A new dimeric phenylpropanoid and cytotoxic norditerpene constituents fromPodocarpus nakaii

Abstract

A new dimeric phenylpropanoid namely podonaka A (1), along with the 13 known compounds including diterpenes (2 and 3), norditerpenes (4 and 5), benzenoids (6-10), steroids (11 and 12), chalcone (13), and megastigmane (14), was isolated from the EtOH extract of Podocarpus nakaii Hayata. The structure of 1 was elucidated on the basis of spectroscopic analysis including 1D and 2D NMR and MS techniques. Biological evaluation showed that norditerpenes, inumakilactone B (4), and podolactone E (5) have potent cytotoxic activities against Daoy, WiDr, KB, and HeLa tumor cell lines. Moreover, low dosage of 5 may induce early apoptosis in KB cells before 12 h.

Anticancer Activities and Mechanism of Action of Nagilactones, a Group of Terpenoid Lactones Isolated from Podocarpus Species

“…In the same study, Nag-F and Nag-G were found to be more cytotoxic than Nag-C, with IC 50 around or below 1 mM against the same human cancer cell lines [26]. The structurally related compounds inumakilactone B and podolactone E were equally potent at inhibiting the growth of different cancer cell lines in vitro, with IC 50 in the 1-5 mM range also [27]. Not all nagilactones are cytotoxics.…”

Isolation of 16 polymorphic microsatellite markers from an endangered and endemic species, Podocarpus nakaii (Podocarpaceae)

“…Currently, human activities over the past 20 years in central Taiwan have destroyed the habitat of P. nakaii to create space for fruit orchards. Recently, several natural products have been isolated from P. nakaii , including dimeric phenylpropanoids, diterpenes, norditerpenes, benzenoids, steroids, chalcones, and megastigmanes (Huang et al, 2009). Studies have demonstrated that these phytochemicals have anticancer potential in several human tumor cell lines and may be used in anticancer therapies (Huang et al, 2009).…”

“…Recently, several natural products have been isolated from P. nakaii , including dimeric phenylpropanoids, diterpenes, norditerpenes, benzenoids, steroids, chalcones, and megastigmanes (Huang et al, 2009). Studies have demonstrated that these phytochemicals have anticancer potential in several human tumor cell lines and may be used in anticancer therapies (Huang et al, 2009). Nevertheless, the genetic diversity and the population structure of P. nakaii remain unclear.…”