A new gentiopicroside derivative improves cognitive deficits of AD mice via activation of Wnt signaling pathway and regulation of gut microbiota homeostasis

Wang, Jianyu; Fasina, Opeyemi B.; Manzoor, Majid; Wang, Ying; Liu, Qian; Mo, Jian‐Xia; Ohno, Hiroshi; Osada, Hiroyuki; Xiang, Lan; Qi, Jianhua

doi:10.1016/j.phymed.2023.154730

Cited by 11 publications

(3 citation statements)

References 43 publications

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“…Another derivative of gentiopicroside namely 2H-gentiopicroside has been reported to exhibit improvements in the symptoms of mice with Alzheimer's disease. The effect was regulated by the combination of the microbiota-gut brain axis and the Wnt signaling pathway [44]. Gentiopicroside is also reported to possess a potent effect against acute pancreatitis.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics‐based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.‐Ham. ex C.B. Clarke from the western Himalayas

Tewari,

Bawari,

Mishra

et al. 2024

J of Separation Science

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Swertia species are common ingredients in numerous herbal remedies. It is also used to treat a wide range of illnesses and possess diverse therapeutic activities. The aim of the study is to elucidate the comprehensive metabolomics profile of Swertia chirayita and the role of various extraction methods in the phytochemical compositions of the extracts of S. chirayita, and their antioxidant and enzyme inhibitory activities. Extraction of the stems, leaves, and flowering tops of S. chirayita was performed by maceration, infusion, and soxhlation using methanol and water as solvent. Extracts were subjected to phytochemical profiling by a liquid‐chromatographic system. Antioxidant and enzyme inhibitory activity was carried out. The metabolomics profiling showed that a diverse range of specialized metabolites were present in the stems and leaves & flowering tops of the plant. All the extracts showed substantial antioxidant and enzyme inhibitory activities further confirmed by molecular docking studies. This study appraised the use of S. chirayita aerial parts as a potential antioxidant and its therapeutic application in various chronic illnesses including Alzheimer's disease, diabetes, and other skin‐related disorders.

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Section: Discussionmentioning

confidence: 99%

Metabolomics‐based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.‐Ham. ex C.B. Clarke from the western Himalayas

Tewari,

Bawari,

Mishra

et al. 2024

J of Separation Science

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“…NORT is a nonrewarding test that assesses rodent cognitive status by calculating DI and RI, indicating the ability to distinguish new and familiar objects, with healthy animals spending more time exploring new objects ( Bengoetxea et al, 2015 ). Transgenic AD animals exhibit prolonged exposure to familiar objects and lower RI compared to wild-type mice ( Zhang et al, 2012 ), with significant reductions in DI and RI compared to the control group ( Wang et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of thymol on oxidative stress and reelin signaling pathway in Alzheimer's disease model

BİTMEZ,

ÇEVRELİ,

KAŞIKÇI

2024

Turkish Journal of Biology

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Background/aim The purpose of this study was to investigate how thymol affects cognitive functions and the levels of MDA, GSH, Aβ 1–42 , ApoE, reelin, and LRP8 in an AD model induced in male Wistar albino rats with the application of D-galactose (D-gal) and aluminum chloride (AlCl 3 ). Materials and methods In this work, 3-month-old male Wistar albino rats were used. Group 1 served as the Control, Group 2 received 0.5 mL/day saline + 0.5 mL/day sunflower oil, Group 3 was administered 200 mg/kg/day AlCl 3 + 60 mg/kg/day D-gal, Group 4 received 30 mg/kg/day thymol, and Group 5 was administered 200 mg/kg/day AlCl 3 + 60 mg/kg/day D-gal + 30 mg/kg/day thymol. At the end of the 10-week experimental period, behavioral and memory tests were performed. GSH and MDA levels were measured in the obtained serum and brain tissue samples, while Aβ 1–42 , ApoE, reelin, and LRP8 levels were measured in brain tissue samples. Statistical analyses were performed using ANOVA test in Graphpad Prism V8.3 program. A p-value <0.05 was considered significant in intergroup analyses. Results When the novel object recognition test (NORT) results were evaluated, the Alzheimer + thymol (ALZ+TYM) group showed a significant increase in the recognition index (RI) and discrimination index (DI) compared to the Alzheimer (ALZ) group at the 24th hour. Thymol reduced working memory errors (WME), reference memory errors (RME), and maze completion time at 48, 72, and 96 hours when evaluated in terms of spatial memory in rats with Alzheimer’s disease. Furthermore, Aβ 1–42 and ApoE levels were increased in the ALZ group compared to the control (C), while reelin and LRP8 levels were decreased in the ALZ group compared to the C group. Conclusion The data we obtained suggest that thymol may play an effective role in cognitive processes against AD and have an anti-Alzheimer’s disease effect.

