A new DNA sensor design for the simultaneous detection of HPV type 16 and 18 DNA

Jampasa, Sakda; Siangproh, Weena; Laocharoensuk, Rawiwan; Yanatatsaneejit, Pattamawadee; Vilaivan, Tirayut; Chailapakul, Orawon

doi:10.1016/j.snb.2018.03.045

Cited by 54 publications

(35 citation statements)

References 34 publications

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“…This section critically summarizes the electrochemical approaches which utilized the aptamers as bio-recognition element towards the detection of viruses, such as HAV ( Manzano et al, 2018 ), HBV ( Campos-Ferreira et al, 2013 ; Jampasa et al, 2018 , 2014 ; Karimizefreh et al, 2014 ; Qian et al, 2020 ; Tran et al, 2011 ), HCV ( Ghanbari et al, 2017 ; Ghanbari and Roushani, 2018 ; S. Liu et al, 2011 ), HPV( Bartosik et al, 2016 ; Campos-Ferreira et al, 2016 ; Civit et al, 2012 ; Huang et al, 2015 ; Souza et al, 2014 ), HIV ( Chen et al, 2012 ; Fatin et al, 2019 ; Guo et al, 2013 ; Radhi et al, 2016 ; Rahim Ruslinda et al, 2013 ; Wang et al, 2015 ), Zika( Aydinlik et al, 2011 ; Dolai and Tabib-azar, 2020 ; Faria and Zucolotto, 2019 ; Moço et al, 2019 ; Steinmetz et al, 2019 ), Dengue ( Rai et al, 2012 ), Chikungunya ( Singhal et al, 2018b , 2018a ), Avian ( Dong et al, 2015 ; X. Liu et al, 2011 ), Influenza ( Tam et al, 2009 ; Yamanaka et al, 2011 ) and Hepatitis( Cho et al, 2018 ; Li et al, 2015 ).…”

Section: Electrochemical Diagnosticsmentioning

confidence: 99%

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Electrochemical diagnostics of infectious viral diseases: Trends and challenges

Goud

Reddy

Khorshed

et al. 2021

Biosensors and Bioelectronics

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Infectious diseases caused by viruses can elevate up to undesired pandemic conditions affecting the global population and normal life function. These in turn impact the established world economy, create jobless situations, physical, mental, emotional stress, and challenge the human survival. Therefore, timely detection, treatment, isolation and prevention of spreading the pandemic infectious diseases not beyond the originated town is critical to avoid global impairment of life (e.g., Corona virus disease - 2019, COVID-19). The objective of this review article is to emphasize the recent advancements in the electrochemical diagnostics of twelve life-threatening viruses namely - COVID-19, Middle east respiratory syndrome (MERS), Severe acute respiratory syndrome (SARS), Influenza, Hepatitis, Human immunodeficiency virus (HIV), Human papilloma virus (HPV), Zika virus, Herpes simplex virus, Chikungunya, Dengue, and Rotavirus. This review describes the design, principle, underlying rationale, receptor, and mechanistic aspects of sensor systems reported for such viruses. Electrochemical sensor systems which comprised either antibody or aptamers or direct/mediated electron transfer in the recognition matrix were explicitly segregated into separate sub-sections for critical comparison. This review emphasizes the current challenges involved in translating laboratory research to real-world device applications, future prospects and commercialization aspects of electrochemical diagnostic devices for virus detection. The background and overall progress provided in this review are expected to be insightful to the researchers in sensor field and facilitate the design and fabrication of electrochemical sensors for life-threatening viruses with broader applicability to any desired pathogens.

