A new future of forensic Y-chromosome analysis: Rapidly mutating Y-STRs for differentiating male relatives and paternal lineages

Ballantyne, Kaye N.; Keerl, Victoria; Wollstein, Andreas; Choi, Ying; Zuñiga, Sofia; Ralf, Arwin; Vermeulen, Mark; Knijff, Peter de; Kayser, Manfred

doi:10.1016/j.fsigen.2011.04.017

Cited by 229 publications

(170 citation statements)

References 41 publications

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“…Such is the case of locus DYS570, which in PPY23 is amplified by means of PCR primers giving rise to amplicons which are decidedly shorter (90-146 bp) [25] than those obtained with the present protocol (246-286 bp) [5], and therefore less prone to the potential effects of DNA degradation.…”

Section: Discussionmentioning

confidence: 83%

“…A systematic Y-STR mutation study by Ballantyne et al [3] identified a set of 13 novel "rapidly mutating" (RM) Y-STR markers with exceptionally high mutation rates (>10 -2 per locus per generation) if compared to >170 other Y-STRs including all conventionally used markers, the latter in the order of a few mutations per marker every 1,000 generations [4]. RM Y-STRs have been demonstrated not only to improve the resolution of male lineage differentiation, but also to allow close male relative separation with a power unsurpassed by standard Y-STRs [5].…”

mentioning

confidence: 99%

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Development of an Italian RM Y-STR haplotype database: Results of the 2013 GEFI collaborative exercise

Robino

Ralf

Pasino

et al. 2015

Forensic Science International: Genetics

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Section: Discussionmentioning

confidence: 83%

mentioning

confidence: 99%

Development of an Italian RM Y-STR haplotype database: Results of the 2013 GEFI collaborative exercise

Robino

Ralf

Pasino

et al. 2015

Forensic Science International: Genetics

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“…A set of Y-STR loci with mutation rates above 0.01 was recently described [6]. These rapidly mutating Y-STRs (RM Y-STRs) are very useful for the discrimination between closely related and unrelated males [6,7]. RM Y-STRs are included in the latest generation of commercial Y-STR multiplex kit.…”

Section: Introductionmentioning

confidence: 99%

Forensic and population genetic analyses of Danes, Greenlanders and Somalis typed with the Yfiler ® Plus PCR amplification kit

Olofsson

Mogensen

Buchard

et al. 2015

Forensic Science International: Genetics

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“…25 Brothers should have the same Y-STR type, as is the case for most STRs on the Y chromosome, but some of these rapidly mutating loci may be different, allowing even brothers to be differentiated by Y-STR typing. 23,26 X-STR typing STR loci on the X chromosome are very new and have a limited but valuable niche.…”

Section: Y-str Typingmentioning

confidence: 99%

Forensic DNA profiling: state of the art

Linacre¹,

Templeton²

2014

RRFMS

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DNA profiling is now a routine test, yet it has only been 26 years since its first use. During these intervening years, the science of DNA profiling has undergone many changes, and it will continue to do so in the future. DNA profiling in the laboratory starts with DNA extractions and is followed by quantification of the DNA, amplification of up to 23 human specific short tandem repeat loci, and separation on capillary columns. The final stage is interpretation of the data leading to reporting the DNA evidence to a court. Each of these steps has seen improvements leading to higher sensitivity from minute trace samples. Genetic markers on the X and Y chromosomes are now used for both criminal and civil investigations, along with mitochondrial DNA. Mitochondrial DNA has been used in forensic science since the 1990s because of its high copy number and particular maternal inheritance. These sex chromosome markers and mitochondrial DNA were used effectively to determine the identity or ancestry of individuals, and now other genetic markers can be used to determine certain phenotypic traits to a high degree of accuracy. Genetic testing adapted from medical and pharmaceutical sciences, such as next-generation DNA sequencing, will soon be applied to mainstream forensic science, opening new avenues in criminal investigations. This review aims to cover these key events and advancements in the field from both a historical view and current practice. Keywords: forensic science, DNA profiling, short tandem repeats, mitochondrial DNA, phenotypic testing, next-generation sequencing Opening commentsDNA profiling has revolutionized the process of forensic human identification. It is of note that this is a relatively recent addition to the forensic science tool kit and that it is constantly undergoing developments. The aim of this review is to take the reader through the processes of DNA typing, starting with DNA isolation from biological material through novel approaches and phenotypic testing. The review is written for those in forensic medicine; therefore, certain knowledge is assumed, but equally, aspects that are specific to forensic science will be emphasized and placed into the right context. For those who wish more information than can be provided within the scope of this review, there are books ranging from introductory texts 1 to those that are more comprehensive.2,3

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A new future of forensic Y-chromosome analysis: Rapidly mutating Y-STRs for differentiating male relatives and paternal lineages

Cited by 229 publications

References 41 publications

Development of an Italian RM Y-STR haplotype database: Results of the 2013 GEFI collaborative exercise

Development of an Italian RM Y-STR haplotype database: Results of the 2013 GEFI collaborative exercise

Forensic and population genetic analyses of Danes, Greenlanders and Somalis typed with the Yfiler ® Plus PCR amplification kit

Forensic DNA profiling: state of the art

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