A new green method for indirect determination of ferric ions in biological samples using Ascorbic acid as reducing agent via the development of CFIA system

Abstract

A simple, sensitive, automated and environmentally friendly FI/MZ technique was suggested for the indirect determination of iron (III) as ferric chloride in bulk and biological. These method involve the reduction of Fe (III) to Fe (II) using ascorbic acid as reducing agent, the reduced ion was reacted with 1,10- phenanthroline as a selective organic reagent to form red-orange complex, measured at λ=510 nm. The complexation ratio between matel ion with ligand was 1:3 ratio (M: L). The colored product obeyed Beer’s Law with linear ranges 1-30 and 8-75 μg/mL with detection limits 1.02, 0.28 μg/mL for batch and FIA/MZ methods, respectively. The rate of sampling was 54 sample/h, the %RSD less than 2% and the recovery was about 99%. The developed technique was simply and low cost with high throughput, it provides the use of an aqueous medium as carrier of chemicals in flow system that is nontoxic and causes no pollution, thereby belonging to green chemistry. The proposed procedure can be successfully applied to estimate the content of Iron (III) in biological samples. The results of estimation are satisfactory as compared with those given by a reference method, showed the new method to be accurate and precise at the 95% confidence level.