A new gastric carcinoma cell line (LIM1839) derived from a young caucasian male

Abstract

We describe a new and unique gastric carcinoma cell line (LIM1839) derived from a young Caucasian male with rapidly progressing disease. The cell line grows with a pleomorphic morphology and has been in continuous culture for more than 3 years. The cells cannot be cloned in semi-solid agar or grown in nude mice despite numerous attempts. The karyotype of the cultured cells is highly abnormal with a large number of structural and numerical changes. Some chromosomes are dicentric and this feature has persisted in this culture. The cells express one of the small-intestinal dipeptidases, aminopeptidase N, but do not express dipeptidyl peptidase IV or the disaccharidases, sucrase isomaltase or maltase glucoamylase. The cells express high levels of EGF receptors and of messenger RNA for insulin-like growth factor II.

Expression of MUC1 and MUC2 Mucins by Human Tumor Cell Lines

“…The pro duction of the LIM cell lines, many of which arc struc turally and functionally unique, has been described [18][19][20][21]. All other cell lines were obtained from ATTC, except HT29-SB, which is a mucin-secreting deriva tive of HT29 produced by sodium butyrate induction [9].…”

“…All other cell lines were obtained from ATTC, except HT29-SB, which is a mucin-secreting deriva tive of HT29 produced by sodium butyrate induction [9]. The LIM cell lines were maintained in RPMI-1640 (ICN Biomcdicals, Australia) containing 5 % fetal calf serum (FCS; ICN Biomedical, Australia), while the other cell lines were maintained in Iscove's medium (Pacific Diagnostics, Australia) containing 8% FCS [9, [18][19][20]. Culture supernatants from confluent cultures were concentrated 10-fold in an Ultrafree-CL 10,000 molecular weight cutoff filter unit (Millipore, USA).…”

Establishment and characterization of a cisplatin-resistant cell line (IGSK-1) from a poorly differentiated gastric adenocarcinoma

“…The chromosome number is shown in Table 1. G-band karyotypes of IGSK-1 were 77-85 [4n], XXX, −X[10], −1 [10], add(1)(p11) [9], −2 [4], −4 [10], del(4)(q?) [5], −5 [3], add(6)(q13) [8], +7 [7], add(8)(p11) [10], add(8)(p21) [8], inv(9)(p11q13) × 2 [10], −10 [10], +13 [2], −13 [6], −14 [10], −14 [4], −15 [10], −16 [10], add(16)(p13) × 2 [10], Journal compilation © 2007 Japan Human Cell Society add(16)(p13) [10], −17 [4], add(17)(q21) [10], add(16)(p13) [10], +18 [8], +18 [3], add(18)(q21) [8], −19 [9], add(19)(p13) [2], −20 [10], −20 [7], −21 [10], −22 [10], +mar1 [9], +0-4mar, 1-15dmin as shown in Fig.…”

“…There are many reports on the establishment of cell lines derived from original, metastatic foci or recurrent gastric tumors, [1][2][3][4][5][6][7][8][9][10][11][12][13] including: the TMK-1 and MKN-45 cell lines derived from poorly differentiated adenocarcinoma; MKN-1 derived from adenosquamous carcinoma; MKN-7 and MKN-28 cell lines derived from well differentiated adenocarcinoma, MKN-74 derived from moderately differentiated tubular adenocarcinoma; KATO-III and HSC-39 derived from signet ring cell carcinoma; 12 and HSC-44 PE and HSC-58 derived from scirrhous carcinoma. 13 However, this is the first report of the establishment of a gastrin and serotonin secreting and serotonin incorporating cell line derived from gastric poorly differentiated adenocarcinoma.…”

Establishment and characterization of an IGSK-2 cell line derived from ascitic fluid of recurrent hCG and somatostatin secreted adenocarcinoma of the stomach

“…There are several reports on the establishment of cell lines derived from stomach cancer 1–11 . As stomach cancer is a relatively common disease in Japan, the chemotherapeutic approach is very common and useful.…”