A new device for the prompt diagnosis of urinary tract infections

Arienzo, Alyexandra; Losito, Francesca; Bottini, Giorgia; Priolisi, Francesca Romana; Mari, A.; Visca, Paolo; Antonini, Giovanni

doi:10.1515/cclm-2014-0294

Cited by 4 publications

(6 citation statements)

References 14 publications

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“…The MBS method allows rapid and accurate bacterial quantification through an automated colorimetric culture-based test; urine samples are inoculated into disposable ready-to-use reaction vials, which color will change thanks to redox indicators following bacterial growth after incubation (see Figure 1). Results of preliminary in vitro validation studies [62,63] showed that the results obtained with this method are comparable to the reference culture-based methods.…”

Section: The Importance Of Point-of-care Tests In Uti Diagnosismentioning

confidence: 68%

Management of Urinary Tract Infections: Problems and Possible Solutions

Murgia¹,

Stalio²,

Arienzo³

et al. 2018

Urinary Tract Infection - The Result of the Strength of the Pathogen, or the Weakness of the Host

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In clinically suspected urinary tract infections (UTIs), empirical antibiotic treatment is usually started long before the laboratory results of urine culture and antibiogram are available. Although molecular diagnostic approaches are being applied to the diagnosis of many infections, UTIs are generally diagnosed by traditional culture methods. Patient care could greatly benefit from the development of a rapid, accurate, inexpensive test that could be done at patient's bedside, allowing the practitioner to plan targeted, more effective therapy. Such a test would potentially reduce incorrect or unnecessary use of antibacterial drugs and reduce the emergence of bacterial resistance. In response to this pressing and unmet clinical need, several methods have been developed in the last few years. Among these, the new point-of-care test (POCT) for detecting UTIs named Micro Biological Survey (MBS) UTI CHECK holds promise, as it allows semi-quantitative determination of bacterial load in urine leading to a fast detection of UTIs and to evaluation of bacterial antibiotic susceptibility. This new technology operates through a colorimetric survey performed in low-cost, ready-to-use, disposable vials, in which 1 ml of urine is inoculated without any preliminary treatment and requiring neither specialized personnel nor a specialized equipment.

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Section: The Importance Of Point-of-care Tests In Uti Diagnosismentioning

confidence: 68%

Management of Urinary Tract Infections: Problems and Possible Solutions

Murgia¹,

Stalio²,

Arienzo³

et al. 2018

Urinary Tract Infection - The Result of the Strength of the Pathogen, or the Weakness of the Host

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“…[ 23 ] showed the existence of a direct impact of organic enrichment on the benthic invertebrate communities of a river through a study confronting sites which were situated upstream and downstream of several sewage treatment works [ 32 ]. Besides this, [ 19 ] showed the existence of a significant correlation between E . coli and EBI, this allowing to emphasize the importance of using both information to obtain a wider definition of the environmental status of a river [ 22 ].…”

Section: Discussionmentioning

confidence: 95%

Integrating running water monitoring tools with the Micro Biological Survey (MBS) method to improve water quality assessment

et al. 2017

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Running water habitats are among the most altered aquatic systems by human activities driving an increase in the organic components and the associated bacterial load as well. To contribute in improving the monitoring activities in running waters, here we tested the validity of the new Micro Biological Survey (MBS) method to specifically assess the bacterial load in running waters focusing on Total Viable Counts (at 22°C and 37°C) and Escherichia coli (at 44°C) in order to propose a new prognostic tool for watercourses. MBS method is an alternative colorimetric method for counting bacterial load in water and food samples that is easy to use and leads to a reliable and simple interpretation of results, being also faster and less expensive than traditional methods. Then, we compared MBS with the traditionally used reference method for the bacterial load, and with the most used biotic index for Italian watercourses based on the benthic invertebrates: the Extended Biotic Index (EBI). The last comparison was performed to validate the use of MBS in biomonitoring activities since the benthic invertebrate multi-species assemblage (and then EBI) alter own structure mainly depending on the organic component variation. During the first part of the study, the assessment of both linearity (regressions among bacterial concentrations) and accuracy (significant correlation between a measured value and a value used as reference) confirmed the validity of the MBS method. Second, the linear regressions between the three investigated microbial parameters vs. both physical-chemical descriptors and EBI, revealed the usefulness of MBS as a valid tool for routine microbiological analyses involved in rapid and easy field monitoring activities. This represents the first attempt to evaluate the river microbial status by exploiting the innovative MBS on running waters to propose it as new valuable monitoring tool in the biomonitoring field.

