A new coronavirus associated with human respiratory disease in China

Abstract

The Pandemic situation caused due to SARS-CoV-2 causing Coronavirus Disease (CoVID-19) around globe. Recent, COVID-19 main protease complex (M pro), highly modulating enzyme in SARS-CoV-2 was reported for viral replication and transcription. This multifunctionality of M pro attracts for identi cation of potential drug target. Considering impact, In silico analysis was performed for Palmatine alkaloid against M pro. Naturally, present in Tinospora cordifolia, found effective against Cancer, HIV, viral infections, diabetics. In methods, physico-chemical analysis by ProtParam tool and Structure of M pro was predicted by SWISS-MODEL Workspace homology modeling server. Superimposition Structure and signi cant equal QMQE, QSQE values were found for eight highly similar templates. Structural assessment validation by Ramachandran plot (97.67% favoured), Local Quality estimate ratio (>0.6) and higher QMEAN score (y-axis). Further, docking was performed with validated M pro model by SwissDock server. Interaction with-8.281919 ΔG indicates reliable Interaction. Also, comparative docking reveals, most favoured Palmatine interaction. Thus, an attempt was made to nd potent inhibitor for SARS-CoV-2, as there is no promising and speci c anti-viral drug or vaccine available for prevention and treatment of infections. However, In Vitro studies are required. Toxicity studies reported against Palmatine for acute effect (135 mg/kg body weight) on mouse model LD 50.

The deadly coronaviruses: The 2003 SARS pandemic and the 2020 novel coronavirus epidemic in China

“…The genomic structure is shown in Fig. 7 and shows greater than 99.9% consistency [19,[91][92][93][94][95].…”

“…The phylogenetic tree based on whole genomes showed that SARS-CoV-2 is most closely related to bat SARS-like coronavirus bat-SL-CoVZC21 (NCBI accession number MG772934) and bat-SL-CoVZC45 (NCBI accession number MG772933), which share~89% sequence homology [91][92][93]. Their genomic organization is typical of a lineage B beta coronavirus.…”

Identification of the hyper-variable genomic hotspot for the novel coronavirus SARS-CoV-2

“…The sliding window was set to 500 for ORF1ab and S, and to 50 for proteins E, M, and N considering their short length. SARS-CoV-2 were highly similar to RaTG13 isolated from bats, showing 96% identity based on the whole-nucleotide sequences and 83% based on the protein 3 sequences, suggesting a bat zoonotic origin of SARS-CoV-2. ORF1a, and the head of S seemed to have diverged from other beta coronaviruses.…”

“…By February 14, 2020, a total of 66,576 2 confirmed cases of COVID-19, people infected with SARS-CoV-2, were reported in China, leading to 1,524 deaths, per the Chinese CDC (http://2019ncov.chinacdc.cn/2019-nCoV/). Several full genomic sequences of this virus have been released for the study of its evolutionary origin and molecular characteristics [2][3][4]. Here, we analyzed the potential mutations that may have evolved after the virus became epidemic among humans and also the mutations resulting in the human adaptation.…”

Simulating and forecasting the cumulative confirmed cases of SARS-CoV-2 in China by Boltzmann function-based regression analyses

“…As of Feb 16, 2020 (24:00, GMT+8), there have been 70548 confirmed patients and more than 1700 deaths from SARS-CoV-2 infection in China, and 58182 confirmed patients and 1696 deaths in the most affected province, Hubei Province. Much research progress has been made in dissecting the evolution and origin of SARS-CoV-2 and characterizing its clinical features [3][4][5][6][7] .…”