A New High-Performance Reagent and Procedure for Latent Bloodstain Detection Based on Luminol Chemiluminescence

Blum, Loı̈c J.; EsperanÇA, Philippe; Rocquefelte, StÉPhanie

doi:10.1080/00085030.2006.10757139

Cited by 42 publications

(23 citation statements)

References 9 publications

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“…Recently, a commercially available patented luminolbased formulation called Bluestar ® Forensic (ROC Import Group, Monte Carlo, Monaco) has become available and has been evaluated against traditional luminol preparations [58,59]. From an examination of the material safety data sheets available at the supplier's website (http://www.bluestar-forensic.com), the key differences in the formulation are the use of urea peroxide as an oxidant and sodium hydroxide to provide the basic conditions required for the reaction.…”

Section: Detection Of Latent Bloodstains With Luminolmentioning

confidence: 99%

Chemical Sensing and Detection in Forensic Science

Lewis

2011

Chemosensors

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Section: Detection Of Latent Bloodstains With Luminolmentioning

confidence: 99%

Chemical Sensing and Detection in Forensic Science

Lewis

2011

Chemosensors

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“…Atualmente, os testes realizados pelos laboratórios forenses nas manchas em questionamento permitem a identificação e confirmação v. 6, n. 3, 17-22, 201717-22, ISSN 2237 da existência ou não de determinado fluído corpóreo [1]. Diante de um crime violento o fluído mais encontrado na cena é o sangue [2,3] e os testes presuntivos utilizados para a sua identificação são caracterizados por possuírem elevada sensibilidade e pouca especificidade [4]. A reação química que ocorre nos testes preliminares se dá pela participação do grupo heme da hemoglobina.…”

Section: Introductionunclassified

A interferência da solução de luminol em teste imunocromatográfico para pesquisa de sangue humano

Vaz¹,

Kobachuck²

2017

Rev. Bras. Crimin.

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ResumoAs manchas de sangue muitas vezes são limpas nas cenas de crime, e um dos métodos para identificação de sangue latente é o luminol, o qual possui alta sensibilidade, porém, pouca especificidade, e por esse motivo, deve ser acompanhado de um método de confirmação. Este estudo teve como objetivo analisar a possível interferência do teste presuntivo luminol, preparado de acordo com a formulação de Grodsky sobre o teste confirmatório imunocromatográfico (WAMA®) para pesquisa de sangue humano e verificar a sensibilidade de ambos os testes. Diferentes diluições de uma amostra de sangue humano foram depositadas sobre fragmentos de tecido de algodão branco, deixadas secar por 24 horas em temperatura ambiente e, em seguida, foram submetidas aos dois métodos. Após as amostras terem sido pulverizadas com luminol, a diluição 1:1.000 não mostrou interferência no teste feito imediatamen te após a pulverização, porém após 24 horas a interferência foi observada. Na diluição 1:10.000 houve interferência imediata no teste imunocromatográfico e o resultado persistiu após 24 horas. Já as diluições de 1: 50.000 e 1:100.000 tanto o controle positivo quanto a amostra pulverizada por luminol apresentaram resultados negativos imediatamente e após 24 horas no teste imunocromatográfico. Resultado positivo para o teste de luminol com emissão de quimiluminescência foi obtido até a concentração 1:50.000 e o teste imunocromatográfico mostrou-se positivo até a diluição 1:10.000, indicando que realmente há uma diferença de sensibilidade entre os métodos.Palavras-Chave: Crime; Sangue; Luminol; Imunocromatografia. AbstractBlood stains are often cleaned the crime scene, and one of the methods for latent blood identification is luminol, which has high sensitivity, but low specificity and therefore must be accompanied by a confirmatory method. This study aimed to analyze the possible interference of the presumptive luminol test, prepared according to Grodsky formulation of the immunoassay confirmatory test (WAMA®) for human blood research and check the sensitivity of both tests. Different dilutions of a human blood sample we re deposited on white cotton fabric fragments, allowed to dry for 24 hours at room temperature and then were subjected to two methods. After the samples have been sprayed with luminol, dilution 1: 1000 showed no interference with the test done immediately afte r the spray, but after 24 hours was observed interference. In the 1: 10,000 dilution was no immediate interference in the immunoassay test and the results persisted after 24 hours. Since dilutions of 1: 50,000 and 1: 100,000 both as positive control sample by spraying luminol showed negative results immediately and after 24 hours in the immunoassay. Positive for the luminol test with chemiluminescence emission was obtained by the concentration of 1:50,000 and the immunoassay was positive up to 1:10,000 dilution, indicating that there really is difference in sensitivity between the methods.

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“…To demonstrate bio-sensing on chip, aqueous sensor droplets containing latent bloodstain reagent [9] and an analyte sample droplet containing a synthetic blood analog (Bluestar Forensic) were propelled and subsequently mixed using ethanol fuel droplets. Chemiluminescence emitted from the reaction of the bloodstain with iron catalyst in the blood analog solution was recorded in a dark room using the same digital camera system as described above.…”

Section: Methodsmentioning

confidence: 99%

Biochemical sensing assays based on coalescence-induced self-propulsion digital microfluidics

Nock

Müller

Sellier

et al. 2013

2013 Seventh International Conference on Sensing Technology (ICST)

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Abstract-This work reports on coalescence-induced selfpropulsion as a driving mechanism to actuate microfluidic droplet assays. We demonstrate multi-droplet translation and assay-type sensing on a digital microfluidics platform by use of surface tension gradients alone. These gradients arise during the coalescence of two droplets of liquid having different compositions and therefore surface tensions. We demonstrate a chemiluminescence blood-detection reaction based on the mixing of two carrier droplets containing solutions of synthetic blood and luminol sensor solution. Presence of iron in the blood solution is recorded using digital imaging and analyzed via offline image processing. The results demonstrate the capability of the propulsion mechanism to propel droplets over several millimeters, thus enabling one to design a new family of chip-based biochemical sensor assays.Microfluidics; sensor assay; chemiluminescence; luminol; image processing.

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A New High-Performance Reagent and Procedure for Latent Bloodstain Detection Based on Luminol Chemiluminescence

Cited by 42 publications

References 9 publications

Chemical Sensing and Detection in Forensic Science

Chemical Sensing and Detection in Forensic Science

A interferência da solução de luminol em teste imunocromatográfico para pesquisa de sangue humano

Biochemical sensing assays based on coalescence-induced self-propulsion digital microfluidics

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