A new hepatitis B virus vaccine escape mutation in a renal transplant recipient

Sayiner, A. Arzu; Ağca, Harun; Sengonul, Aylin; Celik, Ali; Akarsu, Mesut

doi:10.1016/j.jcv.2006.12.002

Cited by 16 publications

(17 citation statements)

References 18 publications

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“…Ser143Leu substitution is known to cause problems in some diagnostic assays [Wallace et al, 1994;Ireland et al, 2000]. It was also detected in a vaccine-escape HBV infection of a Turkish renal transplant patient [Sayiner et al, 2007]. It was the only codon variation that caused decreased reactivity in the commercial HBsAg assay based on monoclonal antibodies in the presented study (Table III).…”

Section: Discussionmentioning

confidence: 64%

Naturally occurring MHR variants in Turkish patients infected with hepatitis B virus

Sayıner

Özcan

Sengonul

2008

Journal of Medical Virology

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Major B-cell epitopes are located at the major hydrophilic region (MHR) of hepatitis B virus (HBV) surface antigen (HBsAg). The genotypes, subtypes, and naturally occurring amino acid (aa) substitutions of MHR were analyzed in 81 Turkish adult patients (41 inactive HBsAg carriers and 40 patients with chronic hepatitis B) by direct sequencing of the S gene fragment. All the isolates were genotype D according to the phylogenetic analysis. The most common HBsAg subtype was ayw2, followed by ayw3 while one isolate specified ayw4 by encoding Leu127. MHR variants were detected in 22 of the 81 (27.2%) isolates. The prevalence was significantly higher in the chronic hepatitis B group (42.5%) compared to inactive HBsAg carriers (12.2%). Twenty-two samples had a total of 26 amino acid substitutions involving 14 positions. The majority of the patients had a single variation. Most of the amino acid substitutions were located at the HBs1 region of the MHR, while 9 of the 26 were in the classic "a" determinant (aa 124-147). When samples with "a" variants were evaluated by two different commercial HBsAg tests, only the isolate with Ser143Leu variation had a decreased reactivity in the assay using monoclonal antibodies for capture and detection. In conclusion, the findings of the study was in accordance with previous studies showing HBV genotype and subtype homogeneity (genotype D/ayw) in Turkey. Naturally occurring MHR and "a" determinant variants were common, especially among chronic hepatitis B patients. The influence of detected "a" variants on diagnostic assays was limited.

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Section: Discussionmentioning

confidence: 64%

Naturally occurring MHR variants in Turkish patients infected with hepatitis B virus

Sayıner

Özcan

Sengonul

2008

Journal of Medical Virology

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“…In our study, the high titers of anti‐HBs observed in all donations of this group (Table 5) could neutralize the low viral load of HBV virions and eliminate the risk of infectivity except in the case of the escape mutant (T143A) in which the anti‐HBs could be nonneutralizing. The development of HBV infection with occult HBV escape mutants in immunosuppressed patients with pre‐existing anti‐HBs was recently reported 46,47 . The three anti‐HBs–negative donations could be infectious, however.…”

Section: Discussionmentioning

confidence: 99%

“…The development of HBV infection with occult HBV escape mutants in immunosuppressed patients with pre-existing anti-HBs was recently reported. 46,47 The three anti-HBs-negative donations could be infectious, however. The HBV infectivity by anti-HBc-only blood donations have been described, 45,48 but the correlation between the presence of HBV DNA and the infectivity in blood donors is still unclear.…”

Section: Discussionmentioning

confidence: 99%

Detection and characterization of hepatitis B virus of anti‐hepatitis B core antigen–reactive blood donors in Quebec with an in‐house nucleic acid testing assay

et al. 2007

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The 3.25 percent HBV DNA positivity rate among the anti-HBc-reactive donations and more particularly the low level of HBV DNA observed in occult donations underline the importance of the use of a sensitive assay to detect HBV DNA in conjunction with other markers. The HBV genetic diversity found in our donor population reflects the province demographics, particularly in the Montreal area where most of the positive donors were from.

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“…Fifty‐four mutations presented in the a determinant are associated with the atypical profile of simultaneous presence of HBsAg and anti‐HBs markers. Among these mutations, the G145R (glycine‐to‐arginine substitution at position 145 associated with hepatitis B vaccination and the use of HBIG) and I126S (isoleucine‐to‐serine substitution at position 126 related to chronic infection that alters the antigenicity of HBsAg, and immune escape) are the most frequently described until now [Cuestas et al, ; Lada et al, ; Lu et al, ; Mathet et al, ; Colson et al, ; Sayiner et al, ; Zhang et al, ; Velu et al, ; Jang et al, ; Hsu et al, ; Huang et al, ; van Dommelen et al, ; Wang et al, ; Chen et al, ; Liu et al, ; Hsu et al, ; Ding et al, ; Pancher et al, ; Pu et al, ; Ding et al, ; Liu et al, ].…”

Section: Introductionmentioning

confidence: 99%

Mutation in the S gene a determinant of the hepatitis B virus associated with concomitant HBsAg and anti‐HBs in a population in Northeastern Brazil

Albuquerque

Sousa

Santos

et al. 2016

Journal of Medical Virology

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Mutations in the a determinant of S gene may develop co-existence of hepatitis B surface antigen (HBsAg) and antibodies to HBsAg (anti-HBs) in the serum of infected hepatitis B virus (HBV) individuals. Mutations in this region may change the antigenicity of HBsAg, which in turn, lead to escape of neutralizing action of anti-HBs antibodies. This study identified individuals with concomitant HBsAg and anti-HBs serological markers in individuals of Maranhão, Northeastern Brazil. Samples from a population-based study were evaluated for HBsAg, anti-HBs, and anti-HBc, and those that tested positive for simultaneous HBsAg and anti-HBs were submitted to HBV DNA quantification and S gene characterization by Sanger sequencing. Mutations were investigated in the a determinant located in major hydrophilic region (MHR) of the S gene. Among 3,984 samples analyzed, 92 (2.3%) were positive for HBsAg and three had the atypical HBsAg and anti-HBs-positive profile (3.26%). The frequency of HBsAg and anti-HBs co-existence was similar to previous studies. Only one individual harbored mutation in the S gene a determinant associated with this profile. Little is known about this phenomenon; however, studies as ours may contribute for future enlightenment of this important issue. J. Med. Virol. 89:458-462, 2017. © 2016 Wiley Periodicals, Inc.

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A new hepatitis B virus vaccine escape mutation in a renal transplant recipient

Cited by 16 publications

References 18 publications

Naturally occurring MHR variants in Turkish patients infected with hepatitis B virus

Naturally occurring MHR variants in Turkish patients infected with hepatitis B virus

Detection and characterization of hepatitis B virus of anti‐hepatitis B core antigen–reactive blood donors in Quebec with an in‐house nucleic acid testing assay

Mutation in the S gene a determinant of the hepatitis B virus associated with concomitant HBsAg and anti‐HBs in a population in Northeastern Brazil

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