A new era for plant biotechnology

Hunter, Philip

doi:10.1038/embor.2011.91

Cited by 3 publications

(3 citation statements)

References 3 publications

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“…Different plant tissues such as leaves, seeds and tubers have been engineered for producing useful recombinant proteins. The production of therapeutic proteins in seeds offers one of the most appealing efficient systems as a target tissue . Seeds naturally store stable proteins for long periods of time.…”

Section: Introductionmentioning

confidence: 99%

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Expression of cholera toxin B subunit–lumbrokinase in edible sunflower seeds–the use of transmucosal carrier to enhance its fusion protein's effect on protection of rats and mice against thrombosis

Guan

Chen

et al. 2014

Biotechnology Progress

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Lumbrokinase (LK) is a group of serine proteases with strong fibrinolytic and thrombolytic activities and is useful for treating diseases caused by thrombus. Cholera toxin B subunit (CTB) has been widely used to facilitate antigen delivery by serving as an effective mucosal carrier molecule for the induction of oral tolerance. We investigate here the application of CTB as a transmucosal carrier in enhancing its fusion protein-LKs effect to protect rats against thrombosis. Thus, in this study, CTB-LK fusion gene separated by a furin cleavage site was expressed in seeds of Helianthus annuus L. The activity of recombinant protein in seeds of transgenic sunflower was confirmed by Western blot analysis, fibrin plate assays and GM1 -ganglioside ELISA. The thrombosis model of rats and mice revealed that the oral administration of peeled seeds of sunflower expressing CTB-LK had a more significant anti-thrombotic effect on animals compared with that administration of peeled seeds of sunflower expressing LK. It is possible to conclude that CTB can successfully enhance its fusion protein to be absorbed in rats or mice thrombosis model. The use of CTB as a transmucosal carrier in the delivery of transgenic plant-derived oral therapeutic proteins was supported. In addition, for the purpose of that recombinant CTB-LK was designed for oral administration, thus the expression of CTB-LK in edible sunflower seeds eliminated the need for downstream processing of proteins.

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Section: Introductionmentioning

confidence: 99%

“…The production of therapeutic proteins in seeds offers one of the most appealing efficient systems as a target tissue. 21 Seeds naturally store stable proteins for long periods of time. Furthermore, the major proportion of seed proteins belongs to a limited set of protein classes, which may simplify the purification procedure.…”

Section: Introductionmentioning

confidence: 99%

Expression of cholera toxin B subunit–lumbrokinase in edible sunflower seeds–the use of transmucosal carrier to enhance its fusion protein's effect on protection of rats and mice against thrombosis

Guan

Chen

et al. 2014

Biotechnology Progress

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show abstract

“…In addition, progress in plant breeding and genetic engineering techniques put constant challenges to current risk assessment approaches and the sustainable use of GM plants (e.g. COGEM, 2010, Cominelli and Tonelli, 2010, Fedoroff, 2010, Jaggard et al, 2010, Llorente et al, 2010, Piesse and Thirtle, 2010, Franke et al, 2011, Hunter, 2011.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Overview Studies to evaluate existing Research and knowledge on biological issues on GM Plants of relevance to Swiss Environments

Sweet¹,

Bartsch²

2012

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Involvement of Various Biotechnological Contrivances for Tropane Alkaloid Biosynthesis and Applications of Tropane Alkaloid-Bearing In Vitro Cultures

et al. 2021

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A new era for plant biotechnology

Abstract: New applications and technologies, and more rigorous safety measures could herald a new era for genetically modified crops with improved traits, for use in agriculture and the pharmaceutical industry.

Cited by 3 publications

References 3 publications

Expression of cholera toxin B subunit–lumbrokinase in edible sunflower seeds–the use of transmucosal carrier to enhance its fusion protein's effect on protection of rats and mice against thrombosis

Expression of cholera toxin B subunit–lumbrokinase in edible sunflower seeds–the use of transmucosal carrier to enhance its fusion protein's effect on protection of rats and mice against thrombosis

Synthesis and Overview Studies to evaluate existing Research and knowledge on biological issues on GM Plants of relevance to Swiss Environments

Involvement of Various Biotechnological Contrivances for Tropane Alkaloid Biosynthesis and Applications of Tropane Alkaloid-Bearing In Vitro Cultures

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