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Section: Electrochemical Diagnosticsmentioning

confidence: 99%

“…Finally, ASD methodology applied for the simultaneous detection of two high-risk human papillomavirus DNA sequences. The calculated detection limits were 150 and 153 pM, for HPV type 16 and 18 sequences respectively in the concentration range of 0.5 - 100 nM ( Jampasa et al, 2018 ).…”

Section: Electrochemical Diagnosticsmentioning

confidence: 99%

Electrochemical diagnostics of infectious viral diseases: Trends and challenges

Goud

Reddy

Khorshed

et al. 2021

Biosensors and Bioelectronics

View full text Add to dashboard Cite

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“…In general, DNA biosensors present many advantages such as rapid detection, low cost, portable, good sensitivity and selectivity, compatible with mass production, and less complicated to use compared to other nucleic acid assays 20 . Jampasa et al (2018), develop and fabricate a new electrochemical DNA sensor based on sandwich hybridization to detect HPV-16 and 18 simultaneously. The sensor contains two signaling probes, the first one is an unlabeled acpcPNA capture probe called P1.…”

Section: Novel Electrochemical Dna Biosensor For the Detection Of Hpvmentioning

confidence: 99%

Prevention of cervical cancer development through early detection of HPV using novelty molecular applications

León-Ordóñez

Abad-Sojos

2019

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Human Papillomavirus (HPV) is the predominant cause of cervical cancer worldwide. The infections with HPVs 16 and 18 have a high oncogenic risk for cancer development. Besides, the genes E6 and E7 encode viral oncoproteins associated with infection. New molecular techniques for HPV detection, show important advantages such as high sensitivity, recognition capacity, reliability, among others. These techniques allow the standardization of new protocols associated with the detection in a variety of substances and samples. The stretch relationship between the virus and the disease open a new field to study early detection of the HPV infection. Additionally, less concentration of the sample is needed. Considering the significance of the detection, the present paper explains five novelty molecular applications for the prevention cervical cancer and early detection of HPV such as RNA in situ Hybridization for the detection of HPV E6/E7, genosensors, electrochemical DNA biosensor, PCR-based urine assay and a semen assay for detection of HPV. All the methods related to DNA samples could be used for both genders, there are more acceptable and easy to collect.

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“…Simultaneous electrochemical sensing of multiple species without pretreatment can also be performed using chemically modified electrodes (CMEs). [28][29][30][31] CMEs and arrays of CMEs have been incorporated into ePADs for multiplexed detection of cancer biomarkers, [32,33] heavy metals in environmental [34] and human serum samples, [35] and an array of other environmental and biological analytes. Still, the electrochemical experiments typically take place under a single set of conditions, including pH, ionic strength, and solvent.…”

Section: Introductionmentioning

confidence: 99%

Janus electrochemistry: Simultaneous electrochemical detection at multiple working conditions in a paper-based analytical device

Nantaphol

Kava

Channon

et al. 2019

Analytica Chimica Acta

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The simultaneous detection of multiple analytes from a single sample is a critical tool for the analysis of real world samples. However, this is challenging to accomplish in the field by current electroanalytical techniques, where tuning assay conditions towards a target analyte often results in poor selectivity and sensitivity for other species in the mixture. In this work, an electrochemical paper-based analytical device (ePAD) capable of performing simultaneous electrochemical experiments in different solution conditions on a single sample was developed for the first time. We refer to the system as a Janus-ePAD after the two-faced Greek god because of the ability of the device to perform electrochemistry on the same sample under differing solution conditions at the same time with a single potentiostat. In a Janus-ePAD, a sample wicks down two channels from a single inlet towards two discreet reagent zones that adjust solution conditions, such as pH, before flow termination in two electrochemical detection zones. These zones feature independent working electrodes and shared reference and counter electrodes, facilitating simultaneous detection of multiple species at each species' optimal solution condition. The device utility and applicability are demonstrated through the simultaneous detection of two biologically relevant species (norepinephrine and serotonin) and a common enzymatic assay product (p-aminophenol) at two different solution pH conditions. Janus-ePADs show great promise as an inexpensive and broadly applicable platform which can reduce the complexity and/or number of steps required in *

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A new DNA sensor design for the simultaneous detection of HPV type 16 and 18 DNA

Cited by 54 publications

References 34 publications

Electrochemical diagnostics of infectious viral diseases: Trends and challenges

Electrochemical diagnostics of infectious viral diseases: Trends and challenges

Prevention of cervical cancer development through early detection of HPV using novelty molecular applications

Janus electrochemistry: Simultaneous electrochemical detection at multiple working conditions in a paper-based analytical device

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