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“…The MBS AST-POCT analysis is performed in ready to use disposable vials containing the lyophilized growth medium used for the detection of UTI [ 31 ] alone or supplemented with selected antibiotics, namely amoxicillin, ciprofloxacin, trimethoprim-sulfamethoxazole and levofloxacin, chosen as first-line treatment options according to hospital guidelines. Antibiotics are added at clinical breakpoint concentrations according to European Committee on Antimicrobial Susceptibility Testing [ 32 ]: 8 μg/ml for amoxicillin-clavulanic acid, 1 μg/ml for ciprofloxacin, 4 μg/ml for trimethoprim-sulfamethoxazole, 1 μg/ml for levofloxacin.…”

Section: Methodsmentioning

confidence: 99%

“…This POCT demonstrated to be accurate and time saving: performed in ready to use, disposable reaction vials and based on the time taken for the reaction vials to change color. It allows a quantitative assessment in of viable bacteria concentration in urine samples in less than 5 hours, not needing other instrumentation than a dedicated thermostatic reader which automatically provides results, opening for automation and processing of multiple samples [ 30 , 31 ]. The same methodology was implemented in a new POCT for antibiotic susceptibility testing, developed by MBS Diagnostics ltd (London, UK), which is the focus of this work.…”

Section: Introductionmentioning

confidence: 99%

A new point-of-care test for the rapid antimicrobial susceptibility assessment of uropathogens

et al. 2023

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Bacterial resistance to antimicrobials is considered a major issue worldwide. This condition may account for treatment failure of urinary tract infections, which are among the most common infections both in community and healthcare settings. Therapy against uropathogens is generally administered empirically, possibly leading to unsuccessful therapy, recurrence and development of antibiotic resistance. The reduction in analytical time to obtain antimicrobial susceptibility test (AST) results could play a key role in reducing the cost of healthcare, providing information about antibiotic efficacy and thus preventing from either exploiting new and expensive antibiotics unnecessarily or using obsolete and ineffective ones. A more rational choice among treatment options would hence lead to more effective treatment and faster resolution. In this paper we evaluated the performance of a new Point Of Care Test (POCT) for the rapid prediction of antimicrobial susceptibility in urine samples performed without the need of a laboratory or specialized technicians. 349 patients were enrolled in two open-label, monocentric, non-interventional clinical trials in partnership with an Emergency Medicine ward and the Day Hospital of two large healthcare facilities in Rome. Antibiogram was carried out on 97 patients. Results from analysis of urine samples with the POCT were compared with those from routine AST performed on culture-positive samples, displaying high accuracy (>90%) for all tested antimicrobial drugs and yielding reliable results in less than 12 hours from urine collection thus reducing analytical and management costs.

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A new device for the prompt diagnosis of urinary tract infections

Abstract: The results obtained demonstrate that the MBS point-of-care testing (POCT) device could be developed into a valuable aid for the management of UTIs, possibly addressing more precise diagnosis and appropriate therapy.

Cited by 4 publications

References 14 publications

Management of Urinary Tract Infections: Problems and Possible Solutions

Management of Urinary Tract Infections: Problems and Possible Solutions

Integrating running water monitoring tools with the Micro Biological Survey (MBS) method to improve water quality assessment

A new point-of-care test for the rapid antimicrobial susceptibility assessment of uropathogens

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