reducing ragA and ragB gene expression

Cited by 5 publications

References 22 publications

Synergistic effects of D-arginine, D-methionine and D-histidine on the inhibition and eradication of Porphyromonas gingivalis biofilm

Synergistic effects of D-arginine, D-methionine and D-histidine on the inhibition and eradication of Porphyromonas gingivalis biofilm

Antimicrobial Activity of Zinc against Periodontal Pathogens: A Systematic Review of In Vitro Studies

RETRACTED ARTICLE: A fluorescent Zn(II)-containing coordination polymer for antibiotic sensing and treatment activity on periodontal tissue inflammation after orthodontics by inhibiting the growth of Porphyromonas gingivalis

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