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“…By acting on the pathway of AGEs, VEGF-A and VEFG-C, GIE seems to allow a rejuvenation of the skin resulting, among other things, in a decrease in redness. It now would be interesting to evaluate the efficacy of GIE on skin around eyes being established [18].…”

Section: Discussionmentioning

confidence: 99%

In vitro, ex vivo and clinical approaches to evaluate the potential effect of Gentiana lutea extract on skin

Berthon,

Cabannes,

Bouton

et al. 2023

Intern J of Cosmetic Sci

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BackgroundDark circles affect subjects of all ages and in all skin types. They can be treated by various methods, particular by topical solutions. This investigation was directed towards exploring the effect of gentiopicroside (GP) on the skin around the eyes. For this, an extract of Gentiana lutea (GIE) containing GP (65% by dry matter) was evaluated on oxidant and angiogenesis parameters using in vitro and ex‐vivo studies. A clinical experimentation was also realized.MethodsThe effect of GIE at different concentrations on antioxidant gene was evaluated in vitro by RT‐qPCR after treatment of NHDF. The effect of 2.93 μg mL−1 GIE on the release of VEGF‐A and VEGF‐C by NHDF was also studied. The effect of 87.9 μg mL−1 GIE was also evaluated on pseudotube formation in a coculture system of normal dermal microvascular endothelial cells (HMVEC‐d)‐NHDF stimulated or not with VEGF as pro‐angiogenic factor. Prior to these assays, preliminary cytotoxicity assays were performed using a standard WST‐8 reduction assay. The expressions of carboxymethyl‐lysine and glyoxalase‐1 were quantified on skin explants topically treated with 147 μg mL−1 GIE in basal and UVA‐irradiated conditions. A clinical study was conducted in 22 subjects using topical twice daily for 14 days on eye area (split‐face application: cream containing 147 μg mL−1 GIE versus placebo). 3D image acquisition and skin colour measurement were performed at D0 and D14.ResultsTreatment of GIE upregulated the gene expression of NFE2L2 and downregulated the expression of CXCL8. GIE targeted AGEs pathways and reduced the formation of pseudotubes. A total of 147 μg mL−1 GIE gel cream significantly reduced significantly the average roughness and relief of the upper eyelid skin as well as the redness of dark circles after 14 days of application.ConclusionBy acting on the pathway of AGEs, VEGF‐A and VEFG‐C, GIE seems to allow a rejuvenation of the skin resulting, among others, in a decrease in redness. It now would be interesting to evaluate the efficacy of GIE on skin around eyes microbiota, antibacterial gentiopicroside property being well‐established.

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A new gentiopicroside derivative improves cognitive deficits of AD mice via activation of Wnt signaling pathway and regulation of gut microbiota homeostasis

Cited by 11 publications

References 43 publications

Metabolomics‐based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.‐Ham. ex C.B. Clarke from the western Himalayas

Metabolomics‐based pharmaceutical evaluation of different parts of Swertia chirayita (Roxb.) Buch.‐Ham. ex C.B. Clarke from the western Himalayas

Effect of thymol on oxidative stress and reelin signaling pathway in Alzheimer's disease model

In vitro, ex vivo and clinical approaches to evaluate the potential effect of Gentiana lutea extract on skin

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