A new high-performance liquid chromatographic method for determination of warfarin enantiomers

Characterization of warfarin unusual peak profiles on oligoproline chiral high performance liquid chromatography columns

“…It has successively enantioseparated on various CSPs [5] such as polysaccharide derivatives, proteins, brush-types, cyclodextrin, cinchona alkaloid, cellulase (CBH I), and ␣-chymotrypsin [6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Several chiral analysis methods were developed and validated for determination of warfarin enantiomers in plasma [20][21][22][23][24]. Binding characteristics of warfarin enantiomer on human serum albumin (HSA) has been extensively studied for pharmacokinetic and pharmacodynamic purpose, because warfarin is a specific probe of binding site I on HAS [25][26][27].…”

Low-density solvent-based dispersive liquid–liquid microextraction followed by high performance liquid chromatography for determination of warfarin in human plasma

“…Plasma concentration can also be helpful in distinguishing noncompliance from genuine anticoagulant resistance. Therefore, various methods for example high-performance liquid chromatography (HPLC) with ultraviolet detector [1,[5][6][7][8][9], liquid chromatography-tandem mass spectrometry (LC-MS/MS) [10,11], micellar electrokinetic chromatography-electrospray ionizationmass spectrometry (MEKC-ESI-MS) [12] and capillary zone electrophoresis (CZE) [13] have been performed for the determination of warfarin in the biological samples. Due to the low warfarin concentrations and complex matrices, biological samples are not directly analyzed using these approaches.…”

Coupling of acetonitrile deproteinization and salting-out extraction with acetonitrile stacking in chiral capillary electrophoresis for the determination of warfarin enantiomers

“…A major complication associated with the prescription of warfarin is that there is a very large variation in optimal dosage between different patients, which can be attributed to differences in liver metabolism of individual patients, drug-drug interactions and variable ingestion of Vitamin K with food [2]. Moreover, it is important to note that warfarin exist in two different enantiomeric forms ( Fig.…”