A new enzyme-linked immunosorbent assay (ELISA) for measuring immunoconglutinins directed against the third component of human complement. Findings in systemic lupus erythematosus

Durand, Charles G.; Burge, J

doi:10.1016/0022-1759(84)90031-0

Cited by 22 publications

(14 citation statements)

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“…The titers of immunoconglutinins decreased with the achievement of remission in patients with steroid-sensitive nephrotic syndrome (16). The anti-C3 autoantibody titers, measured by ELISA, in a SLE cohort (11,13) and in the DDD patients (7) also decreased with improvement of the clinical condition and normalization of the C3 levels. In agreement with these results, our data indicate an association of the level of C3-binding antibodies with the disease activity.…”

Section: Discussionmentioning

confidence: 99%

“…C3 is a convergent point of the complement system, common between the classical, lectin, and alternative pathways. Antibodies against C3 have been described in patients with autoimmune diseases, such as SLE (11)(12)(13)(14) or Crohn disease (15), in some nephrotic kidney diseases (16,17), and in dense deposit disease (DDD) (7) as well as in autoimmune-prone mice (12). Based on the limited functional studies of these antibodies, it is difficult to conclude whether they are a disease-relevant factor or a simple epiphenomenon, one among many, arising from the dysregulated immune response in the autoimmune diseases (18,19).…”

Section: Lupus Nephritis (Ln)mentioning

confidence: 99%

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Functional Characterization of Autoantibodies against Complement Component C3 in Patients with Lupus Nephritis

Vasilev

Noé

Dragon-Durey

et al. 2015

Journal of Biological Chemistry

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Background: Autoantibodies against complement C3 are found in patients with the autoimmune disease systemic lupus erythematosus. Results: C3 autoantibodies are found in 30% of lupus nephritis patients and inhibit C3 interaction with the regulatory proteins Factor H and CR1. Conclusion: C3 autoantibodies have overt capability to overactivate complement. Significance: C3 autoantibodies can contribute to the pathological process in lupus nephritis.

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Section: Discussionmentioning

confidence: 99%

Section: Lupus Nephritis (Ln)mentioning

confidence: 99%

Functional Characterization of Autoantibodies against Complement Component C3 in Patients with Lupus Nephritis

Vasilev

Noé

Dragon-Durey

et al. 2015

Journal of Biological Chemistry

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“…They have been reported to occur in high levels in patients with SLE (15)(16)(17). No reports have shown a specific association of anti-C3b antibody with LN.…”

Section: Discussionmentioning

confidence: 99%

Relationship of Circulating Anti-C3b and Anti-C1q IgG to Lupus Nephritis and Its Flare

Birmingham

Bitter

Ndukwe

et al. 2016

Clinical Journal of the American Society of Nephrology

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Background and objectives Autoantibodies to complement C1q (anti-C1q) are associated with the diagnosis of lupus nephritis. In this study, we compare anti-C1q IgG with another complement autoantibody, anti-C3b IgG, as a biomarker of lupus nephritis and lupus nephritis flare.Design, setting, participants, & measurements Our investigation involved the Ohio SLE Study, a prospective observational cohort of patients with recurrently active lupus who were followed bimonthly. Serum anti-C1q and anti-C3b IgG levels were assessed cross-sectionally by ELISA in 40 normal controls and 114 patients in the Ohio SLE Study (41 nonrenal and 73 lupus nephritis) at study entry, and longitudinally in a subset of patients in the Ohio SLE Study with anti-C1q-positive lupus nephritis in samples collected every 2 months for 8 months leading up to lupus nephritis flare (n=16 patients). ResultsIn the cross-sectional analysis, compared with anti-C1q IgG, anti-C3b IgG was less sensitive (36% versus 63%) but more specific (98% versus 71%) for lupus nephritis. Only anti-C3b IgG was associated with patients with lupus nephritis who experienced at least one lupus nephritis flare during the Ohio SLE Study period (P,0.01). In the longitudinal analysis, circulating levels of anti-C1q IgG increased at the time of lupus nephritis flare only in patients who were anti-C3b positive (P=0.02), with significant increases occurring from 6 (38% increase) and 4 months (41% increase) before flare. Anti-C3b IgG levels also trended up at lupus nephritis flare, although the change did not reach statistical significance (P=0.07). Neither autoantibody increased 2 months before flare.Conclusions Although not as prevalent as anti-C1q IgG, anti-C3b IgG showed nearly complete specificity for lupus nephritis. The presence of anti-C3b IgG identified patients with lupus nephritis who were prone to flare and in whom serial measurements of markers associated with complement, such as anti-C1q IgG, may be useful to monitor lupus nephritis activity.

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“…By using agglutination techniques, IKs have been reported to be mainly of IgM class, but, in addition, both IgG and IgA class have been detected [1]. Elevated levels of IgG IK have predominantly been demonstrated by ELISA [2]. IKs increase in response to acute or chronic inflammations involving complement activation, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…IKs increase in response to acute or chronic inflammations involving complement activation, e.g. infections, and a variety of diseases of autoimmune origin [ 1,3] such as Crohn's disease, rheumatoid arthritis (RA) [4] and SLE [2].…”

Section: Introductionmentioning

confidence: 99%

Detection and characterization of immunoconglutinins in patients with systemic lupus erythematosus (SLE): serial analysis in relation to disease course

Nilsson

Ekdahl

Sjöholm

et al. 1992

Clinical and Experimental Immunology

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SUMMARYThe levels of IgA. IgG and IgM immunoconglulinins (IK) were assessed in sera from 20 palienls wilh SLE which were followed for 8-monlh periods. Al the time of the exacerbation, IgG IKs were significantly increased to 226 + 90 arbitrary units (mean±s.e.m.) compared wilh both the minimum value of 75 + 28 in the SLE patients and with 31+2 in healthy controls (/*<005). There was no difference between SLE patients and controls in the levels of IgM and IgA IKs. Most of the SLE patients in this material showed maximal IgG IK levels before exacerbation, but there was no correlation between the clinical disease index and the levels of IgG IK. The specificity of IgG IKs showed a broad diversity for microtit re-fixed C3b, iC3b. C3c and C3dg. The antibodies were of IgG 1, IgG3 and in two patients. lgG4 subclass. IgG IKs were correlated to the C3d/C3 ratio which suggested that the IK responses were secondary to C3 activation. In summary, unlike other conditions associated with complement activation where elevated IgM 1 Ks are common, an increase in IgG IK levels was observed. It is possible that this diverging IK response contributes to the pathophysiology of the disease.

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A new enzyme-linked immunosorbent assay (ELISA) for measuring immunoconglutinins directed against the third component of human complement. Findings in systemic lupus erythematosus

Cited by 22 publications

References 12 publications

Functional Characterization of Autoantibodies against Complement Component C3 in Patients with Lupus Nephritis

Functional Characterization of Autoantibodies against Complement Component C3 in Patients with Lupus Nephritis

Relationship of Circulating Anti-C3b and Anti-C1q IgG to Lupus Nephritis and Its Flare

Detection and characterization of immunoconglutinins in patients with systemic lupus erythematosus (SLE): serial analysis in relation to disease course

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