A new electrochemical DNA biosensor based on modified carbon paste electrode using graphene quantum dots and ionic liquid for determination of topotecan

Mahmoudi–Moghaddam, Hadi; Tajik, Somayeh; Beitollahi, Hadi

doi:10.1016/j.microc.2019.104085

Cited by 119 publications

(44 citation statements)

References 40 publications

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“…In this work, an on-chip sensing system was exploited for p16 quantification through various electrochemical methods like DPV and EIS and were characterized by employing several other techniques such as TEM, SEM and UV-Vis spectroscopy to develop a facile DNA hybridization sensor (Figure 16) for the early diagnosis of pancreatic cancer, which possessed an LOD of 0.10 pM. In 2019, Mahmoudi-Moghaddam et al provided an electrochemical strategy for topotecan detection by investigating topotecan-DNA interaction on carbon paste electrode (CPE) [269]. The biosensor was constructed by functionalizing a CPE with ionic liquid (IL), GQDs and doublestranded DNA (ds-DNA).…”

Section: Voltammetric Gqd-sensorsmentioning

confidence: 99%

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Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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Section: Voltammetric Gqd-sensorsmentioning

confidence: 99%

“…In 2019, Mahmoudi-Moghaddam et al provided an electrochemical strategy for topotecan detection by investigating topotecan-DNA interaction on carbon paste electrode (CPE) [269]. The biosensor was constructed by functionalizing a CPE with ionic liquid (IL), GQDs and double-stranded DNA (ds-DNA).…”

Section: Voltammetric Gqd-sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

190

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“…Topotecan is used as an antineoplastic agent in the therapy of cervical, colorectal, ovarian, and small cell lung cancer, acting through the inhibition of topoisomerase I activity. Topotecan electrochemical detection with a DNA electrochemical biosensor, based on dsDNA immobilization onto a CPE modified by ionic liquid and graphene quantum dots, was investigated [ 71 ]. Topotecan detection by DPV, in the linear range 0.35–100.0 μM, with an LOD = 0.1 μM, was obtained.…”

Section: Dna Electrochemical Biosensors For the Detection Of Pharmmentioning

confidence: 99%

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Chiorcea-Paquim

Oliveira-Brett

2021

Sensors

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Deoxyribonucleic acid (DNA) electrochemical biosensors are devices that incorporate immobilized DNA as a molecular recognition element on the electrode surface, and enable probing in situ the oxidative DNA damage. A wide range of DNA electrochemical biosensor analytical and biotechnological applications in pharmacology are foreseen, due to their ability to determine in situ and in real-time the DNA interaction mechanisms with pharmaceutical drugs, as well as with their degradation products, redox reaction products, and metabolites, and due to their capacity to achieve quantitative electroanalytical evaluation of the drugs, with high sensitivity, short time of analysis, and low cost. This review presents the design and applications of label-free DNA electrochemical biosensors that use DNA direct electrochemical oxidation to detect oxidative DNA damage. The DNA electrochemical biosensor development, from the viewpoint of electrochemical and atomic force microscopy (AFM) characterization, and the bottom-up immobilization of DNA nanostructures at the electrode surface, are described. Applications of DNA electrochemical biosensors that enable the label-free detection of DNA interactions with pharmaceutical compounds, such as acridine derivatives, alkaloids, alkylating agents, alkylphosphocholines, antibiotics, antimetabolites, kinase inhibitors, immunomodulatory agents, metal complexes, nucleoside analogs, and phenolic compounds, which can be used in drug analysis and drug discovery, and may lead to future screening systems, are reviewed.

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“…Deposition of a PyO solution on the modified electrode Phosphate [122] DNA/GQD/IL/CPE ds-DNA Electrodeposition of ds-DNA, 0.5 mol L À 1 acetate buffer with a potential of + 0.5 V, mixing for 250 s Topotecan [123] DNA/AuNPs-rGO DNA 5'-SH-TTTTCGCTTTT-3'…”

Section: Graphene-based Materialsmentioning

confidence: 99%

Structure, Properties, and Electrochemical Sensing Applications of Graphene‐Based Materials

et al. 2020

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Owing to their versatility and unique characteristics, graphene‐based materials have been used extensively for the development of electrochemical sensors and biosensors. The key to the maximum potential of these materials is the understanding of the role their structure plays in their modification processes. Herein, we summarize some structural characteristics of graphene oxide (GO) and reduced graphene oxide (rGO) and explore different surface modification methods for electrochemical sensing applications. surveyed the most recent applications of these materials as (bio)sensors, particularly for environmental monitoring and health‐related applications, such as quantification of biomarkers and metabolites and detection of cancer cells. The low detection limits, selectivity toward target molecules, and robustness of GO‐ and rGO‐based electrodes render them critical materials for the preparation of sensors for routine analysis and monitoring.

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A new electrochemical DNA biosensor based on modified carbon paste electrode using graphene quantum dots and ionic liquid for determination of topotecan

Cited by 119 publications

References 40 publications

Applications of Graphene Quantum Dots in Biomedical Sensors

Applications of Graphene Quantum Dots in Biomedical Sensors

DNA Electrochemical Biosensors for In Situ Probing of Pharmaceutical Drug Oxidative DNA Damage

Structure, Properties, and Electrochemical Sensing Applications of Graphene‐Based Materials

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